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Articles by W. Li
Total Records ( 9 ) for W. Li
  B Li , X Chu , M Gao and W. Li

The aim of this study was to investigate the pro-apoptotic mechanism of C-phycocyanin (C-PC)-mediated photodynamic therapy (PDT) in a murine tumor model and cultured MCF-7 cells. The mice were divided into four groups: control, He–Ne laser radiation, C-PC treatment, and C-PC treatment + He–Ne laser radiation. The effects of C-PC and/or laser on immune organs, immunocyte proliferation, tumor genesis, and apoptosis-related proteins expressions were investigated by immunohistochemistry, in situ hybridization, MTT, electron microscope, western blot, and immunofluorescence assay. The results showed that He–Ne laser treatment alone showed marginal effects. In C-PC-treated mice, the weight of immune organs, proliferation of immunocytes, and expression of pro-apoptotic Fas protein were increased, whereas the tumor weight and the expressions of anti-apoptotic proteins (NF-B and P53) and CD44 mRNA were comparatively decreased. In vitro, C-PC was able to inhibit MCF-7 cell proliferation and cause ultrastructural changes including microvilli loss, formation of membrane blebs, and chromatin condensation. Moreover, C-PC treatment could activate caspase-9 expression, induce cytochrome c release, and downregulate Bcl-2 expression. When combined with He–Ne laser irradiation, the effects of C-PC treatment were further enhanced. Facilitating the apoptosis signals transduction and finally leading to the apoptosis of MCF-7 cells may be the mechanism of the anti-tumor activities of C-PC-mediated PDT.

  X. Wang , B. Zhou and W. Li
  Memcached is a general-purpose distributed memory caching system that was originally developed by Danga Interactive for Live Journal but is now used by many other sites and It is thought to be one of the most effective solutions to speed up dynamic database-driven websites by caching data and objects in RAM to reduce the number of times that an external data source must be read. Memcached system uses a client-server architecture, it provides its standard memcached protocol to support any types of programming languages. The memcached protocol is simple and very efficient, while it doesn’t support big data objects very well. In this study, the memcached systems were firstly illustrated and then a detailed introduction was provided for the memcached protocol and then the issues of the stock protocol were specified. After that, a new streaming protocol was illustrated which was well designed for big data objects and could be easily integrated into original memcached protocols. Finally, some experiments were done to evaluate the new streaming protocol and finally the new protocol was proved to be very efficient for big data objects storage and it explored new application fields for memcached.
  W. Li , G. Wang , X. Wang and S. Li
  The scheduling algorithm for optimistic replication is important, because it has an extreme effect on replication performance. However, most of the scheduling algorithms are designed for a conventional hard disk device with mechanical disk arms. It may be inefficient when the flash memory which possesses both a higher read/write performance and random access rate is equipped. This study proposed a new flash memory-based scheduling algorithm for optimistic replication: FBSA. It parallelized the write requests on the slave node according to the semantic dependency, thus, full drove the flash memory and improved replication performance. The FBSA was fully implemented under a popular open-source DBMS-MySQL and was proved to show dramatic performance improvement compared with the original scheduling algorithm based on the same hardware and software configurations.
  J.Y. Bai , W. Li , S. Li , W.P. Zhang , C.Q. Gu , X.Y. Hu and G.F. Cheng
  To study the cross-application of the Antibodies (Abs) of Homo sapiens and duck origins which provides the fundamentals for potential application of related Abs, we analyzed their differences by molecular biology techniques and preliminary application in peripheral blood. The similarities of deduced aa sequences of CD8α gene reached to about 32% between cherry valley duck and homo sapiens whereas the extracellular regions reached to about 30%. The result showed the obvious differences in protein hydrophilicity, antigenicity and the possibilities of CD8α extracellular region. This study successfully expressed the extracellular region of cherry valley ducks CD8α (ERCVCD8) sequence and prepared for corresponding Abs. The percentage of control group (group 1) CD8+lymphocytes was relatively stable during different phases of the post-infected with DPV when using rabbit anti-the recombinant protein of extracellular region of cherry valley ducks CD8α (rCVERCD8) serum but the percentage of CD8+lymphocytes was unstable when using anti-Homo sapiens CD8α mAb. When rabbit anti-rCVERCD8 serum was used, the percentage of infection group (group 2) CD8+lymphocytes showed regularity however that of CD8+lymphocytes using rabbit anti-Homo sapiens CD8α Abs was irregular. This study indicats that the identification of cherry valley ducks CD8+lymphocytes using anti-Homo sapiens CD8α Abs has its limitations.
  P. Billam , F. W. Pierson , W. Li , T. LeRoith , R. B. Duncan and X. J. Meng
  As a positive-strand RNA virus, hepatitis E virus (HEV) produces an intermediate negative-strand RNA when it replicates. Thus, the detection of negative-strand viral RNA is indicative of HEV replication. The objective of this study was to develop a negative-strand-specific reverse transcription-PCR (RT-PCR) assay for the identification of extrahepatic sites of HEV replication. Briefly, a 494-bp fragment within the orf1 gene of a chicken strain of HEV (designated avian HEV) was amplified and cloned into a pSK plasmid. A synthetic negative-strand viral RNA was generated from the plasmid by in vitro transcription and was used to standardize the assay. A nested set of primers was designed to amplify a 232-bp fragment of the negative-strand viral RNA. The assay was found to detect up to 10 pg and 10–5 pg of negative-strand HEV RNA in first- and second-round PCRs, respectively. The standardized negative-strand-specific RT-PCR assay was subsequently used to test 13 conveniently obtained tissue specimens collected sequentially on different days postinoculation from chickens experimentally infected with avian HEV. In addition to the liver, the negative-strand-specific RT-PCR assay identified replicative viral RNA in gastrointestinal tissues, including the colorectal, cecal, jejunal, ileal, duodenal, and cecal tonsil tissues. The detection of replicative viral RNA in these tissues indicates that after oral ingestion of the virus, HEV replicates in the gastrointestinal tract before it reaches the liver. This is the first report on the identification of extrahepatic sites of HEV replication in animals after experimental infection via the natural route. The assay should be of value for studying HEV replication and pathogenesis.
  Y. X. Liu , W. Li , H. P. Chen , Y. M. Wei , G. Y. Chen , Y .L. Lu and Y .L. Zheng
  Genetic variation of high-molecular-weight glutenin subunits (HMW-GS), low-molecular-weight glutenin subunit (LMW-GS) genes, waxy locus (Wx) and quality characters, including protein content, wet gluten content, sodium dodecyl sulfate (SDS) sedimentation value and dough rheologic properties, were investigated in sixty-seven Sichuan landraces wheat in China. The relationship between these genetic variation and quality characters were also estimated. Alleles Glu-A1c (98.5%), Glu-B1b (98.5%) and Glu-D1a (100%), were dominant alleles at Glu-A1, Glu-B1 and Glu-D1 locus, respectively. Five, three and five types of different LMW-GS allele compositions were also identified and a (frequencies 74.5%), f (61.2%) and i (55.2%) were the dominant types at the Glu-A3, Glu-B3 and Glu-D3 loci, respectively. Significant difference (p<0.05) between types a and d at Glu-A3 locus were found in protein content, sedimentation value, wet gluten content and stability time, difference (p<0.01) between types g and h at Glu-B3 locus in sedimentation value, development time and breakdown time and difference (p<0.01) between types k and m on wet gluten content. All of the landraces carried the wild type Wx-A1a and Wx-B1a alleles. These information could be useful to marker-assisted select for different end-use quality wheat.
  S. D. Sharples , M. Clark , R. J. Smith , R. J. Ellwood , W. Li and M. G. Somekh
  As the field of laser ultrasonics has matured over the past decade, our main focus has been on developing techniques for characterising materials in ever-finer detail, in terms of both spatial resolution and information content. We have worked to improve both the instrumentation and our understanding of the interactions between light, sound and material properties. This effort results in a range of techniques at different stages of maturity, tied by the theme of laser ultrasonic microscopy; that is imaging the interaction between optically excited and detected ultrasonic waves, and the material under inspection. This paper is a review of the work done so far.
  C. Gao , J. Huan , J. Tang and W. Li
  Although previous studies have demonstrated that stromal-derived factor-1 (SDF-1) played a key role in chronic graft dysfunction (CGD), the precise mechanisms underlying this process are not clear. In this study, SDF-1 was injected into keratinocyte stem cells (KSCs) which were isolated and purified from neonatal C57BL/6 (H-2b) mice. Adenylyl cyclase (AC) activity of KSCs was measured and expressions of the human major histocompatibility complex (MHC) class I chain-related antigens A and B (MICA, MICB) detected by immunofluorescence. Cultured KSCs were negative for IA/IE MHC class II molecules by immunofluorescence, indicating the absence of any contamination with Langerhans cells and certifying the purity of KSCs. Over a 7-day culture period, SDF-1 up-regulated AC activity to 2.783 ± 0.799, which was higher than that of the control group (1.290 ± 0.476; P < .01). Immunostaining showed that KSCs expressed increased amounts of MICA protein (0.790 ± 0.134 versus 0.200 ± 0.022; P < .01) and MICB protein (0.610 ± 0.832 versus 0.230 ± 0.016; P < .01). Mixed lymphocyte reaction assays showed that KSCs cultured with SDF-1 injection for 7 days stimulated allogeneic T-cell proliferation. The data indicated that SDF-1 may accelerate the ultimate rejection of allogeneic keratinocytes by enhancing MIC through the AC signal pathway.
  W. Li , T. Xu , J.F. Wang , X.F. Wu , M. Li and P.Y. Lu
  Hematopoietic stem cell (HSC) gene transfer has been attempted almost entirely ex vivo and has been limited by loss of self-renewal capacity and transplantation-related defects in homing and engraftment. Herein we have attempted to overcome these limitations by injecting vectors directly into the bone marrow (BM) to transduce HSCs in their native environment. Simian virus 40 (SV40)-derived gene delivery vectors were used because they efficiently transduce resting CD34+ cells. Neonatal C57BL/6 (H-2b) mice (3 days old) received SV(Nef-FLAG), carrying FLAG marker epitope directly into both femoral marrow cavities. Keratinocyte stem cells (KSCs) were purified at 7 and 14 days after SV40 injection. The KSCs from 10-day-old C57BL/6 mice were designated as controls. Flow cytometric (FCM) analyses indicated that KSCs from transgenic mice showed strong down-regulation of surface immunological molecules CD40, CD80, CD86, and human major histocompatibility complex class I chain-related antigen A (MICA). Mixed lymphocyte reaction (MLR) assays showed that transgenic KSCs depressed allogeneic T-cell proliferation. Immunofluorescence showed transgenic KSCs expressed FLAG for the entire study as well as high levels of transforming growth factor (TGF)-β and BCL-2. Thus, direct intramarrow administration of recombinant SV40 yielded efficient gene transfer to mice BM progenitor cells. KSCs with low immunogenicity may be obtained for further investigations of skin transplantation immunity.
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