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Articles by W. Han
Total Records ( 4 ) for W. Han
  M. Song , W. Han , H. Bao , C. Liu , C. Wu and C. Zhao
  In the present study, embryo liver tissues collected from hypoxic incubation of Tibet chicken and Shouguang chicken were analyzed on their histological structures and antioxidant capacity as well as differential proteomics to study the mechanism of Tibet chicken’s adaptability to hypoxia. The results of histological study conducted with paraffin and ultrathin sections showed that Shouguang chicken had a weaker liver structure with less cell layers and the development of its liver was more impaired compared with Tibet chicken and Tibet chicken maintained relatively better mitochondria than Shouguang chicken. The content of Maleic dialdehyde in Tibet chicken was lower than Shougguang chicken, but the total antioxidant capacity and the content of Superoxide Dismutase in Tibet chicken were higher than Shouguang chicken. Using two-dimensional electrophoresis and mass spectrometry, we identified 8 differential expression liver proteins between Tibet chicken and Shouguang chicken, in which 6 proteins (enolase I, pyruvate dehydrogenase, ATP synthase, ubiquinol-cytochrome C reductase, superoxide dismutase and apolipoprotein A-I) showed higher expression level in Tibet chicken than that in Shouguang chicken while the expressions of the other 2 proteins (Triose phosphate isomerase, adenosine 5-diphosphosugar pyrophosphatase) were less in livers of Tibet chicken. These results indicated that Tibet chicken had higher glucose oxidation level, electron transfer efficiency and antioxidant ability than Shouguang chicken under the hypoxic condition and these differences made Tibet chicken have better adaptability to hypoxia than Lowland chicken. The study made a good basis for the further study of the genetic mechanism of adaptation to hypoxia.
  Y Wang , J Li , Y Cui , T Li , K. M Ng , H Geng , H Li , X. s Shu , W Liu , B Luo , Q Zhang , T. S. K Mok , W Zheng , X Qiu , G Srivastava , J Yu , J. J.Y Sung , A. T.C Chan , D Ma , Q Tao and W. Han

Closely located at the tumor suppressor locus 16q22.1, CKLF-like MARVEL transmembrane domain-containing member 3 and 4 (CMTM3 and CMTM4) encode two CMTM family proteins, which link chemokines and the transmembrane-4 superfamily. In contrast to the broad expression of both CMTM3 and CMTM4 in normal human adult tissues, only CMTM3 is silenced or down-regulated in common carcinoma (gastric, breast, nasopharyngeal, esophageal, and colon) cell lines and primary tumors. CMTM3 methylation was not detected in normal epithelial cell lines and tissues, with weak methylation present in only 5 of 35 (14%) gastric cancer adjacent normal tissues. Furthermore, immunohistochemistry showed that CMTM3 protein was absent in 12 of 35 (34%) gastric and 1 of 2 colorectal tumors, which was well correlated with its methylation status. The silencing of CMTM3 is due to aberrant promoter CpG methylation that could be reversed by pharmacologic demethylation. Ectopic expression of CMTM3 strongly suppressed the colony formation of carcinoma cell lines. In addition, CMTM3 inhibited tumor cell growth and induced apoptosis with caspase-3 activation. Thus, CMTM3 exerts tumor-suppressive functions in tumor cells, with frequent epigenetic inactivation by promoter CpG methylation in common carcinomas. [Cancer Res 2009;69(12):5194–201]

  X.S. Wu , W.B. Bao , J.T. Shu , Q. Xu , X.Y. Zhang , W. Han and G.H. Chen
  This study was designed to investigate the effect of Adenylosuccinate Lyase (ADSL) gene on Inosine Monophosphate content (IMP) in chicken. The chickens used for the study included exogenous Recessive White chicken and Chinese indigenous chicken breeds such as Silkies, Baier, Tibetan and Xiaoshan chickens. A pair of primer was designed to detect exon 2 of ADSL gene by PCR-SSCP and mutation detected was directly sequenced. A synonymous mutation at 3484 bp in exon 2 was substituted C/T SNP. In five chicken breeds, three genotypes TT, CT and CC were observed in exon 2. Muscle IMP content of individuals with TT genotype in exon 2 had significantly higher than those with CT and CC genotypes (p<0.05). In exon 2, Recessive White, Xiaoshan and Baier chickens were in Hardy-Weinberg equilibrium (p>0.05) and Silkies and Tibetan chickens were significant departure from Hardy-Weinberg equilibrium (p<0.01).
  Y.F. Zhu , H.F. Li , W. Han , J.T. Shu , W.T. Song , X.Y. Zhang and K.W. Chen
  The objective of this study was to trace the route of avian Mx (myxo-virus resistance) gene evolution and knowledge of its structure and function variations. Mx gene cDNA sequences of six galliform and anseriform avians were used in this study. Datamonkey and DAMBE were used to detect recombination events and test nucleotide substitution saturation. PAML4b were used to test the selective pressure on amino acid sites. Saturation test did not indicate any sign of substitution saturation. Single breakpoint scanning and genetic algorithm scanning by Datamonkey found two breakpoints, located, respectively in 162 and 999 bp and divided the sequences into three nonrecombination split partitions. The selection test of site-specific mode showed that avian Mx gene sequences had suffered positive selection pressure. Likelihood Ratio Test (LTR) suggested M2a and M8 be more advantageous models,and in a total of eight positive sites with >95% posterior probability were identified in three nonrecombination split partitions. These detected positive sites distributed in N-ternimal and GTP-binding domain of Mx protein and might be important candidate marks for improving avian antiviral activity.
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