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Articles by W. Anwer
Total Records ( 3 ) for W. Anwer
  Z. Moustafa Gehan , W. Anwer , H.M. Amer , I.M. EL-Sabagh , A. Rezk and E.M. Badawy
  Studies have indicated variations in the degree of efficacy of the commercial disinfectants commonly used in poultry production facilities. An adequate method of in vitro testing was used to compare the efficacy of some of these disinfectants while testing them in conditions similar to those of the poultry facilities. Five commercially available disinfectants were tested against 7 selected bacterial, fungal and viral isolates. The obtained results indicated that, most of the tested disinfectant products were effective at the manufacturer recommended level within 30 min contact time when tested in the absence of organic matter. However, when organic matter was present longer contact times were needed to demonstrate the effectiveness. Pseudomonas aeruginosa, Fusarium species and Newcastle disease virus showed variable degrees of resistance to some of the tested disinfectant products in the presence of organic matter. Conclusively, monitoring program should be adopted regularly in poultry facilities to test the problematic microbes individually for their resistance against commercial disinfectants.
  H.M. Agouz and W. Anwer
  A commercial probiotic (Biogen®) was tested against a commercial mycotoxin binder (Myco-Ad®) for their effects on survival, growth, body composition and hematological picture of common carp (cyprinus carpio) fed a naturally contaminated aquafeed with aflatoxin and ochratoxin. A total number of 150 apparently healthy fingerlings common carp cyprinus carpio were divided into 5 triplicate groups. G-1 (control) received naturally ration found contaminated with aflatoxin (22 ppb) and ochratoxin (15 ppb). The other four groups supplemented with Biogen® at a rate of 0.2 and 0.4% (G-2 and G-3) and Myco-Ad® at a rate of 0.15 and 0.25% (G-4 and G-5). Results showed a significant reduction (p≤0.05) in aflatoxin recorded in aquafeeds of G-2 and G-3, while ochratoxin level showed a significant reduction in G-3. The four groups received Biogen® and Myco-Ad® showed a significant improve in the final weight, feed intake, FCR, survival rate, protein efficiency, body composition represented an increase in crude protein and reduction of ether extract and also improve hematological picture represented in erythrocyte counts, hematocrite and hemoglobin. It can be concluded that aflatoxin and ochratoxin contamination of fish diets can cause many drastic effects on performance parameters, feed utilization, hematological picture and body composition of common carp. Hence, there is always a demand for risk assessment regarding mycotoxins especially with the moving to plant protein sources in the aquafeeds. Commercial probiotic Biogen® at a level of 0.4 and 0.2% has a good improvement effect among common carp fed mycotoxin contaminated aquafeed, followed by the commercial mycotoxin binder Myco-Ad® at a level of 0.25 and 0.15%.
  A. Aly Salwa and W. Anwer
  The aim of this study was to evaluate the effect of naturally contaminated feed with aflatoxin on performance of laying hens fed for 60 days and the carryover of AFB1 residues in eggs as well as the stability of AFB1 in naturally contaminated eggs to boiling process. Forty, 30 weeks old, White Leghorn laying hens were randomly assigned into four experimental groups and after 2 weeks were given naturally contaminated feed containing zero (control), 25, 50 and 100 µg aflatoxin/kg feed. Twenty eggs per treatment were collected on days (1-7); 10; 20, 30, 40, 50 and 60 and submitted to aflatoxin B1 analysis using ELISA. Average egg production and egg weight were not affected by aflatoxin (P>0.05), while a significant decrease in feed intake (p<0.05) was appeared in the 2 groups fed on 50 and 100 aflatoxin ug/kg feed. Residues of aflatoxin B1 were detected in eggs at levels that ranged from 0.02 to 0.09 with a mean value of 0.04, 0.05 and 0.07 µg/kg respectively. Aflatoxin B1 was almost stable in naturally contaminated egg for up to 20 minutes of boiling, so avoiding aflatoxin B1 transmission into egg appears to be the only practical way to ensure their safety for human consumption. Conclusively, the excretion of aflatoxin B1 residues in hens` eggs might occur at relatively low concentrations under conditions of long term exposure of laying hens to low level of aflatoxin in naturally contaminated feed with reduction in feed intake started at 50 µg/kg.
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