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Articles by Vinod Chhokar
Total Records ( 3 ) for Vinod Chhokar
  Anil Kumar , Vinod Chhokar , Ravinder Kumar , Himanshu Aggarwal and Vikas Beniwal
  Background: Triacylglycerols are quantitatively most important storage form of energy for eukaryotic cells. Acyl CoA: Diacylglycerol O-acyltransferase 1 (DGAT1, EC 2.3.1.20) is a microsomal enzyme that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Objective: The present study was undertaken with the objectives to study the molecular differences in DGAT1 among different ruminants. Materials and Methods: The DGAT1 was partially amplified (411 bp) by designing gene specific primers and confirmed by sequencing the amplicon and its comparison with DGAT1 gene of other ruminants (i.e., bovine, buffalo and goat). Results: Comparative study of DGAT1 among different ruminants reveals different level of mutation with respect to its gene sequence (0.0-0.4%) and protein sequence (0.0-1.2%). Conclusion: Molecular differentiation among different ruminants reveals that bovine and buffalo are very close to each other and sheep is related to goat. Further study is underway to detect polymorphism and associated them with milk fat trait in sheep.
  Himanshu Aggarwal , Gyanprakash , Alka Rao and Vinod Chhokar
  The root extract of Asparagus racemosus was studied in vitro against five strains of bacteria by using agar well/disc-diffusion method and growth inhibition in liquid culture. Among the three different extraction methods namely, cold maceration method, soxhlet extraction and reflux extraction that were employed for the extraction of bioactive components from the roots of the A. racemosus, the methanolic extract prepared by reflux extraction was found to have the maximum antibacterial activity against Escherichia coli DH5α and Pseudomonas aeruginosa (MTCC1688). The MIC value of methanolic A. racemosus Root Extract (ARE) as determined by agar disc diffusion assay was found considerably significant for a crude extract at 0.5 mg mL-1 against P. aeruginosa (MTCC1688) and as low as 0.25 mg mL-1 in case of E. coli DH5α in comparison with the synthetic antibiotic ampicillin.
  Himanshu Aggarwal , Alka Rao , J.S. Rana , Jasbir Singh , Anil Kumar , Vinod Chhokar and Vikas Beniwal
  The present investigation was carried out to access the suitability and reliability of ISSR analysis for inferring genetic diversity among chickpea genotypes. A total of six ISSR markers were used to study genetic diversity among 115 genotypes of chickpea. Out of six ISSR markers (GTGC)4 (GTG)5 and (TCC)5 were found to generate reproducible DNA fragments of size ranging from 0.15-3, 0.40-2.5 and 0.35-2 kb and yielded 2-9, 3-10 and 4-11 number of fragments, respectively. Clustering analysis separated all the genotypes into five genetically similar groups. The value of cophenetic correlation coefficient (r) is 0.91 which is considered as a good fit. The genetic similarity coefficient among 115 chickpea genotypes ranged from 0.01 to 0.90. These values indicated high genetic variability among chickpea cultivars and can be used efficiently for gene tagging and genome mapping of crosses to introgress the favourable traits such as high yield potential, disease and insect resistance into the cultivated genotypes.
 
 
 
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