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| Articles
by
Vinay Goyal |
Total Records (
1 ) for
Vinay Goyal |
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Suhail Rasool
,
Madhuri Behari
,
M. Irshad
,
Vinay Goyal
and
B.L. Jailkhani
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Using α-bungarotoxin (α -Bgtx) and β -bungarotoxin
(β -Bgtx) to capture their corresponding proteins from preparation
of crude human muscle receptor (triton extract), antibodies against these
corresponding proteins were detected in Myasthenia gravis patients. α-Bgtx
binds to AChR and β -Bgtx binds to presynaptic membrane receptor (PsmR)
of triton extract. The captured proteins were used as antigen in indirect
ELISA to detect the IgG antibodies against AChR and PsmR in sera from
146 Indian patients with MG and in 30 controls. Out of 146, 130 were generalised
myasthenic patients (GM), 16 ocular Myasthenic patients (OMG). In addition
to AChR antibodies, Myasthenia Gravis (MG) patients were also showing
antibodies against PsmR. The frequency of anti-AChR antibodies was 71%
and anti-PsmR antibodies was 65%. The OD values of GMG for AChR and PsmR
were (0.76 ± 0.34) and (0.75 ±0.37), for OMG patients (0.32 ± 0.1)
and (0.72 ± 0.42) and for healthy controls (0.16 ±0.06) and
(0.18 ±0.04), respectively. These results showed that MG is not
only due to the damage of the postsynaptic membrane, but it could concurrently
be a consequence of antibody mediated damage of the presynaptic membrane,
resulting particularly the damage of its β-Bgtx binding sites and
could play an important role in the pathogenesis of MG. Therefore, it
is also necessary to detect antibodies against PsmR in addition to AChR
antibody. |
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