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Articles by Vildan AVKAN OGUZ
Total Records ( 2 ) for Vildan AVKAN OGUZ
  Vildan AVKAN OGUZ , Nurbanu SEZAK , Aygun OZTOP , Nur YAPAR , Suheyla SURUCUOGLU and Ayse YUCE
  Aim: The early diagnosis of active tuberculosis still depends on the presence of acid-fast bacilli (AFB) in stained sputum smears. In this study, our aim was to investigate the efficiency and cost-effectiveness of two different fluorochrome stains.
Materials and methods:
A total of 1013 sputum specimens were collected from 642 patients. Three smears and cultures were prepared from each specimen. Double-blind and prospective laboratory procedures were performed. Slides were stained with a commercial auramine/acridine orange kit (Stain 1), an in-house preparation of auramine- rhodamine/KMnO4 (Stain 2) and a Ziehl-Neelsen stain (EZN).
Results:
Of the 1013 specimens, 101 were culture positive. Among these, AFB was detected in 60 specimens by EZN, in 53 by Stain 1, in 81 by Stain 2. By cultures, the sensitivities and specificities of Stain 2 were 80.1% and 83.8%, respectively, and for Stain 1, 52.4% and 94.6% respectively. There is no significant difference between the costs of these methods.
Conclusion:
Stain 1 was easy to apply and inexpensive but the sensitivity of Stain 1 was lower than that of Stain 2. However, Stain 2 required longer preparation time, more work, and had a higher risk of exposure to carcinogens. In order to increase the sensitivity of Stain 1, it is suggested that the contents of the prepared Stain 1 kit could be rearranged. In tuberculosis diagnosis, this revised kit may provide practicality in use.
  Nur YAPAR and Vildan AVKAN OGUZ
  Aim: Nasal carriage rates for Staphylococcus aureus have been reported to vary between 18% and 50% in different populations and it represents a risk factor for invasive infections. The aim of the present study was to investigate the presence of genes associated with fibronectin binding proteins mediating the adhesion of S. aureus to human epithelial cells.
Materials and methods:
Fifty strains isolated from nasal swab specimens of children were included. Specimens were inoculated on mannitol salt agar plates and after 24-48 h of incubation at 37 °C the isolates were identified according to their biochemical properties. The presence of fnbA and fnbB genes encoding fibronectin binding protein A and B was investigated via PCR. S. aureus NCTC8325 was used as the reference strain.
Results:
All isolates were identified as S. aureus according to their cultural properties and the positive tube coagulase test results. Of the 50 S. aureus strains, 14 (28%) were positive for fnbA and 5 (10%) for fnbB.
Conclusion:
The presence of fnbA and fnbB in our study population was lower than in previous studies performed in nasal carriers. We concluded that this difference might have resulted from the lower age of the study population and geographical diversities.
 
 
 
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