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Articles by Vikrant Sudan
Total Records ( 2 ) for Vikrant Sudan
  Amit Kumar Jaiswal , Vikrant Sudan , Neha and Amit Kumar Verma
  Trypanosomiasis is a haemoprotozoan disease entity caused by various members of Trypanosoma spp. affecting different species of domestic and wild animals like horses, mule, donkey, camel, cattle, buffaloes, sheep, goat, dogs, pig, elephant, deer, foxes, tiger and jackals with chief clinical signs of high intermittent fever, anaemia, loss of weight, edema of dependent parts, nervous symptoms, abortion and is responsible for major production losses. It can be transmitted through the biting flies wherein, the parasite may undergo biological or mechanical transmission. Various diagnostic methods from traditional to molecular are available for its diagnosis viz., microscopic examination, DNA detection by PCR, Card Agglutination Test and ELISA. The present review addresses important insights into trypanosomiasis, the etiological agent, advances and trends in its diagnosis, treatment aspects, prevention and control measures; which would help in limiting its prevalence in animals.
  Sanjhi Paliwal , Vikrant Sudan , Daya Shanker and Mukesh Srivastava
  Background and Objective: Knowledge of local isolates and strains is a prerequisite for development of both effective mass vaccination strategy and a suitable molecular marker based diagnostic tool. The pathogenesis of bovine tropical theileriosis (BTT) caused by Theileria annulata in susceptible ruminants is known to vary depending upon the isolate and strain involved. The present study was designed to characterize T. annulata isolates from Northern India using PCR-RFLP technique. Materials and Methods: In the present study, HSP 70 gene was amplified from 28 naturally infected calves found in BTT endemic semi arid zone of Northern India. The amplified product was then digested with two restrictions enzymes viz., Taq I and Alu I to find out the pattern of restriction digests so as to have an idea of various strains of T. annulata involved. Results: With Taq I enzyme, two restriction digestion products were noticed with molecular weights of 100 and 175 bp, respectively. So far as Alu I enzyme is concerned, three restriction digestion products with molecular weights of 60, 90 and 125 bp, respectively, were noticed. Conclusion: Monomorphic pattern of restriction digest was noticed in all 28 studied T. annulata isolates, for both the enzymes, suggesting of presence of single strain of parasite involved in the studied area.
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