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Articles by Vikineswary Sabaratnam
Total Records ( 4 ) for Vikineswary Sabaratnam
  Norazli Ghadin , Noraziah M. Zin , Vikineswary Sabaratnam , Norhidayah Badya , Dayang F. Basri , Hing H. Lian and Nik M. Sidik
  In search of new antimicrobial agents, samples of plant stem which were originally used as traditional medicine were collected from various parts of peninsular Malaysia. The isolate, namely SUK 06 from Thottea grandiflora (family-Aristolchieaceae) was cultured on International Streptomyces Project medium (ISP 2). Macroscopically, the bacteria surface appeared to be smooth with granule texture, irregular folding, dry and chalky and showed a substrate mycelium with grey in color. By scanning electron microscope, this isolate had a unique spiral filament structure. Molecular biological studies on the 16S rRNA revealed that it was distinct from all other genetic accessions of Streptomycetes in GenBank, but bear some genetic similarity to other Streptomycetes. The secondary metabolites extracted by ethyl acetate showed demonstrable killing activity against one or more pathogenic bacteria and inhibition zones (mm) were as followed: Bacilus subtilis (17 mm), Pseudomonas aeruginosa ATCC 27853 (20 mm), Bacillus cereus (22 mm), Pleisiomonas shigelloides (20 mm) and MRSA ATCC 700699 (37 mm). Nevertheless, there were some antifungal activity measured as a percentage of inhibition (%) against Fusarium solani (62%), Aspergillus fumigatus (44%), Pythium ultimum (20%), Phytophthora erythroseptica (23%) and Geothrichum candidum (23%).
  Umaiyal Munusamy , Vikineswary Sabaratnam , Sekaran Muniandy , Noorlidah Abdullah , Ashok Pandey and E.B.G. Jones
  Four strains of Pycnoporus sanguineus isolated from Thailand (KUM 60953), Shah Alam (KUM 60954), Endau Rompin (KUM 60955), Cherating (KUM 60956) and Gombak (KUM 60957) were grown on PDA plates. Laccase of P. sanguineus was produced using oil palm frond parenchyma tissue (OPFPt) in solid substrate fermentation (SSF). Strain KUM 60954 produced significant (p = 0) levels of laccase at 2.53 U mL-1 (76 U g-1) followed by KUM 60957 at 1.05 U mL-1 (32 U g-1), KUM 60956 at 0.55 U mL-1 (17 U g-1) and KUM 60955 at 0.46 U mL-1 (14 U g-1). Meanwhile, KUM 60953 (reference strain) from Thailand produced 2.44 U mL-1 of laccase (73 U g-1 of OPFPt). Biodegradation of a mixture of 10 ppm each of phenanthrene, anthracene and pyrene by 30 U mL-1 of laccase in sodium citrate buffer pH 5 was studied. The reaction mixture was incubated at 40°C and was shaken at 80 rpm for 24 h. Laccase of KUM 60954 degrades 90% of phenanthrene, 37% of anthracene and 96% of pyrene. Meanwhile, laccase of KUM 60953 degrades 89% of phenanthrene, 43% of anthracene and 95% pyrene. Pyrene was rapidly biodegraded followed by phenanthrene and anthracene. However, a similar pattern of degradation was observed for both KUM 60953 and KUM 60954. Degraded PAH sample was further tested for toxicity using Artemia. The untreated PAH caused more than 50% of Artemia death while for the treated PAH no death was observed indicating that the toxicity level was reduced and possibly no any new toxic compound was produced during the degradation.
  Umaiyal Munusamy , Vikineswary Sabaratnam , Sekaran Muniandy , Noorlidah Abdullah , Ashok Pandey and E.B.G. Jones
  Four strains of Pycnoporus sanguineus isolated from Shah Alam (KUM 60954), Endau Rompin (KUM 60955), Cherating (KUM 60956), Gombak (KUM 60957) and the standard strain from Thailand (KUM 60953) were evaluated for their ability to grow on Polycyclic Aromatic Hydrocarbon (PAH)- and acetonitrile-incorporated Potato Dextrose Agar (PDA). The mycelial growth was then compared to the growth of P. sanguineus on PAH and acetonitrile free PDA. All strains tested showed a high tolerance to PAH up to 25 ppm and 2.5% of acetonitrile. Laccase derived from the best strains KUM 60954 and KUM 60953 which exhibited higher tolerance to both PAH and acetonitrile was further characterised. The activity was maximum for both strains at pH 5.0 and 40 °C. Laccase from both strains was stable over a wide range of pH (3-5) and at temperatures below 60 °C. Laccase was stored as a concentrate or as a lyophilised powder to minimise the degradation of enzyme activity during storage. The laccase activity in the extract remained a high activity up to four months at optimum pH 5.0 and at both 0 and 4 °C. Further laccase was also stable up to 24 h at 40 °C when mixed with 1% of acetonitrile.
  Wong Kok Mun , Nor`Aini Abdul Rahman , Suraini Abd-Aziz , Vikineswary Sabaratnam and Mohd Ali Hassan
  In order to optimize the enzymatic hydrolysis of POME solid, the effects of substrate pretreatment using varying concentrations of sodium hydroxide and sulfuric acid, crude enzyme from both strains in different ratio and pH reaction were studied. The best experimental conditions found to degrade POME solids were 12 h incubation time, 0.5% (v/v) sulfuric acid pretreatment, crude enzymes mixture from Aspergillus niger EB5 and Trichoderma sp. EB6 (1.75 mL Asp+0.25 mL Tri with the total cellulase activity equal to 14.76 IU) and incubation pH at 5.0. Under these conditions, the reducing sugar concentration reached 23 g L-1 with the hydrolysis yield and productivity at 32% and 1.90 g L-1 h-1, respectively. The bioconversion of POME solid to reducing sugar by the mixture of crude enzyme from the strains was relatively higher by almost 2 folds as compared to commercial cellulase. The results suggested that the crude cellulases mixture from locally isolated fungi has potential for hydrolyzing the abundant agriculture residues from the palm oil industry.
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