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Articles by Veriani Aprilia
Total Records ( 2 ) for Veriani Aprilia
  Veriani Aprilia , Agnes Murdiati , Pudji Hastuti and Eni Harmayani
  Objective: This study was conducted to describe the synthesis of carboxymethyl porang glucomannan (CPGM) and its physicochemical characteristics. Materials and Methods: The CPGM was synthesized by treating porang glucomannan (PGM) with sodium chloroacetate under basic conditions at different temperatures (50, 60 and 70°C) for different durations (20, 40 and 60 min). The CPGM products were then analyzed to determine what functional groups were present, the degree of substitution (DS), the water solubility, the zeta potential and the viscosity. Results: Carboxymethylation of the porang glucomannan was confirmed by the increase in the intensity of the carbonyl absorption peak in the fourier transform infrared (FTIR) spectra. Compared to the native porang glucomannan, the carboxymethylated porang glucomannan was more negatively charged and more water soluble but was less viscous. Both temperature and reaction time influenced the viscosity and water solubility, but the DS value was more influenced by reaction time than by temperature. Conclusion: Carboxymethylation of porang glucomannan yielded a more useful product than did native porang glucomannan. The increase in water solubility and decrease in viscosity made the product widely applicable in the food industry. The negatively charged polymer has the potential to interact with positively charged polymers to develop new products. Further research may be conducted to tune the reaction time to achieve certain properties that will suit additional needs.
  Veriani Aprilia , Agnes Murdiati , Pudji Hastuti and Eni Harmayani
  Background and Objective: Probiotic is functional food gave good effect to human body if it is consumed in adequate amount. Its viability becomes lower during processing, storing and delivering to the body. Therefore, it needs to be protected. Hydrogel formed from interaction between glucomannan and chitosan may protect it. The aims of this study were to evaluate the characteristics of hydrogel made from glucomannan and chitosan and its efficiency in encapsulating Lactobacillus acidophilus FNCC 0051. Materials and Methods: Hydrogel was prepared by extruding 0.5% w/v chitosan in 1% acetic acid to 0.5% w/v carboxymethyl glucomannan. Properties of hydrogel such as particle size, morphology Fourier Transform Infra Red (FTIR) spectra and swelling ratio were measured. Encapsulation efficiencies were evaluated by enumerating encapsulated and unencapsulated cells. Data were analyzed using one way-ANOVA, then continued with Duncan’s Multiple Range Test (DMRT). Results: The result of the study showed that hydrogel formed using complex coacervation between glucomannan and chitosan had the spherical shape with the particle sizes around 1.09-2.31 μm. The new peak spectra at 1589 cm–1 confirmed that L. acidophilus was encapsulated in hydrogel matrix. The hydrogel respectively showed minimum and maximum swelling ratio at pH values of 5 and 8. Encapsulation efficiency was 66.1±3.0% when the cells were released in pH 8. It was not significantly different to the efficiency in pH 7. Enumerating unencapsulated cells provided lower encapsulation efficiency. Conclusions: L. acidophilus can be encapsulated in hydrogel using complex coacervation of glucomannan and chitosan. The low swelling ratio at lower pH provide better protection for the cell in the stomach, while the high swelling ratio at higher pH may be used to release the cell in small intestine. Further researches may be conducted to increase the encapsulation efficiency.
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