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Articles by Vera Maria Barbosa de Moraes
Total Records ( 2 ) for Vera Maria Barbosa de Moraes
  Fabiana Silva Lima , Elizabeth Santin , Antonio Carlos Paulillo , Luciano Doretto Junior , Vera Maria Barbosa de Moraes , Nilce Maria Queiroz Gama and Ruben Pablo Schocken - Iturrino
  The objective of this study was to evaluate different programs of the vaccination against Newcastle disease in Japanese quails. Two hundred and eighty eight 5-week-old Japanese quails were distributed into six different vaccine programs: T1 - vaccinated with LaSota strain of Newcastle virus (NDV) via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks with NDV LaSota strain via intra-conjunctiva instillation; T2 - vaccinated with NDV B1 strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks of NDV B1 strain via intra-conjunctiva instillation; T3 - vaccinated with NDV Ulster 2C strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks of age with Ulster 2C strain; T4 - vaccinated with NDV VG-GA strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks with NDV VG-GA strain via intra-conjunctiva instillation; T5 - vaccinated with NDV LaSota strain, which was inactivated and emulsified in mineral oil, subcutaneous, at five weeks of age and were not revaccinated; and T6 - not vaccinated. At 17 and 25 weeks of age, all birds were challenged with a velogenic strain of NDV and a group of specific pathogen free (SPF) broilers was used as control of the virus. Five and 14 days after challenge, tracheal and cloacal swabs were collected from each bird for virus isolation. The quails from all experimental groups did not show any clinical sign of NDV, although 100% of SPF broilers that died after the challenge showed clinical signs of Newcastle disease. NDV isolation was possible in all SPF broilers and 5 and 14 days after challenge in the non-vaccinated group of quails (T6), suggesting that quails can be NDV carriers. In the vaccinated groups, NDV was not re-isolated, demonstrating the importance of vaccination to control virus dissemination by quails infected with NDV.
  Fabiana Silva Lima , Elizabeth Santin , Antonio Carlos Paulillo , Luciano Doretto Junior , Vera Maria Barbosa de Moraes and Ruben Pablo Schocken - Iturrino
  This study was carried out to clarify the real role that was played by the quail (Coturnix coturnix japonica) in the epidemiological plan, under the perspective as infection source of the Newcastle disease virus (NDV). For this, they used specific-pathogen-free birds (SPF) that were housed with inoculated quails with a pathogenic strain (velogenic viscerotropic), of NDV (DIE50=108,15/0.1 ml), by the ocular-nasal via. Each group was composed by 6 SPF birds and 12 Japanese quails. After 4, 14 and 20 days since the inoculation of the quails with NDV, 6 SPF birds were put together with each group of quails, so that there was a direct contact among the species. After 5, 14 and 20 days since the challenge of the quails with NDV, the samples were collected through the vent swabs of the quails for the viral isolation (pathogenic virus) in SPF embryos. There was no clinical disease in the quails inoculated with NDV. Therefore, there was viral isolation from 5 to 14 days after the challenge with the NDV, demonstrating, this way the state of carrier of the quail NDV, which happened until 14 days of the experimental infection with this pathogen. So, 100% of the SPF birds which housed with the NDV infected quail, after 5 (group 1) and 14 days (group 2), died from 4 to 7 days after the direct contact among species. This way, the transmission of the pathogenic virus of the quail was evident until 14 days of the experimental infection with this pathogen (NDV) for the SPF birds that housed together, and that calls the attention to the importance of the quail from the epidemiological point of view as potential source of infection of the NDV to commercial birds that housed with these specie or near this breeding.
 
 
 
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