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Articles by V.K. Saxena
Total Records ( 1 ) for V.K. Saxena
  A.S. Yadav , G.K. Saxena , V.K. Saxena and J.M. Kataria
  Salmonella is an important food-borne pathogen associated with poultry. Prevalence of Salmonella was assessed in fecal swab samples collected from broiler birds of native farms in which 5% samples were found positive by conventional and PCR based methods. Out of 200 fecal swab samples 10 samples were Salmonella. Out of these Salmonella isolates, 4 were serotyped as Salmonella Typhimurium, 5 as untypable and 1 as rough strain. Survival of Salmonella Typhimurium and Salmonella Enteritidis was assessed at 30, 42 and 50°C for 24 h under laboratory conditions and results revealed that both serotypes survived at 50°C up to 24 h. Expression profiling of genes conferring survival and thermotolerance to these serotypes was assessed at high temperature using real-time PCR with transcripted RNA, after exposure at 42 and 50°C for 10 and 20 min in a water bath. Transcriptional profiling analyzed to study the relative expression of thermotolerance genes (rpoE, rpoH, rpoS, htrA, uspA and uspB) showed over expression of rpoE, rpoH and htrA genes at 5°C in both serotypes. Since S. Typhimurium exhibited higher expression of various genes when exposed to 50°C for 20 min, it may have better ability to respond to higher temperatures stress compared to Salmonella Enteritidis. Both serovars showed higher attachment to skin after exposure to temperature, which may lead to cross-contamination and foodborne illness. This study will be helpful for the poultry processors to design new intervention strategies for the effective destruction of such type of Salmonella cells on dressed poultry carcasses.
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