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Articles by V. Masilamani
Total Records ( 2 ) for V. Masilamani
  A. Balasundaram , P. Rathna Kumari , A. Stalin , V. Masilamani and George John
  A study was carried out to determine the influence of a commercial probiotic (‘Aqualact’) and dried leaf powder of Cassia auriculata against Vibrio parahaemolyticus infection in the freshwater prawn, Macrobrachium rosenbergii. Histological studies in the hepatopancreas and gills of prawns, during infectivity trials, showed bacterial invasion and multiplication in their lumens. Oedema and cell necrosis were the major pathological changes observed in gills and hepatopancreas. Degenerative changes in the electrophoretic pattern of muscle proteins were proportional to the concentration of vibrios infected. Infected prawns fed on probiotic supplemented feed could show a regaining trend towards normal protein bands. Marked reduction in mortality could be observed in infected prawns fed on feed supplemented with probiotic as well as with powdered Cassia auriculata leaves.
  A. Balasundaram , P. Rathna Kumari , P. Kolanchinathan , V. Masilamani and George John
  The present study evaluated genetic variation in Aeromonas hydrophila strains using PCR-RAPD and their varied susceptibility to phyto-extract. Four strains of Aeromonas hydrophila isolated from skin infections of common freshwater fish, Cyprinus carpio were characterized by various biochemical methods, physiological tests and PCR- RAPD. Antimicrobial activity of the leaf extracts of three medicinal plants, Ocimum sanctum, Adathoda vasica and Calendula officinalis were tested against the four strains of A. hydrophila by disc diffusion (Kirby-Bauer) method. Antagonistic effects of leaf extracts against A. hydrophila strains were assessed by co-culture method. RAPD analysis showed that all the microbes isolated from skin infection belong to the same species but there was no 100% genetic similarity among them Dendrogram constructed by UPGMA clearly supported the PCR pattern of genetic variability among the strains. This study revealed that Aeromonas hydophila exhibits genetic variability and varied susceptibility towards phyto-extracts. Results indicated that phyto-extracts offers a promising alternative to the use of antibiotics in controlling Aeromonas hydrophila.
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