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Articles by V. Jegathambigai
Total Records ( 3 ) for V. Jegathambigai
  V. Jegathambigai , R.S.W. Wijeratnam and R.L.C. Wijesundera
  Crossandra infundibuliformis var. Danica is a very valuable ornamental flowering potted plant that has been introduced to the international floriculture market. A wilt caused by Fusarium oxysporum is a major problem in the production of C. infundibuliformis plants. Control of F. oxysporum causing wilt disease of C. infundibuliformis var. Danica was investigated using Trichoderma isolates. The isolates effected the growth of Fusarium in laboratory experiments. The results suggest that the effect on fungicidal. The Trichoderma isolates also effectively reduced the wilt incidence in field experiments. Further, the isolation promoted the growth of the plants. The study strongly suggests that Trichoderma isolates, especially T. viride Tv1 can be exploited for the biological control of wilt disease at field level.
  V. Jegathambigai , R.S. Wilson Wijeratnam and R.L.C. Wijesundera
  Meloidogyne incognita is a causal agent of root knot disease in many plants. This nematode causing damage in foliage nurseries of Livistona rotudifolia cultivated countries especially in Sri Lanka. Due to importance of this disease in foliage nurseries and impossibility of using chemical control against this damaging agent in this research, two species of Trichoderma viride strain NRRL 6418, Trichoderma harzianum (Hypocrea lixii TWC1), as an antagonist fungi against M. incognita were isolated and identified from foliage nursery in Sri Lanka. The efficacy of these bio control agent against M. incognita were investigated in vitro and in vivo condition. Thus, this research was carried out to investigate the mechanisms of action by which Trichoderma sp. antagonizes M. incognita in vitro and the efficacy of application of a conidia suspension of Trichoderma for control of M. incognita root knot disease under field conditions. In vitro, the antagonistic behavior of Trichoderma sp. resulted in the overgrowth of M. incognita by Trichoderma sp., while the antifungal metabolites of Trichoderma sp. prevented the hatching of M. incognita eggs and the growth of the second juveniles. Light microscope observations showed the evidence that mycoparasitism contributed to the aggressive nature of the tested isolate of T. viride NRRL 6418 and T. harzianum(Hypocrea lixii TWC1) against M. incognita female body and egg masses. Under field conditions, drenching of a conidia suspension of T. viride NRRL 6418 and T. harzianum (Hypocrea lixii) at 1X10(14) spore mL-1 significantly reduced the disease incidence (DI) in the plant roots and also significantly increased the plant growth of L. rotundifolia.
  V. Jegathambigai , R.S. Wilson Wijeratnam and R.L.C. Wijesundera
  The antagonistic effect of three local isolates of Trichoderma viride and one local isolate of Trichoderma harzianum were tested against the pathogenic fungus Sclerotium rolfsii. The latter organism is responsible for major loss due to collar rot of the ornamental crop Zamioculcas zamiifolia in Sri Lanka. The disease causes massive losses. The antagonistic potential of the local isolates against the phytopathogenic fungi Sclerotium rolfsii was investigated in dual culture, poison food technique, pot trials and field trials on Zamioculcas zamiifolia plants. All Trichoderma isolates tested under in-vitro conditions significantly inhibited the growth of S. rolfsii. Of these isolates, Trichoderma viride isolate Tv1, showed highest percentage inhibition and was thus selected for in vivo field trials. Data recorded from bi monthly field application of this organism over the two growing seasons, confirmed the success of the treatment in controlling collar rot disease at the economic threshold level. Field application of testing isolate T. viride Tv1 as a conidial suspension (1011 cfu mL-1) greatly reduced the disease incidence of Zamioculcas zamiifolia plants by a percentage of 75.54%. On farm mass production of this isolate was developed to help facilitate the establishment of an integrated eco-friendly disease management system for growers of Zamioculcas zamiifolia. Different media was also evaluated to mass produce the Trichoderma isolate. The media evaluated in this study included the solid substrates barley seeds, paddy, cow pea, maize and sorghum and semi solid, liquid substrates such as potato dextrose, rice extract, paddy extracts, respectively. Although mycelial growth was fastest in barley and paddy media. And the highest yield of spores of the Trichoderma isolate was observed 7 days after inoculation in Barley and Paddy media.
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