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Articles by V. Priyo Bintoro
Total Records ( 3 ) for V. Priyo Bintoro
  Dwi Novrina Nawangsari , Ahmad Ni`matullah Al-Baarri , Sri Mulyani , Anang Mohamad Legowo and V. Priyo Bintoro
  Lactoperoxidase (LPO) could be simply obtained from whey through immobilization using a cation exchange resin of SP Sepharose. LPO received high attention since the antimicrobial properties of LPO system (LPOS) that are consisted of LPO, SCN¯ and H2O2 was able to generate OSCN¯ for strong antimicrobial agents. This study was done to analyze the immobilization efficiency of LPO onto two types of sepharose: SP-Sepharose Fast Flow (SPFF) and SP-Sepharose Big Beads (SPBB). The remaining of LPO’s activity (%) against storage solution was also observed. The whey was obtained from bovine skimmed milk that was coagulated using rennet and acid lactic. The LPO was obtained from whey using SPFF. To analyze the resistence of enzyme activity, the immobilized LPO was stored in pure water, phosphate buffer, milk and whey at 10°C. The activity of LPO was monitored for 10 days. The result indicates that the LPO could be purified from whey and single band has been detected using electrophoresis method. The obtained LPO (35 U mL-1) was attached onto SPFF and SPBB. Maximum immobilization efficiency has been achieved by 0.6 g SP-FF and 0.9 g SP-BB. LPO activity of the immobilized LPO were able to be kept until 5 days when it was stored in whey. Other storage solution remained various LPO activity during storage. This study concluded that IE of LPO attached onto sepharoses might be reached variously depended on the sepharose type and whey as LPO source might be used for maintaining LPO activity.
  Rasbawati , Bambang Dwiloka , Ahmad Ni`matullah Al-Baarri , Anang M. Legowo and V. Priyo Bintoro
  Dangke is the Indonesian cheese produced from bovine milk using latex from young papaya to coagulate casein. Dangke is generally consumed by Indonesian people located in South Sulawesi Province. In line with demand of Dangke, the preservation is needed. Since, there was no literature which was found about preservation of Dangke, this study is aimed at knowing the quality of Dangke based on total bacteria and pH value stored in antimicrobial agent of lactoferrin and lactoperoxidase system from bovine whey, aquadest and phospate buffer at ambient temperature for 12 h. The lactoferrin, lactoperoxidase and whey were obtained from bovine milk and purified using ion exchange chromatography method. The result of the study showed that lactoperoxidase system provide remarkable effect of decreasing total bacteria from 8 log CFU mL-1 to 5 log CFU mL-1 while other storage solutions have no antimicrobial activity against bacteria in Dangke. The pH value of Dangke was stable when stored in lactoferrin and lactoperoxidase system. Since, both of these preservatives could be categorized as safe, the application in Dangke may open the alternative method to store Dangke.
  Kurniawan Sinaga , Anang M. Legowo , V. Priyo Bintoro , Edjeng Suprijatna and Yoyok B. Pramono
  Duck’s leg and breast of Indonesian native duck were analized in laboratory to determine polycyclic aromatic hydrocarbon (PAH) such as naphthalene, acenaphtene, phenentrene, flourantene, pyrene, benzoantrasene and perylene. The result showed that naphthalene, flourantene, pyrene, benzoantrasene and perylene in leg’s duck was higher than breast duck. Total fat of legs (5.87%) were higher than breast (3.31%). This result showed that total fat of raw duck meat influence PAH formation. Antioxidant activity of leg was higher than breast’s duck (8.79% : 5.1%). This result showed that endogenous antioxidant did not able to prevent PAH formation.
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