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Articles by Ustadi
Total Records ( 3 ) for Ustadi
  Amir Husni , Ustadi and Hendra Wijaya
  Inhibition efforts for quality deterioration in fish can be performed using any preservatives. Preservatives that used must be sourced from natural ingredients, in order to minimize the effects of hazardous chemicals when using synthetic materials. One of natural preservatives from marine is the extract of Gracilaria sp. The fresh red tilapia were prepared to form the fillets and then soaked for 30 min in Gracilaria sp. extract solution with a concentration of 0, 0.5, 1, 1.5 and 2% then drained for 15 min. Red tilapia fillets stored at low temperature (6°C) for 10 days with an interval of observation every 2 days. Observations made on the organoleptic, measurement of pH, Total Volatile Bases (TVB) and Total Plate Count (TPC). Based on the value of pH, TVB and TPC, the extract of Gracilaria sp. it enabled to extend the storage life of the fillet from 6-10th day. Based on organoleptic, extract of Gracilaria sp. enabled to maintain the parameters of appearance, texture and smell of red tilapia fillet from 4-6th day of storage. The use of extracts of Gracilaria sp. 2% gave the best results based on the parameters of odor, pH, TVB and TPC.
  Amir Husni , Renita Wijayanti and Ustadi
  The objective of this study was to determine the potential of polyphenols and phlorotannins extract from Padina pavonica in inhibiting α-amylase and α-glucosidase. Total phenolic content of P. pavonica was 17.63±0.25 μg GAE/mg extract and total phlorotannins content was 1.94±0.19 μg PGE/mg extract. The results showed that polyphenols (IC50 = 0.36±0.05 mg mL-1) and phlorotannins (IC50 = 0.25±0.02 mg mL-1) can inhibit α-amylase, but lower than acarbose (IC50 = 0.06±0.00 mg mL-1). Polyphenols (IC50 = 26.57±2.08 mg mL-1) and phlorotannins (IC50 = 34.40±5.93 mg mL-1) can also inhibit α-glucosidase but also lower than acarbose (IC50 = 1.74±0.25 mg mL-1). Both polyphenol and phlorotannin extracts from P. pavonica have higher inhibitory activity of α-amylase than α-glucosidase. There were high correlations between total phenolic and phlorotannin contents with inhibition activity of α-amylase and α-glucosidase.
  Amir Husni , Dwi Purwanti and Ustadi
  The objective of this study was to evaluate the potential effect of sodium alginate from Sargassum crassifolium on glucose level and lipid profile in streptozotocin-induced diabetes rats. Sodium alginate extract of S. crassifolium 200, 400 and 600 mg kg–1 was administered orally to streptozotocin-induced diabetes rats, once daily for 15 days. The result obtained were then compared with normal control (non-diabetic+normal saline 0.9% p.o), negative control (diabetes+CMC-Na 0.5% p.o) and positive control (diabetic+glibenclamide 5 mg kg–1 p.o). The glucose level, lipid profile and body weight were measured on normal condition (baseline) on 0, 5th, 10th, 15th day and pancreatic histopathological study were done on 15th day. Sodium alginate extract of S. crassifolium had yield 23.82% db, viscosity of 521 cps and a water content of 16.18% db. Sodium alginate 600 mg kg–1 significantly reduce level preprandial glucose, postprandial glucose and total cholesterol compared negative control and did not have significant difference with positive control. Levels of triglycerides and LDL-c throughout the treatment groups had significant differences with the negative control. Meanwhile, the levels of HDL-c throughout the treatment group did not have significant differences. Necrosis was found in all streptozotocin-induced rats. The higher alginate doses given, getting better lower the levels of glucose and total cholesterol.
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