Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Usama Ahmed Fahmy
Total Records ( 3 ) for Usama Ahmed Fahmy
  Usama Ahmed Fahmy and Bader Mubarak Aljaeid
  Background and Objective: Erectile dysfunction (ED) is a common condition, especially in diabetic males. Alpha-lipoic acid (ALA) can protect endothelial cells from oxidative stress damage. The purpose of this study was to find a new treatment for ED in diabetic patients via combined ALA with tadalafil (TFL) in a transdermal patch to enhance the skin permeability of TFL and protect epithelia cells lined penile vessels from the oxidative stress induced by hyperglycemia. Materials and Methods: A solubility study was carried out for TFL and ALA, followed by nine formulae with different ratios of oil, surfactant and co-surfactant. TFL released from the formulated patch was compared with a raw TFL patch and afterward, permeation of the formulated TFL was imaged. The difference between the treatments was estimated using one way-analysis of variance test. Results: Anise oil and Tween 20 showed highest solubility for both drugs, while polyethylene glycol (PEG 200) solubilized 0.49±0.1 and 7.8±1.2 mg mL–1 of TFL and ALA, respectively, Formula A4, achieved the lowest globular size (134.3±12.3 nm) according to ternary phase diagram. TFL released was enhanced significantly by about 2.83-fold in the transdermal patch in comparison with raw TFL after 12 h, images clearly confirm the efficacy of penetration of the formula within the skin layers. According to cell viability (EA. hy926) data to assess the role of ALA in improvement of resistance of endothelial cells to oxidative stress, ALA increased cell survival by about 1.3-fold. Conclusion: The combination of ALA and TFL in the form of a self-nano emulsion drug delivery system as a transdermal patch could be a successful strategy for treatment of ED in diabetic patients.
  Usama Ahmed Fahmy , Osama Abdelhakim Aly Ahmed and Nabil Abdulhafiz Alhakamy
  Background and Objective: Alendronate sodium (ALS) is a member of the class bisphosphonates used to treat osteoporosis. Recent reports suggested that ALS may induce cell death and inhibit the proliferation of estrogen-sensitive MCF-7 breast cancer cells. The aim of this work was the combination of ALS and trans-activating regulatory protein (TAT) loaded nanostructured lipid carriers (NLCs) to potentiate ALS cytotoxicity against MCF-7 breast cancer cells. Materials and Methods: The ALS and TAT were prepared and loaded in NLCs. The formed NLCs were characterized for entrapment efficiency (%) (EE %), particle size and zeta potential. Cell viability was also carried out for the prepared NLCs. Results: The results revealed that the prepared NLCs were spherical, with particle sizes of 285.2±14.1 nm and polydispersity index of 0.51. Zeta potential of ALS-TAT-NLCs was +21.1±3.4 mV. The ALS EE percentage of the prepared NLCs was 12.2±1.1%. At concentration of 10 μg mL–1, the investigated formulations showed cell survival percentage of 6.86, 20.34, 97.53 and 91.55% for ALS-TAT NLCs, raw ALS, raw TAT and plain NLCs, respectively. The IC50 values for the treatments were estimated as follows: ALS-TAT NLCs, 1.98±0.17 μg mL–1, raw ALS, 2.77±0.36 μg mL–1, raw TAT, 1066.67±21.22 μg mL–1 and plain NLCs, 10816.2±112.32 μg mL–1. Conclusion: The combination of ALS with TAT protein loaded NLCs offered a dual mechanism of ALS activity potentiation and a promising candidate for breast cancer therapy.
  Mohamed Fouad Radwan , Mohamed Ahmed El- Moselhy , Usama Ahmed Fahmy and Bader Mubarak Aljaeid
  Background and Objective: Erectile dysfunction (ED) is a common disease in diabetic patients. Alprostadil (Prostaglandin E1, PGE1) has shown higher success rates compared with phosphodiesterase type 5 inhibitors (PDE-5) in the treatment of ED. The combined formula of PGE1 with D-tocopheryl polyethylene glycol succinate (TPGS) was aimed to find a novel treatment for diabetic patients suffering from ED via protecting epithelial cells that lined penile vessels from the oxidative stress. Materials and Methods: PGE1-TPGS was prepared either with labrasol, polyethylene glycol 200 (PEG 200) or a mixture of labrasol and PEG 200, F1, F2 and F3 respectively. The prepared formulations were characterized for morphology and zeta potential and then were added to 1.5% of hydroxypropyl methyl cellulose solution to form topical hydrogel. Drug release study for all investigated formulations was conducted in comparison with raw-PGE1 and tested in endothelial cells (EA. hy926) under stress condition. Results: The obtained data revealed that PGE1-TPGS prepared with labrasol achieved the highest particle size while, PGE1-TPGS formula of PEG 200 and labrasol mixture showed the smallest particle size. Transmission electron microscope (TEM) images of the prepared formulations showed spherical shape micelles. Oxidative stress investigation showed a dramatic improvement in epithelial cell survival that attenuated the permeation enhancer’s cytotoxicity. F1, F2 and F3 treatments showed improved cell viability when compared with blank formulations. At concentration 10 μg mL–1, results showed that the investigated formulations improved cell viability from 9.13% (F1 blank), 7.55% (F2 blank) and 8.82% (F3 blank) to 90.52, 91.27 and 93.94% for F1, F2 and F3 formulations, respectively. Conclusion: The study revealed that combination of PGE1 with TPGS micelles formula loaded topical gel could be a successful strategy for the treatment of ED in diabetic patients.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility