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Articles by Ugwueze Mercy
Total Records ( 1 ) for Ugwueze Mercy
  Adonu Cyril , Gugu Thaddeus , Onyi Patrick , Onwusoba Restus , Ugwueze Mercy , Ugwu Malachy and Esimone Charles
  Plasmid analysis is an important method used in the determination and characterization of antibiotic drug resistance traits in procaryotes. This study is aimed at investigating the prevalence and plasmid profile of fluoroquinolone-resistant Escherichia coli isolated from some domestic livestocks in Enugu State, Nigeria. A total of 559 E. coli isolates from three domestic livestocks comprising pig, cattle and chicken in seven health districts of Enugu State, Nigeria were screened for antibiotic susceptibility and plasmid profiles. The isolates were tested against 9 antibiotics using the agar disc diffusion method while plasmid DNA was extracted using the alkaline SDS method and separated by agarose gel electrophoresis. About 34 of 559 (6.0%) of the isolates were found to be Fluoroquinolone-Resistant E. coli (FQREC). The prevalence of fluoroquinolone resistance among the E. coli isolates from the animals tested were: pig (5.7, 6.1, 5.7 and 7.8%), cattle (0, 0, 0 and 0%) and chicken (13.6, 14.3, 11.6and 17.7%) for ciprofloxacin, ofloxacin, levofloxacin and pefloxacin, respectively. Very high resistance levels (>63 %) were detected against amoxycillin, erythromycin and doxycycline while gentamicin and ceftriaxone recorded the least resistance levels of 8.3 and 5.2%, respectively against the isolates. Plasmids of different sizes were detected in the isolates. Out of the 24 plasmids detected in the FQREC isolates, 9 different profiles were recorded; 3 in pigs and 6 in chickens. Isolates with high multi-drug resistance profiles were found to possess multiple plasmids with large sizes in the range of 2026-23130 bp. The plasmids were cured to the range of 40-80% depending on the source of the isolates thus, confirming the contribution of plasmid in mediating fluoroquinolone resistance in animal FQREC isolates. There was high prevalence of FQREC isolates in the studied districts and may constitute a potential reservoir of resistance plasmids that could be transferred to pathogenic bacteria.
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