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Articles by Trivadila
Total Records ( 2 ) for Trivadila
  Dyah Iswantini , Nadinah , Latifah Kosim Darusman and Trivadila
  Apium graveolens, one of the traditional medicinal plants, has a potential as anti-gout. We have reported that flavonoid of A. graveolens could inhibit activity of xanthine oxidase enzyme up to 85.44%. The aim of the research was to investigate the type of inhibition kinetic of A. graveolens ethanol crude extract and its fraction inhibition kinetic, also to determine the active compound. The result of the research showed that the yield of A. graveolens ethanol crude extract was 10.40 % (LC50 1968.19 mg L-1) with the inhibition activity was 6.04% until 74.01% (100-2000 mg L-1). Inhibition kinetic of 1500 mg L-1 crude extract caused increased KM (0.10 mM) and unchanged Vmax. Based on these data, the type of inhibition was competitive. Purification of crude extract resulted 7 fractions and the highest activity was achieved by fraction 6 (inhibition activity was 85.08%). The purification of crude extract caused the increasing of inhibition activity effect. Inhibition kinetic of fraction 6 (150 mg L-1) caused increase KM (0.30 mM) and unchanged of Vmax. Based on that, the type of inhibition was competitive. Purification of fraction 6 resulted 6 fraction and the highest activity was achieved by fraction 5 (inhibition activity was 88.41%). Based on analysis of LCMS and NMR, the active compound of A. graveolens extract (fraction 5) were potential to inhibit the activity of xanthine oxidase, the active compound was 5, 7-dihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one and 2, 3-dihydro-6-hidroxy-5-benzofuran carboxylic acid.
  Dyah Iswantini , Novik Nurhidayat , Trivadila and Okik Widiyatmoko
  Determination of uric acid concentration in human urine and blood is needed to diagnose several diseases, especially the occurrence of kidney disease in gout patients. Therefore, it is needed to develop a simple and inexpensive method for uric acid detection. The purpose of the research was to observe the use of Indonesian microbe that was immobilized on natural zeolite as a source of uricase for uric acid biosensor. Selection of mediators and determination of optimum condition measurement, the stability and kinetic properties of L. plantarum uricase were performed using carbon paste electrode. Cyclic voltammetry was employed to investigate the catalytic behavior of the biosensor. The result indicated that the best mediator for measurement of L. plantarum uricase activity was Qo (2,3-dimethoxy-5-methyl-1,4 benzoquinone). Optimum conditions for immobilization of L. plantarum uricase on zeolite were obtained at pH 7.6, with temperature of 28°C, using uric acid concentration of 0.015 mM and zeolite mass at 135 mg KM and VMax of L. plantarum uricase obtained from Lineweaver-burk equation for the immobilization uricase on zeolite were 8.6728x10-4 mM and 6.3052 mM, respectively. KM value of L. plantarum uricase directly immobilized onto the electrode surface was smaller than KM value of L. plantarum uricase immobilized on zeolite. The smaller KM value shows the higher affinity toward the substrate. The Electrode when kept at 10°C was stable until 6 days, however the immobilized electrode on zeolite was stable until 18 days. Therefore, Indonesian L. plantarum could be used as a uric acid biosensor.
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