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Articles by Tri Baskoro Tunggul Satoto
Total Records ( 2 ) for Tri Baskoro Tunggul Satoto
  Dwi Sutiningsih , Mustofa , Tri Baskoro Tunggul Satoto and Edhi Martono
  Background and Objectives: Aedes aegypti Linnaeus is a mosquito species that plays an important role as a vector of diseases in tropical and subtropical regions. Aedes aegypti (L.) resistance against temephos and pyrethroid has been widely reported in several countries including Indonesia. This study aimed to examine the neurotoxic mechanism of bruceine A biolarvicide and to determine the inhibitory activity of the enzyme acetylcholinesterase and gene Voltage-Gated Sodium Channel (VGSC) on A. aegypti (L.) larvae. Materials and Methods: Bruceine A was obtained by extraction and isolation of Makassar fruit’s seeds. Test of inhibition of acetylcholinesterase activity was based on Ellman method. Voltage-Gated Sodium Channel gene inhibitor was tested using PCR. The statistical analysis of inhibition of the enzyme acetylcholinesterase was performed by using Kruskal Wallis test followed by Mann Whitney. The data from observation on behavior response and VGSC gene were descriptively analyzed. Results: Bruceine A could inhibit the action of the enzyme acetylcholinesterase as much as 45.083±0.003%, with symptoms of a neurotoxic that is excitation, convulsions, tremors and paralysis. The result of fragment amplification of VGSC gene and electrophoresis on agarose gel 2% generated in a specific band with the size of the fragment bands of 336 bp on samples of larvae that were still sensitive to bruceine A and cypermethrin. Conclusion: Bruceine A isolated from the seeds of Brucea javanica (L.) Merr. has been demonstrated as biolarvicide against larvae of A. aegypti (L.), through its neurotoxic properties. The mechanism of neurotoxicity is mediated through inhibition of enzyme acetylcholinesterase and VGSC gene.
  Tri Baskoro Tunggul Satoto , Tridjoko Hadianto , Nika Istiyana , Siti Badriyah and Ajib Diptyanusa
  Background and Objective: Mosquito resistance against different varieties of chemical pesticide had been frequently reported. Therefore, a substitute using natural substances is necessary. One substance that has the properties of a natural insecticide is plant-derived Tagetes essential oil (Tagetes erecta L.). Preliminary study to assess the mosquitocidal activity of 100% Tagetes erecta L. essential oil against Aedes aegypti and Culex quinquefasciatus mosquitoes, contrasted with 13.16 g L–1 prallethrin as the gold standard. Materials and Methods: Leaves of Tagetes erecta L. were distilled by using water and steam distillation method to obtain the essential oil. No synthetic compounds were added to the production process to acquire concentration of 100% Tagetes erecta L. essential oil. Mosquitoes were divided into 3 groups: Group A (aquadest), Group B (100% Tagetes oil) and Group C (13.16 g L–1 prallethrin). Trials were conducted using 25 mosquitoes aged 2-5 days introduced into a test chamber and exposed to the substance by using electric liquid vaporizer according to the test group. Test replications were done 3 times. Knockdown time (KT50 and KT90) was observed and mosquito mortality was confirmed after 24 h. Data analysis was performed by using Probit analysis (95% CI). Results: In 100% Tagetes erecta L. essential oil treatment group, KT50 was 140 min and KT90 was 187 min in Aedes aegypti, whereas in Culex quinquefasciatus, KT50 was 91 min and KT90 was 151 min. In 13.16 g L–1 prallethrin treatment group, KT50 was 64 min and KT90 was 109 min in Aedes aegypti, whereas in Culex quinquefasciatus, KT50 was 83 min and KT90 was 137 min. There were 1 Aedes aegypti and 1 Culex quinquefasciatus mosquitoes found dead in aquadest treatment group after 24 h of observation. Conclusion: Leaves extract of 100% Tagetes erecta L. showed mosquitocidal activity against Aedes aegypti and Culex quinquefasciatus mosquitoes, yet it was not superior compared to 13.16 g L–1 prallethrin.
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