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Articles by Tony Hadibarata
Total Records ( 6 ) for Tony Hadibarata
  Topik Hidayat , Mohd. Aszuan Abdul Samat , Muhamad Atiq bin Elias and Tony Hadibarata
  Index for water quality is solely based upon the conditions of physical and chemical which provide quantitative data on the presence and level of aquatic pollution. However, the parameters for the assessment do not represent the environmental stress and ecological health of the river which affect the microbial diversity. In this study, we introduced a new approach of assessing river water from metagenomic view by constructing phenetic tree which show relation between microbial community and the level of pollution. Four river waters, of which one river is categorised as unpolluted water, located around Johor Bahru (Malaysia) were examined and compared. Genomic DNA of these uncultured community of microorganisms was directly extracted and 16S rRNA gene was amplified by PCR using a set of primer pairs to generate cloned libraries. In total 24 isolates which are consisted of 18 isolates from polluted rivers and six from unpolluted one, were sequenced. Along these sequences, six of 16S rRNA sequences of colliform bacteria obtained from genebank were placed in Operational Taxonomic Units (OTUs). Phenetic analysis revealed that the river water used in this study were classified into two groups, representing the polluted and unpolluted. Since the tree can clearly distinct unpolluted river water from that of polluted one, we tried to develop a putative sequences motif for both condition. In practical purpose, the sequences motif can be used to screen river water quality in certain river water environment.
  Tony Hadibarata
  The aim of this study is to evaluate amaranth textile dye decolorization by white-rot fungus Pleurotus eryngi F019 isolated from tropical rain forest. The isolate was able to decolorize an azo dye in wide range (up to 200 mg), at temperature 25°C. The results revealed that the removal azo dye amaranth was rapid at the initial incubation period (15 days) and became slowly with the time (30 days). The maximum decolorization was observed after 30 days incubation. Addition of Cu2+, Mg2+, Mn2+ gave the positive results while decolorization was inhibited by addition of Fe2+. Metal ion also affected the level of enzyme production during decolorization of amaranth. Induction in the activity of laccase and lignin peroxidase was observed during decolorization of amaranth in the culture, which represented their important role in biotransformation. The biodegradation of amaranth dye was monitored by UV-Vis spectrometer and gas chromatography. These promising results suggest the application of Pleurotus eryngi F019 to treat dye containing wastewater having higher concentrations of metals.
  Tony Hadibarata , Sanro Tachibana and Kazutaka Itoh
  Microbial degradation of Phenanthrene with several fungi screened from nature was conducted to select fungi for the bioremediation of Phenanthrene. Thrichoderma sp. S019, a fungus collected from soil, had the highest rate of degradation on the agar medium containing Phenanthrene. Maximal degradation (72%) was obtained when Trichoderma sp. S019 was incubated for 30 days after the addition of 0.1 mM of Phenanthrene to the liquid medium. Furthermore, the degradation of Phenanthrene was affected by the addition of a carbon source, the addition of a nitrogen source and agitation. Also, 1,2-Dioxygenase and 2,3-Dioxygenase were produced by Trichoderma sp. S019 in a liquid medium. These enzymes play an important role in the metabolism of substrates, revealing a high stereoselectivity for initial dioxygenase and enzymatic hydration since the K-region of phenanthrene was the major site of metabolism. Phenanthrene was indeed degraded by Trichoderma sp. S019 because 1-Hydroxy-2-naphthoic acid, Salicyaldehyde, Salicylic acid and Catechol, considered to be the intermediates in the bioremediation of Phenanthrene, were detected among the reaction products.
  Tony Hadibarata , Sanro Tachibana and Kazutaka Itoh
  The degradation of n-eicosane by Trichoderma sp. S019, a fungus collected from soil with extensive degradative ability on an agar medium containing n-eicosane, was demonstrated in liquid medium and in soil. Maximal degradation (77%) was obtained when Trichoderma sp. S019 was incubated for 30 days after the addition of 0.1 mM of n-eicosane to the liquid medium while the highest rate of degradation (40%) was obtained in soil with the addition of 1.5% Trichoderma sp. S019. Furthermore, the degradation of n-eicosane was affected by the addition of a carbon source, the addition of a nitrogen source and agitation. n-Eicosane was indeed degraded by Trichoderma sp. S019 because nonadecanoic acid, n-octadecane, hexadecanoic acid, oleic acid and stearic acid, considered to be the intermediates in the biodegradation of n-eicosane, were detected among the reaction products.
  Agung Endro Nugroho , Ichwan Ridwan Rais , Iwan Setiawan , Pramita Yuli Pratiwi , Tony Hadibarata , Maulana Tegar and Suwidjiyo Pramono
  Andrographis paniculata (Burm. f.) Nees is a plant that originates from India and grows widely to Southeast which used for several purposes mainly as treatment of diabetes mellitus so the aim of this study was evaluate andrographolide for its pancreatic effect in neonatal streptozotocin (STZ)-induced diabetic rats, a model of type 2 diabetic rats. Diabetic condition was induced with an intraperitoneal injection of 90 mg kg-1 streptozotocin in two-day-old rats. After three months, the neonatal STZ-induced diabetic rats were treated with andrographolide or andrographolide-enriched extract of A. paniculata (AEEAP) for 8 consecutive days. Pancreatic effect was evaluated by estimating mainly the preprandial and postprandial blood glucose levels and other parameters such as morphology of pancreatic islet, beta cells density and morphology and immunohistochemically pancreatic insulin. Andrographolide significantly (p<0.05) decreased the levels of blood glucose and improved diabetic rat islet and beta cells. However, AEEAP exhibited moderate hypoglycaemic effects on the blood glucose levels. Moderate changes in beta cells were observed after AEEAP treatment. They could restore decreasing of pancreatic insulin contents. Based on these results andrographolide and AEEAP exhibited pancreatic actions in neonatal STZ-induced diabetic rats. The activity of andrographolide was more effective than this of AEEAP.
  Rita Sundari , Tony Hadibarata , Lee Yook Heng and Musa Ahmad
  Formaldehyde is a known carcinogen which may cause cancer when accumulated in the body. This study showed the results of a formaldehyde biosensor which was fabricated by nanogold doping in a poly-2-hydroxy ethyl methacrylate (p-HEMA) membrane. The biocatalysts used for the biosensor were 1.0% ferrocene mediator and alcohol oxidase which was then deposited on a carbon screen-printed electrode. 2,2-Dimethoxy-2-phenyl-acetophenone (DMPP) was applied to the membrane as a polymerization agent. The amperometric method was employed with a phosphate buffer solution (pH = 7.2). The optimum potential was selected to be 0.3 V which obtained good linear calibration (R2 = 0.99) for a range of 0.02-0.16 mM formaldehyde (n = 4). The RSD (Relative Standard Deviation) and LOD (Limit of Detection) were found to be 5.62% and 0.007 mM formaldehyde, respectively. The fabricated biosensor successfully detected formaldehyde in selected fresh foodstuffs (tauhu, meatballs, shrimp and dried and wet fish) and the results were well correlated with the NASH standard method.
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