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Articles by Tongao Yang
Total Records ( 1 ) for Tongao Yang
  Yongfeng Sun , Tongao Yang , Jingtao Hu , Zhe Hao , Yujian Sui , Yingying Fu , Lu Chen , Haiyang Zhu and Wei Wu
  The muscular tissue of breast was dissected from 8 weeks old Jilin White goose in the present study. The big fragment PCR Method was used to amplify double-strand cDNA based on the SMART techniques for construction of a full-length cDNA library. After digestion with restriction endonuclease Sfi |, a modified vector of pBluescript II SK-plasmid with the adaptors containing Sfi |A and Sfi |B sites was used to recombine with the cDNA products amplified. The recombinants were cloned by transformation into competent Escherichia coli DH2α. A plasmid cDNA library with goose muscle was constructed. The results showed that the titer of the cDNA library was 1.01x106 pfu mL-1 and the percentage of recombinant clones was 97%. The length of most cDNA inserted was between 0.25 and 1.6 kb identified by gel electrophoresis after cDNA PCR amplification. The unigene ratio was 66.7% and the percentage of complete cDNA sequences was 80% by estimating from the 24 clones sequenced randomly. It is helpful to study muscle development of goose at molecular level in the future.
 
 
 
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