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| Articles
by
Tong Qin |
Total Records (
2 ) for
Tong Qin |
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Huabin Zhu
,
Bo Lin
,
Jun Chen
,
Haisheng Hao
,
Xueming Zhao
,
Shujing Li
,
Weihua Du
,
Tong Qin
,
Yan Liu
and
Dong Wang
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A rapid and simple PCR sex identification of embryo is very important for bovine embryo transferring. Many sex identification methods using duplex PCR were established according to Sry gene. But the identification process was affected greatly by more primers interaction. In order to decrease the interference from more primers, researchers explored a simple and rapid PCR Method. The sequences of Amelogenin alleles located at both sex chromosomes were downloaded from GenBank. A pair of sex specific primers was designed to span the 63 bp longer insertion sequence in X chromosome. Bovine samples of blood, fibroblasts and demi-embryos were sexed with these primers. Two-temperature PCR cycling program was used in which the extension step was deleted while the denaturizing and annealing steps were shortened to 1 sec. The results shown ideal identification were obtained and observable amplification were also obtained using even single fibroblast. About 20 bovine embryos were identified by this PCR cycling program and 15 embryos (9 females and 6 males) were transferred. The sexing results were confirmed by the anatomically proven sex after parturition, respectively. The comparison of amplification results between blood samples of bovine and human shows the excellent, specificity to bovine. Thus, a simple, rapid and effective PCR sex identification method was established. |
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Tong Qin
,
Haoyu Wang
,
Dengpan Bu
,
Haisheng Hao
,
Dong Wang
,
Weihua Du
and
Huabin Zhu
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The objective of this study was to evaluate the effect of
a corn straw or mixed forage diet on endocrine, metabolism and lactation performance
in periparturient Holstein cows. Twelve multiparous, periparturient Holstein
cows were randomly assigned to two groups and fed a corn straw or mixed forage
diet, respectively. The CS diet included 33.8% corn straw and the F:C ratio
[Dry Matter (DM)] was 60:40. The MF diet included 3.7% Chinese wildrye, 28.4%
alfalfa hay and 26.5% corn silage, the F:C ratio (DM) was 40:60. All cows were
fed from weeks 3-8 and Body Weight (BW), Body Condition Score (BCS) and Dry
Matter Intake (DMI) were recorded. Milk protein, fat, lactose and Somatic Cell
Count (SCC) were determined twice weekly. Metabolite and hormone analyses of
blood were made weekly. Results showed that dietary treatments had no detectable
effects on BW, BCS, DMI and blood hormones measured. From the 6 weeks of lactation,
cows fed MF diet produced more milk (p<0.05) and tended to produce more milk
fat (p = 0.07) and protein (p = 0.10) compared with cows fed CS diet. The proportions
of milk fat, protein and lactose (%) did not differ between the two dietary
treatments (p>0.05). In addition, the milk SCC in the CS group was significantly
higher (p = 0.02) than the MF group. Cows fed MF diet experienced more severe
Negative Energy Balance (NEB) and had higher concentrations of β-Hydroxy
Butyric Acid (BHBA) and Non-Esterified Fatty Acid (NEFA) than cows fed CS diet.
Collectively, these results suggest that cows fed MF diet improve lactation
performance of periparturient cows but this dietary treatment may have an adverse
effect on NEB of periparturient cows. |
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