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Articles by Tian-Hua Huang
Total Records ( 2 ) for Tian-Hua Huang
  Halima Hassan Salem , Tian-Hua Huang , Bahy Ahmed Ali and Qing-Dong Xie
  Random Amplified Polymorphic DNA (RAPD) is a method of producing a biochemical fingerprint of a particular species. This study involved the isolation of DNA from three Bacillus thuringiensis (B. thuringiensis) [B. thuringiensis tenebrionis (tt4) and B. thuringiensis israelensis (i977)] as reference strains and new isolate B. thuringiensis strain (66) using the DNeasy Mini Kit (Qiagen, Inc. protocol). Two Escherichia coli (E. coli BL21 and E. coli C1a) DNAs are provided as components of RAPD Analysis Kit. RAPD analysis was performed using Ready-To-GoTM Analysis Beads and nine primers. Bands were then analyzed using a binary system and a phylogenetic tree created. The five strains segregated into two major clusters. The maximum linkage distance between the two major clusters was 103 units. First cluster consisted of two E. coli strains and the second cluster consisted of the three B. thuringiensis strains based on genetic similarity in their RAPD profiles. The B. thuringiensis reference strains were located in subcluster showing high similarity. Genetic similarities estimated as band sharing were calculated and all comparisons among bacterial strains have been done. The results showed that RAPD-PCR is powerful method for differentiation between B. thuringiensis and E. coli.
  Halima Hassan Salem , Tian-Hua Huang , Bahy Ahmed Ali and Qing-Dong Xie
  The aim of this study was to evaluate the genetic similarity and phylogenetic relationship among four Bacillus thuringiensis subspecies (new isolate and three reference strains). Random amplified polymorphic DNA (RAPD) technique was used in this study. Based on RAPD pattern obtained with total of seventeen random primers, the similarity index was calculated for each primer separately and the average for all primers was carried out with each comparison. Moreover, the scored band data was subjected to cluster analysis using Statistica/W5.0 Software package. Based on RAPD data obtained with all primers, the average of genetic similarities between the new isolate (66) and ACCC 10061, tt4 and i977 was 42.47; 51.10; and 45.90, respectively. The four strains are grouped into two major clusters A and B. Cluster A consisted of B. thuringiensis Berliner (ACCC 10061) strain and cluster B consisted of the three B. thuringiensis (66, tt4 and i977) strains. RAPD-PCR could be used extensively to study phylogenetic relationship among B. thuringiensis strains from different subspecies as well as for identification new isolate bacterial strains.
 
 
 
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