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Articles by Thnaian Althnaian
Total Records ( 4 ) for Thnaian Althnaian
  Thnaian Althnaian , Ibrahim Albokhadaim and Sabry M. El-Bahr
  The stimulation of oxidative stress and lipid peroxidation of liver tissues by AFB1 has been extensively investigated. However, the effect of AFB1 on antioxidant enzyme activities and gene expression in testis has not been completely elucidated. The current study investigated the histopathological picture and activities in addition to gene expression of antioxidant enzymes in testis of rats intoxicated with AFB1. Twenty rats were divided into two equal groups. Rats in the first group served as control whereas, rats in the second group received single i.p. dose of AFB1 (3 mg kg–1 b.wt.). Testicular damage and oxidative stress were evident in AFB1-intoxicated rats as indicated by a significant increase in thiobarbituric acid reactive substances (TBARS), reduction of reduced glutathione (GSH) concentration, reduction in the activities of antioxidant enzymes namely catalase (CAT), total superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-s-transferase (GST) and down-regulation of gene expression of these antioxidant enzymes compare to control. Testicular sections of rats intoxicated with AFB1 showed mild testicular degeneration of some seminiferous tubules manifested by reduced numbers of spermatogenic cells and associated with buckling of the seminiferous basement membranes. In conclusion, testicular injury, high lipid peroxidation, low GSH concentration and inactivation and down-regulation of gene expression of antioxidant enzymes are involved during AFB1 toxicity in rats.
  Thnaian Althnaian , Ibrahim Albokhadaim and Sabry M. El-Bahr
  Background and Objectives: Role of black cumin seeds (Nigella sativa) in enhancing gene expressions of catalase (CAT), glutathione-s-transferase (GST) and B-cell CLL/lymphoma 2 (Bcl-2) in diabetic rats has not investigated so far. Furthermore, sensitive technique is required to confirm its overexpression to superoxide dismutase (SOD), glutathione peroxidase (GPX) and insulin like growth factor-1 (IGF-1). This study was aimed to investigate the effect of black seed on gene expression of CAT, GST and Bcl-2 and also examine its expression to SOD, GPX and IGF-1 by high sensitive RT-PCR in diabetic rats. Materials and Methods: Eight non-diabetic albino rats assigned as control (group I). Sixteen rats rendered diabetic by streptozotocin (STZ; 60 mg kg1 b.wt.). Diabetic rats divided into group II and group III (eight rats in each). Rats in group II were untreated diabetic group while rats of group III were diabetic and treated with Black-cumin seed (2 g kg1 b.wt.) for 6 weeks. Results: Diabetes increased blood glucose level than control while black seed reduced the higher blood glucose in diabetic rats significantly. Except for IGF-1, diabetes induced significant increases in gene expressions of Bcl2, CAT, SOD, GPX and GST compare to control. Black seed increased expression of Bcl2, CAT, SOD, GPX, GST and IGF-1 genes compare to both control and diabetic untreated rats. Conclusion: The current study observed that the Nigella sativa showed overexpression to CAT, GST and Bcl2 genes and also to SOD, GPX and IGF-1 by higher quantitative PCR in STZ diabetic rats.
  Thnaian Althnaian
  Because of AFB1 effects on antioxidant enzyme activities and gene expression in kidneys have not been completely elucidated, the current study was designed to investigate the histopathological picture and activities and gene expression of antioxidant enzymes in kidneys of rats. Therefore, 20 rats were randomly divided into two equal groups. Rats in the first group served as control whereas rats in the second group received single i.p., dose of AFB1 (3 mg kg–1 b. wt.). Kidney damage and oxidative stress were observed in AFB1-intoxicated rats as indicated by a significant elevation in thiobarbituric acid reactive substances (TBARS), reduction of reduced glutathione (GSH) concentration, reduction in the activities of antioxidant enzymes namely catalase (CAT), total superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-s-transferase (GST) and down-regulation of gene expression of these antioxidant enzymes compare to control. Kidney sections of rats intoxicated with AFB1 showed swelling and vacuolar cytoplasm of distal tubules and desquamated distal tubular cells. In conclusion, AFB1 may exert its toxicity to kidney tissues by induction of lipid peroxidation and inactivation and down-regulation of gene expression of antioxidant enzymes.
  Ibrahim Albokhadaim , Thnaian Althnaian and S.M. El-Bahr
  In many species of birds, normal values for hematological and biochemical factors were measured and data base was established as their blood-profiles. In local Saudi chicken, measurement of serum biochemical values, which are important for diagnosis of clinical signs and symptoms when affected by diseases, are limited. Therefore, this study was carried out to investigate serum's biochemical values of local Saudi chicken. Therefore, forty chicks (1 month old; 20 males and 20 females) and another forty chicks (3 month old; 20 males and 20 females) were obtained from the farm of the Veterinary Research Station, King Faisal University, Al-Ahsa, Saudi Arabia and used as materials in the study. Blood samples were collected from all birds and the harvested sera were kept frozen at -20°C until the time of analysis. The present findings indicated no significant difference (p>0.05) of all examined biochemical parameters between male and female chickens or young and old birds. Interestingly, all investigated biochemical parameters in these chickens were lower than the recorded reference values of other birds except for glucose which exhibited higher values than the reference. The reported lower serum cholesterol and triacylglcerol concentrations might be reflected on their concentrations in meat and protecting human beings from atherosclerosis. Therefore, estimation of lipid profile in these birds is recommended for future study.
 
 
 
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