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Articles by Tayyab Husnain
Total Records ( 3 ) for Tayyab Husnain
  Tayyab Husnain , Tahira Fatima , Rafi-ul-Islam and Sheikh Riazuddin
  In order to develop an efficient protocol for inserting novel genes in chickpea plants, conditions of regeneration and transformation of tissues were optimized. In case of in vitro plant regeneration of Cicer arietinum variety 6153, the hypocotyl tissue showed somatic embryogenesis and regenerated shoots in two basal media with addition of NAA and BAP. Different auxins seemed to have different effects on the differentiation and maturation of embryos. The replacement of one auxin NAA with another IAA induced the germination of embryos. Elaborate rooting of regenerated shoots in two basal media containing IBA was obtained with a frequency of 80 percent. Conditions of biolistic transformation were also optimized for transient expression of marker gene using a homemade particle acceleration gun. The beta-glucuronidase gene was introduced into hypocotyl tissue for transient expression and upto 58 percent of hypocotyl showed transient expression. These results provide the basic information for the transformation of chickpea via somatic embryogenesis.
  Sultana Rasheed , Tahira Fatima , Khurram Bashir , Tayyab Husnain and Shiekh Riazuddin
  Seeds of three indica rice varieties B-370, B-2000 and Super basmati were sterilized and incubated on callus inducing medium (MS-Medium with 2 mgl-1 2,4-D) for 2, 4, 6 and 8 weeks and calli were obtained after somatic embryogenesis. After the incubation periods, calli were transferred to the regeneration medium. Regeneration efficiencies decreased from 98.5, 98 and 95% after two weeks incubation to 30, 34 and 24% after eight weeks incubation for B-370, Super basmati and B-2000 respectively. Some interesting patterns for adventitious root formation were also observed. Significant variation was observed within and among different cultivars and incubation periods for average number of tillers, average plant height, number of panicles per plant, panicle length and average yield per plant. Seven interesting somaclones were marked and scored for agronomic and physiochemical analysis. Physiochemical studies did not reveal any significant variation. Present studies clearly indicated that somaclonal variation can successfully be used for creating genetic variation for varietal improvement.
  Samina Noor , Tayyab Husnain and Sheikh Riazuddin
  A simple leaf tip assay was used for screening of putative transgenic plants expressing the hygromycin resistance gene (hph) or kanamycin resistance gene (nptll). Leaf tips were excised from in vitro or in vivo transgenic plants and cultured on MS medium without phytohormons containing the suitable concentrations of hygromycin and kanamycin. Leaf tips of plants transformed with the marker gene showed no significant effect of the appropriate drug for at least two weeks. While non-transgenic leaf tips had noticeable symptoms of bleaching, necrosis or browning after 3-4 days of selection. This method is simple, rapid and allows clear distinction between transformed and non-transformed plants both, in monocots and dicots.
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