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Articles by Taweerat Vichitsoonthonkul
Total Records ( 2 ) for Taweerat Vichitsoonthonkul
  Thalisa Yuwa-Amornpitak , Taweerat Vichitsoonthonkul , Morakot Tanticharoen , Supapon Cheevadhanarak and Sansnarak Ratchadawong
  Mycorrhizae are symbiotic association between specialized soil fungi and plants. Trees of Dipterocarpaceae have long been known for their ability to associate with ectomycorrhizal (ECM) fungi. Apart from modifying the lateral root system of their host, ECM fungi benefit their host plant in many ways, such as: protecting against root pathogens, providing mineral nutrients, increasing drought tolerance and enhancing seeding growth in the nursery. In this study, DNA from ectomycorrhizal roots was successfully amplified from 15 species in 6 genera of regular type. These included Anisoptera (1 species), Cotylelobium (1 species), Dipterocarpus (5 species), Hopea (3 species), Parashorea (1 species) and Shorea (5 species). Molecular techniques were used to identify the ECM. ITS (internal transcribed spacer) fragments located between the 18 S and 28 S rRNA genes in the nuclear genome were amplified and sequenced. The sequences of ITS were compared with sequences in the GenBank. The results revealed that the 33 mycobionts participating in the symbioses in ECM roots were in 8 families including Thelephoraceae (13), Russulaceae (6), Amanitaceae (1), Cortinariaceae (3), Sclerodermataceae (4), Agaricaceae (1), Pisolitaceae (3) and Boletaceae (2). The observed host specialization showed that these fungi could associate with more than one host plant species.
  Thalisa Yuwa-amornpitak , Taweerat Vichitsoonthonkul and Morakot Tanticharoen
  Dipterocarpoideae is a species-rich subfamily of Dipterocarpaceae. Dipterocarpaceae in Thailand is represented by 65 species in 8 genera. In recent years molecular taxonomist constructed a phylogenetic tree of dipterocarps found in South-East Asia. However, many species of dipterocarps found in Thailand are not included on that tree. In an effort to complete the tree, the phylogenetic relationships among 23 species belonging to 7 genera of dipterocarps found in Thailand were studies using nucleotide sequences of two intergenic spacer region between trnL-trnF and atpB-rbcL in chloroplast DNA. With Neobalanocarpus heimii as an out group, the molecular tree was consistent with the morphological classification and it was clearly resolved two major groups; Vavate-dipterocarpi and Imbricate-Shorea. The analysis showed that each member genera have similar characteristics and base chromosome number of 11 and 7, respectively. The first group consisted of genus Diptercarpus, Anisoptera, Cotylelobium and Vatica. Shorea and Hopea were in the second group.
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