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Articles by Taghi Naserpour Farivar
Total Records ( 2 ) for Taghi Naserpour Farivar
  Taghi Naserpour Farivar , Mohammad Naderi , Amir Hosein Mohagheghi Fard , Hamid Oskoui Oweisi and Batoul Sharifi Moud
  In the present research, we decided to study the drug resistance rate of isolated strains to the first line of anti mycobacterium drugs: isoniazid, rifampine, ethambutol and streptomycin. All tuberculosis patient who referred to Bou-Ali hospital (the only center for training and treatment of Infectious disease in Sistan and Baluchestan province) during March 2001-July 2003 whose sputum sample smear byZNCF staining were found positive, were included in our study. After primary culture and subsequent passages, the strains of Mycobacterium tuberculosis were identified and isolated according to the growth rate, pigmentation and biochemical tests. Subsequently, drug sensitivity test of isolated was carried out by proportional standard method. In this study on 84 isolated strains of Mycobacterium tuberculosis from patients who referred to Zahedan Bou Ali hospital during March 2001-July 2003 demonstrated drug resistance to Rrifampin, Isoniazid, Streptomycin and Ethambutol was 55.9, 39.2, 33.3 and 27.3%, respectively. Present findings indicated that the prevalence of drug resistance against first line of anti TB drugs will gradually became a serious problem and targeted programs for reduction of these resistance rates is essential.
  Taghi Naserpour Farivar , Amir Hossein Mohagheghi Fard , Shahram Shahraki Zahedani , Mohammad Naderi and Batul Sharifi Moud
  This study was conducted to evaluate the anti Mycobacterium tuberculosis effects of Ocimum sanctum directly and in macrophage culture which were infected with Mycobacterium tuberculosis before treatment with different concentrations of this plant. Suspensions of bacteria were prepared in 7H9 broth and after Macrophage culture, cell suspensions of the M.tuberculosis were added to the attached macrophages. Adherent monolayers was disrupted and bacterial suspensions were serially diluted and plated onto Middle brook 7H10 agar plates. Colonies were counted under a dissecting microscope and reported as CFU. For each culture dilution, six replicate samples were plated and the mean number of colonies was calculated and then Intracellular and extra cellular killing of Ocimum sanctum extracts were measured by colony counting. Present findings showed that in a defined laboratory and macrophage culture, Ocimum sanctum has a potent anti-Mycobacterium tuberculosis effects both directly and in infected macrophage culture. In this study we confirmed anti-tuberculosis effects of different concentration of O. sanctum extracts in vitro and in Macrophage culture but key components of anti tuberculosis action of these extracts and their mechanisms of actions must be discovered in future researches.
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