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Articles by T.T. Liu
Total Records ( 3 ) for T.T. Liu
  G.T. Cao , Y.P. Xiao , C.M. Yang , A.G. Chen , T.T. Liu , L. Zhou , L. Zhang and P.R. Ferket
  A total of 600, 1 day old male Lingnan Yellow broiler chickens were used to investigate the effects of Clostridium butyricum (C. butyricum) on growth performance, nitrogen metabolism, intestinal morphology and cecal microflora in broiler chickens. The birds were randomly assigned into 5 treatments (6 replicate pens per treatment with 20 birds per pen) and fed the same antibiotic-free basal diets during a 42 days feeding experiment. The treatments were as follows: no addition (Control), 2.5x107 cfu C. butyricum kg-1 of diet (CB1), 5x107 cfu C. butyricum kg-1 of diet (CB2), 1x108 cfu C. butyricum kg-1 of diet (CB3) and 10 mg colistine sulfate kg-1 of diet (Antibiotic). Compared with the control birds, birds fed either CB1 or CB2 or antibiotic diet had greater (p<0.05) Body Weight (BW) on day 21 and 42 and higher (p<0.05) Average Daily Gain (ADG) from day 1-42. Birds fed C. butyricum or antibiotic diet had lower (p<0.05) Feed-to-Gain ratio (F:G) than the control birds from day 1-42. Dietary C. butyricum decreased (p<0.05) the concentration of serum Uric Acid (UA) compared with the control diet on day 21. Supplementation with CB2 or CB3 decreased (p<0.05) serum ammonia concentration compared with the control diet on day 21 and 42. Birds fed C. butyricum diet had higher (p<0.05) ileal villus height than the control birds on day 21 and 42. Birds fed CB2 or CB3 diet had lower (p<0.05) ileal crypt depth than the control birds on day 21 and 42. Supplementation with CB1 or CB3 decreased (p<0.05) the population of cecal Escherichia coli (E. coli) compared with the control on day 21. Birds fed C. butyricum diet had higher (p<0.05) population of cecal Bifidobacterium on day 21 and birds fed CB2 diet had higher (p<0.05) cecal Bifidobacterium on day 42 compared with the control birds. Supplementation with CB1 or CB2 increased (p<0.05) the number of cecal Lactobacillus on day 21 and supplementation with CB2 increased (p<0.05) Lactobacillus on day 42 compared with the control or antibiotic groups. The results indicate that supplementation with C. butyricum promotes growth performance, modulates nitrogen metabolism improves intestinal morphology and balances cecal microflora in broiler chickens.
  Chen Min , Wang Lei , T.T. Liu , Song Hui , H.Z. Liu and K.M. Peng
  The present study was performed to investigate the morphology and topographic distribution, developmental changes of somatostatin-producing cells in the small intestinal of African ostrich chicks at different ages: 1 day (birth), 45 days, 90 (days) and 334 (control). The results were as follows: Somatostatin (SOM) cells were present in the mucosal layer of whole small intestinal of the African ostrich on all checked days. SOM cells had round and spherical shapes (closed-type cells) or spindle and pyriform shapes (open-type cells). SOM cells were localized preferentially in the duodenum of 90 days old ostrich. The quantity of SOM-IP cells increased gradually after birth until 90 days and peaked on 90 days in the duodenum, meanwhile it decreased gradually after birth until 90 days and footed on 90 days in the jejunal and ileal regions. These results indicated that SOM might be involved in functional and developmental regulation of small intestinal of African ostrich chicks.
  L. Wang , K.M. Peng , M. Chen , X.T. Zheng , T.T. Liu , C.Y. Jin and W.W. Cao
  The research was carried out on 1-90 days old African ostrich chicks to study the morphological characteristics of tibia development. The length, weight and Bone Mineral Density (BMD) and the developmental characteristics of microstructure and ultrastructure were measured and analyzed in the research. Results showed: ostrich chicks <90 days grew fast and the length, weight and BMD of the tibia all increased significantly and continuously. In the tibia of 1 day old bird, transitional trabeculae constituted the primary cancellous bone which constituted the early formation of backbone. Lots of osteoblasts cling to the surface of cartilage formed thin primary cancellous bone; transitional bone trabeculae were very thin. In the tibia of 45 days old bird, resorption was very active, transitional bone trabecula was absorbed by osteoclasts and then rebuilded. The calcification process of cartilage matrix was active. In the tibia of 90 days old bird, rudiment of new born compact bone and osteons had already existed but the mature bone was still not formed. The study provides foundation for the further study on the influencing factor about tibia development and its molecular mechanism. Simultaneously, it gives the theoretical basis for breeding disease prevention and treatment.
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