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Articles by T. Suzuki
Total Records ( 7 ) for T. Suzuki
  S. Alam , T. Suzuki and T. Katayama
  Syringylglycerol-8-O-4'-(sinapyl alcohol) ethers (SGSEs) are lignin substructure dimeric compounds or 8-O-4' neolignans. To investigate biosynthesis of the 8-O-4' neolignans, incubation of sinapyl alcohol (SA) with enzyme preparations of Eucommia ulmoides was performed and formation of optically active SGSEs was found. To clarify the stereochemical mechanism of the SGSE formation, absolute configuration of four stereoisomers, (+)-erythro, (–)-erythro, (+)-threo and (–)-threo isomers, of SGSEs that contain a chiral secondary benzyl alcohol were determined as (7R, 8S), (7S, 8R), (7S, 8S) and (7R, 8R), respectively, by Mosher’s method [1H NMR analysis of tri-(R)-(+)-α-methoxy-α-trifluoromethylphenylacetate (MTPA) of SGSEs] with our related empirical rules. Four stereoisomers of SGSEs were obtained by dehydrogenations of SA with FeCl3 followed by reversed phase HPLC and chiral HPLC.
  S Rathore , O Katoh , H Matsuo , M Terashima , N Tanaka , Y Kinoshita , M Kimura , E Tsuchikane , K Nasu , M Ehara , K Asakura , Y Asakura and T. Suzuki

Background— Retrograde approach through collaterals has been introduced for percutaneous recanalization of chronic total occlusion (CTO) of the coronary arteries. We investigated the safety and efficacy of retrograde approaches used for percutaneous recanalization of CTO in a consecutive series of patients.

Methods and Results— We studied 157 consecutive patients who underwent retrograde CTO recanalization between 2003 and 2008 at a single center. A total of 118 (75.2%) of these patients have had previously failed antegrade attempts. Septal, epicardial, and saphenous vein graft collaterals were used in 67.5%, 24.8%, and 7.6% of cases, respectively. Collateral channel was crossed by guide wire successfully in 115 (73.2%) cases, and the procedure was successful by retrograde approach in 103 (65.6%) cases. Collateral channels (CCs) were graded as follows: CC0, no continuous connection; CC1, continuous thread-like connection; and CC2, continuous, small sidebranch-like connection. CC1, collateral tortuosity <90°, and angle with recipient vessel <90° (P<0.0001) were significant predictors of success. Epicardial channel use (P=0.01), CC0, corkscrew channel (P<0.0001), angle with recipient vessel >90° (P=0.0007), and nonvisibility of connection with recipient vessel were found to be significant predictors of procedural failure. The CC dissection was observed in 6 patients, with 1 needing coil embolization and others who were managed conservatively. The major adverse cardiac events were low, with 1 coronary artery bypass graft, 1 Q-wave myocardial infarction, 5 non–Q-wave myocardial infarctions, and no deaths in this group of patients.

Conclusions— The retrograde approach in CTO percutaneous coronary intervention is effective in recanalizing CTO. The success rate by retrograde approach was 65.6%, and final success was 85% in this group with acceptable overall adverse events. We have identified predictors of failure related to collateral morphology.

  Y Haga , K Totani , Y Ito and T. Suzuki

During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER). These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing. However, the molecular nature of the transporter remains to be determined. Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives. In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody). The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity. The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent. The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes. This new assay method will be a useful tool for identifying the transporter protein on the ER membrane.

  K. Kawasumi , T. Suzuki , M. Fujiwara , N. Mori , I. Yamamoto and T. Arai
  Researchers attempted to establish temporary criteria to detect hyperlipidemia at early stage in dogs. To verify the usefulness of the criteria, researchers investigated plasma Glucose (GLU) Triglyceride (TG), Total Cholesterol (TC) and Non-Esterified Fatty Acid (NEFA), Alanine Aminotransferase (ALT) and insulin levels as diagnostic markers in 38 clinically healthy dogs. Hyperlipidemia dogs were detected based on the any two of the following three factors, namely elevated the TG, TC and NEFA levels. In addition, measurement of raised insulin levels, the dogs were diagnosed as hyperlipidemia with insulin resistance. Based on these criteria, nine (23.7%) of 38 dogs were diagnosed as hyperlipidemia. In these dogs, plasma TG, NEFA and insulin levels were significantly higher than those in the control dogs without hyperlipidemia (n = 29).
  S. Falah , T. Suzuki and T. Katayama
  Chemical constituents of the bark of Swietenia macrophylla King (Meliaceae) was investigated not only to develop further bark utilization but also to understand the biochemical function of the bark in the forest environment. A new phenylpropanoid-substituted catechin, namely, swietemacrophyllanin [(2R*,3S*,7"R*)-catechin-8,7"-7,2"-epoxy-(methyl 4",5"-dihydroxyphenylpropanoate)] (1) was isolated from the bark of S. macrophylla together with two known compounds, catechin (2) and epicatechin (3). The structure of 1 was elucidated by spectroscopic data and by comparison of the NMR data with those of catiguanins A and B, phenylpropanoid-substituted epicatechins. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity of the isolated compounds indicated that all of the three compounds have strong activity compared with trolox as a reference. Swietemacrophyllanin (1) had the strongest activity with a 50% inhibitory concentration (IC50) value of 56 μg mL-1.
  Rabbani M.A. , A. Iwabuchi , Y. Murakami , T. Suzuki and K. Takayanagi
  In order to assess the potential of oilseed mustard in the breeding programs, 52 accessions were grown in the field and characterized for different morphological characteristics. The germplasm was collected from Punjab and N. W. F. Province of Pakistan in 1994 and evaluated for morpho-physiological traits under field conditions at Tsukuba. Japan during 1995 and 1996. Thirty-five phenotypic traits were recorded for all accessions from seedling emergence till crop harvest. A considerable level of genetic variation was observed among all tested accessions for various traits. However, most of the germplasm collections from Pakistan shared similar morphology with one another when compared with the leafy vegetable cultivars from Japan. Seedling characteristics showed less variation, while the largest variation was found for flowering and maturity stage characters. Generally, low correlation was observed among different traits, however, some of the related characters were significantly correlated with each other. The results indicated that oilseed mustard in Pakistan has narrow genetic base and experiencing high level of genetic erosion perhaps due to selection for similar traits, replacement by new uniform varieties and socio-economic changes in agriculture. Therefore, future germplasm collections should be focused to the unexplored, unique peripheral and more isolated areas of the country. Introduction of germplasm from abroad and hybridization in the available material will also be helpful to broaden the gene-pool of oilseed mustard in Pakistan.
  T Takahashi , H Satoh , M Takaguchi , S Takafuji , H Yokoyama , S Fujii and T. Suzuki

Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression system from the HA gene of A/duck/HK/313/4/78 (H5N3), bound to sulphatide in a dose-dependent manner and that the binding was inhibited by a specific antibody. Our results indicate that the recombinant HA is useful for elucidation of the binding domain of HA with sulphatide and for the development of new anti-IAV agents.

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