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Articles by T. Sutabutra
Total Records ( 2 ) for T. Sutabutra
  W. Pitakdantham , T. Sutabutra , P. Chiemsombat and C. Pitaksutheepong
  Dendrobium Sonia ‘Earsakul’ and Dendrobium Red Bull are widely cultivated for commercial production as cut flower orchid in Thailand. Their purple flower colors are controlled by a series of enzyme which contribute to production of anthocyanin pigments. This study was aimed to isolate dihydroflavonol 4-reductase gene, one of the key enzyme in anthocyanin synthetic pathway from two Dendrobium orchids and to study gene expression pattern during developmental stages of flower. A full-length cDNA clone of DFR gene was obtained from both Dendrobium cultivars. The cDNA clones contained 1,059 bp and encoded 352 amino acids. Multiple alignment of amino acid sequences showed that the dihydroflavonol 4-reductase of Dendrobium Sonia ‘Earsakul’ and Dendrobium Red Bull exhibited high homology to those of Dendrobium hybrid ‘Uniwai Prince’ and Dendrobium hybrid ‘Greeting Fragrance’(99% identity). Phylogenetic tree revealed that the dihydroflavonol 4-reductase of Dendrobium orchids were closer relatedness than those of other orchid plants like Bromheadia and Oncidium. Relative quantification analysis of dihydroflavonol 4-reductase gene expression in floral tissues at five developmental stages of Dendrobium Sonia ‘Earsakul’ and Dendrobium Red Bull showed that expression of the dihydroflavonol 4-reductase gene was largely detected in young flower buds at the early stage of pigmentation. In open and fully colored flowers, transcription of the gene was decreased to an undetectable level.
  W. Pitakdantham , T. Sutabutra , P. Chiemsombat and C. Pitaksutheepong
  To isolate and characterize chalcone synthase gene in anthocyanin biosynthetic pathway during flower development of Dendrobium Sonia Earsakul. The gene was isolated from floral tissues of the orchid by reverse transcriptase polymerase chain reaction. Characterization of the gene considered to its relatedness to chalcone synthase gene in other orchid plants elucidated by construction of a neighbor-joining phylogenetic tree. Gene expression pattern related to flower development and pigmentation was investigated by relative quantification real time polymerase chain reaction. A complete coding sequence was obtained and sequence analysis revealed that the gene of Dendrobium Sonia Earsakul consisted of 1,188 bp. Blast analysis and multiple alignments showed that the chalcone synthase gene of Dendrobium Sonia Earsakul shares high homology to chalcone synthase gene of Dendrobium genus particularly Dendrobium hybrid Uniwai prince. Phylogenetic tree revealed that chalcone synthase of Dendrobium genus are highly conserved. The chalcone synthase gene of Dendrobium Sonia Earsakul was highly expressed in young flower bud with no pigmentation and the expression was sharply decreased when young flower bud started accumulation of pigments. Expression of chalcone synthase gene was then maintained at the same level until young bud developed into fully opened flowers.
 
 
 
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