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Articles by T. Okamoto
Total Records ( 2 ) for T. Okamoto
  N. Nurmeiliasari , R. Priyanto , D.A. Astuti , Salundik , J. Takahashi , M. Okamoto , T. Okamoto and A. Jayanegara
  Background and Objective: The effects of rumen mechanical stimulating (RMS) brushes on the health of Brahman crossbred steers were determined by measuring blood parameters and examining the rumen wall. Materials and Methods: This study used twenty Brahman crossbred steers divided into control and RMS groups. The steers were given low-forage diets (95.5% concentrate and 4.5% maize stover) based on 3% dry matter by body weight. The installation of RMS was conducted by a professional. Blood samples were collected at the end of the experiment from the coccygeal vein for blood profile and immunoglobulin G (IgG) measurements. The steers were slaughtered at the 93rd day of the experiment, samples of the rumen wall were collected and rumen histological observations were made. Results: The results showed that the RMS brush administration did not affect the hematocrit, hemoglobin (Hb), number of red blood cells (RBC), number of white blood cells (WBC) or IgG (p>0.05). However, there was a significant increase in neutrophils (p<0.05). The steers with RMS had a higher ratio of neutrophils to lymphocytes (p<0.05). Even though the treatment affected the neutrophil count and the ratio of neutrophils to lymphocytes, these values were in a normal range. The results of the rumen gross anatomy and histology observations showed that there was no tissue damage of the rumen, thus, it is in a normal state. Conclusion: The data indicate that the RMS brush utilization in steers does not result in adverse effects on the health of steers as measured by blood profiles, IgG concentrations and rumen histology.
  F. Chen , J. Zhang , A. Aoyama , T. Okamoto , T. Fujinaga and T. Bando

Objective: A method to compensate for the donor shortage may be the utilization of donation after cardiac death. The control of lung injury against warm ischemia is crucial in manipulating donors after cardiac death. Nebulization is a simple and feasible drug delivery route after cardiac death. Herein we have examined the potential effect of nebulized milrinone, a phosphodiesterase III inhibitor, on pulmonary warm ischemia.

Materials and Methods: Deeply anesthetized rats were euthanized by exsanguination. Lungs were exposed to warm ischemia with ventilation up to 2 hours. Milrinone was nebulized for 10 minutes at the beginning of warm ischemia (n = 5). In the control group (n = 5), normal saline was nebulized for the same time. At given intervals, the lungs were partially resected to measure adenine nucleotide and cyclic adenosine monophosphate levels.

Results: In both groups, lung tissue cyclic adenosine monophosphate levels decreased significantly at 2 hours after warm ischemia; however, there was no significant difference between the groups. Lung tissue adenosine triphosphate levels significantly decreased at 2 hours after ischemia in the control group, while they did not drop up to 2 hours in the milrinone group. Further, lung tissue adenosine triphosphate levels at 2 hours after ischemia were higher in the milrinone group than the control group.

Conclusions: Our results confirmed that milrinone nebulization during warm ischemia maintained lung tissue adenosine triphosphate levels. Therefore, milrinone nebulization may have potential for lung protection against warm ischemia.
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