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Articles by T. Ohe
Total Records ( 1 ) for T. Ohe
  M. Mohseni , T. Ohe , M.R. Fazeli , S.N. Ostad , M. Hamedi , H. Jamalifar and E. Azizi
  In this study, mutagenic potential of red florets of Iranian Safflowers IL 111 was evaluated by Ames test using Salmonella typhimurium TA98 and TA100 strains. Florets collected and dried in two conditions, dried on shade and dried on the bolls by sun. Extractions have been done with 70% hydro-ethanolic solution and also boiling water. Solvents were then evaporated from extracts by freeze drier. Different dilutions of extracts were prepared in either distilled water or DMSO to be evaluated in the presence and absence of S9 bioactivation. Test and control plates were prepared according to the plate incorporation method and incubated for 48 h at 37°C. Then the number of reverted colonies was counted to determine the Mutation Ratio (MR) for each test concentration. The MR for test samples was far less than positive controls to be considered mutagen. There was also no significant difference in MR of extracts before and after S9 bioactivation at studied concentrations. Therefore, it can be concluded that Safflower extracts have no mutagenic activities under different preparatory and assay conditions using Ames mutagenicity assay.
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