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Articles by T. Hartatik
Total Records ( 3 ) for T. Hartatik
  S. Johari , N. Setiati , J.H.P. Sidadolog , T. Hartatik and T. Yuwanta
  The aim of this study was to determine the gene effect of Growth Hormone (GH) on divergent selection of Japanese quail. Quails were grouped into high weight (Q-H), low weight (Q-L) and random weight (Q-R) females as a treatment for divergent selection. Parameter phenotype observed in each generation is the weight at four weeks of age and egg production at ten weeks of age for five generations. The results showed that the dominance level on body weight of Q-L was incomplete dominance, Q-R was over dominance and Q-H was lack of dominance. While the dominance level on egg production of Q-L and Q-H were over dominance and Q-R was lack of dominance. The gene effect of GH on body weight of Q-H is 1.53 times greater than the Q-L and 12.37 times greater when compared with Q-R. Whereas the gene effect of GH on egg production of Q-H is 1.53 times greater than the Q-L but only 4 times greater when compared with Q-R. Should be developed that to increase the low-weight (Q-L) is in the BB genotype and the high weight (Q-H) is in the AA genotype groups. Otherwise, to increase the low-weight (Q-L) and high-egg production are in the AA and BB genotypes and the high weight (Q-H) and low-egg production are in the AA and AB genotypes groups.
  M.T. Beigi Nassiri , Z. Biranvand , T. Hartatik , J. Fayazil and S. Tavakoli
  The part of the bovine genome which shows a superior action and explains the major part of variation of the economical production traits were known as QTL. PIT1 gene which is also termed hormone factor-1 is a pituitary-specific transcription factor which has responsible for pituitary development and hormone expression in mammals. The main function of PIT1 is for binding and trans-activity promoters of both Growth Hormone (GH) and Prolactin (PRL) gene. It was subjected to different molecular studies as a key role for understanding genetic variation in dairy cattle. This study was aimed to detect the polymorphism in a part of PIT1 gene, exon 6 in Najdi cattle. DNA was extracted from blood sample collected from 84 Najdi Cattle and submitted for Polymerase Chain Reaction (PCR) followed by digestion with HinfI restriction enzyme. Base on PCR-RFLP method, it found that the frequency of the A and B alleles of this gene for Najdi Cattle were 18.45 and 81.55%, respectively. The genotypic frequencies of AA, AB and BB were 3.57, 29.76 and 66.66%, respectively. χ2-test indicated that these two populations were at Hardy-Weinberg equilibrium. Thus, the selection base on these loci in the population is possible to be done.
  M. T. Beigi Nassiri , Z. Beyranvand , T. Hartatik , J. Fayazi and S. Tavakoli
  The part of the bovine genome which shows a superior action and explains the major part of variation of the economical production traits were known as QTL. PIT1 gene which is also termed hormone factor-1 is a pituitary-specific transcription factor which has responsible for pituitary development and hormone expression in mammals. The main function of PIT1 is for binding and trans-activity promoters of both Growth Hormone (GH) and Prolactin (PRL) gene. It was subjected to different molecular studies as a key role for understanding genetic variation in dairy cattle. This study was aimed to detect the polymorphism in a part of PIT1 gene, exon 6, in Najdi cattle. DNA was extracted from blood sample collected from 84 Najdi cattle and submitted for Polymerase Chain Reaction (PCR) followed by digestion with HinfI restriction enzyme. Base on PCR-RFLP method, the researchers found that the frequency of the A and B alleles of this gene for Najdi cattle were 18.45 and 81.55%, respectively. The genotypic frequencies of AA, AB and BB were 3.57, 29.76 and 66.66%, respectively.
 
 
 
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