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Articles by T. Ahamed
Total Records ( 3 ) for T. Ahamed
  M.M. Rahman , M.N. Amin , T. Ahamed , M. R. Ali and A. Habib
  Somatic embryogenesis and subsequent plantlets regeneration were achieved in callus cultures established from the leaf base explants of Kaempferia galanga L. Callus induction and somatic embryogenesis at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed on MS medium supplemented with 1.5 mg L-1 2,4-D+1.0 mg L-1 BA. After transfer on MS medium supplemented with 2.0 mg L-1 BA+0.1 mg L-1 NAA, this callus produced small globular embryos first that later developed into plantlets by further subculturing on the same medium. Plantlets were acclimatized and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 85%.
  T. Ahamed , K.M. Hossain , M.M. Billah , K.M.D. Islam , M.M. Ahasan and M.E. Islam
  Newcastle disease virus (NDV) is the infectious agent of Newcastle disease in poultry. This virus can grow within different animal cells including primary cell culture and established cell line. In order to adapt NDV on African green monkey kidney (Vero) cell line, five consecutive passages were done. Eagle`s minimum essential medium (EMEM) with supplements was used for both culturing Vero cells and maintaining NDV on Vero cells. During first and second passage, wild NDV didn`t produce any clear evidence of cytopathic effect (CPE), but in third passage changes in the characteristics of cell monolayer were observed. During fourth and fifth passages, clear and consistent CPE were observed within 50 to 60 hours of infection. CPE was characterized by formation of syncytium, giant cell, dendritic-shaped cell and finally plaque. The titer of passage 5 (P5) virus was 10-3.9TCID50, whose purity was tested by serum neutralization test (SNT) and the result was 1.6 x 104 units/ml.
  M.M. Rahman , M.N. Amin , T. Ahamed , S. Ahmad , A. Habib , R. Ahmed , M.B. Ahmed and M.R. Ali
  An efficient protocol has been established for rapid production of plantlets using rhizome tip and lateral bud explants of the field grown plant. The explants were cultured on MS medium with auxins (NAA, IBA and IAA) and cytokinins (BA and Kn). Cent percent of the explants produced two or three shoot buds in each culture when they were cultured on MS medium containing 1.0 mg L -1 BA+0.1 mg L -1 NAA within three weeks of culture. The number of shoots per culture increased gradually when the primary cultures were subcultured in two weeks intervals. Highest number of 20.50±1.80 shoots proliferated in each culture when the explants of initially sprouted shoots were subcultured at three times on the same medium. Microshoots were isolated from the in vitro proliferated cluster of shoots produced roots in 100% cases on modified (MMS2) medium supplemented with 0.2 mg L -1 of IBA. Maximum number of 12.4±1.23 roots per microshoot were recorded on the medium containing 0.2 mg L -1 IBA. The regenerated plantlets were acclimatized and established on the soil with eighty five percent success.
 
 
 
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