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Articles by Syed Enamul Hossain
Total Records ( 1 ) for Syed Enamul Hossain
  Syed Rashel Kabir , Syed Enamul Hossain and Nurul Absar
  Two lectins from the rice bran of Amon variety were purified by successive chromatography of 100% ammonium sulfate saturated crude extract on Sephadex G-50 followed by ion-exchange chromatography on DEAE-cellulose. Both the lectins are found to be homogenous as judged by polyacrylamide gel electrophoresis. The molecular weight of the lectins, RBL-1 and RBL-2 as determined by gel filtration and SDS-PAGE were estimated to be as 22,000 and 36,000 respectively. The lectins are dimer and their subunits are held together by disulfide bonds. The lectins agglutinated rat red blood cells and the agglutination was inhibited specifically by D-glucose, D (+) glucosamine-HCl and N-acetyl-D-glucosamine. The lectins are glycoprotein in nature with neutral sugar content of 4 - 5.4%. The lectins RBL-2 are more cytotoxic than RBL-1 as observed by brine shrimp nauplii mortality rate bioassay.
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