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Articles by Swati Sucharita Dash
Total Records ( 2 ) for Swati Sucharita Dash
  Swati Sucharita Dash and Sathyanarayana N. Gummadi
  Pseudomonas sp. NCIM 5235 capable of degrading high concentrations of caffeine has been previously isolated from the soil of coffee plantation area. The isolate was capable of degrading 6.4 g L-1 initial concentration of caffeine at a rate of 0.1 g L-1 h-1. In this study, the physical parameters viz., pH, temperature and shaking speed have been optimized using central composite design. The optimum values of pH, temperature and shaking speed were found to be 7.8, 28°C and 190 rpm, respectively. Under optimized condition of pH, temperature and shaking speed, the rate of degradation of caffeine has been enhanced from 0.18 to 0.29 g L-1 h-1 which is 1.6 fold higher than the normal rate. This is the first report on degradation of high concentration of caffeine at higher rates. Under optimal conditions, the strain has also been found to degrade caffeine at 15 g L-1 initial concentration efficiently within 48 h. This makes Pseudomonas sp. NCIM 5235 an attractive candidate for development of biodecaffeination strategies.
  Swati Sucharita Dash and Sathyanarayana N. Gummadi
  The study was undertaken to investigate the effect of caffeine (1, 3, 7- trimethylxanthine) on growth, morphology and viability of caffeine degrading Pseudomonas sp. and other non caffeine degrading bacterial strains. Growth, morphology and cell viability of the bacterial strains were studied in caffeine medium, minimal medium without caffeine and in minimal medium with caffeine added at log phase of growth. The caffeine degrading Pseudomonas sp. achieved a maximum cell dry weight of 1.1 g L-1 after caffeine addition at log phase of growth without any change in morphology. The growth and viability of E. coli DH5α and other bacterial strains was greatly reduced upon addition of caffeine at log phase of growth. E. coli DH5α strain formed long filamentous cells on caffeine exposure and lysis occurred for other Gram negative bacterial species and Bacillus subtilis. E. coli DH5α transformed with plasmid from caffeine degrading Pseudomonas sp. was found to be tolerant to caffeine. This study shows the susceptibility of non caffeine degrading bacterial strains to caffeine and will be helpful in understanding the basics of the evolution and survival of xenobiotic degrading strains in nature.
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