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Articles by Suzanne M. Wagdy
Total Records ( 3 ) for Suzanne M. Wagdy
  Suzanne M. Wagdy , Sahar H. Mohamed and Fakhriya S. Taha
  Jojoba defatted meal contains toxic compounds mainly simmondsin and simmondsin 2-ferulate. The aim of the present investigation was to study the solubility pattern of simmondsin, simmondsin 2- ferulate, protein, non-protein nitrogen and phenolic compounds present in Jojoba meal at pH ranging from 1-12. Both the supernatant and precipitate resulting after extraction at a certain pH were analyzed for the above mentioned components. The simmondsins were identified and quantified by thin layer chromatography. The antimicrobial activities of the 12 pH extracts were evaluated. Results revealed that the precipitate containing lowest simmondsin 0.55, 0.55 and 0.74 g/100 g meal was achieved at pH 1, 2 and 12, respectively. Meanwhile simmondsin 2-ferulate amounted to 0. 67, 0.72 and 0.72 g/100 g meal, at pH 9, 1 and 2, respectively. Isoelectric point of jojoba meal protein showed to be between pH 3-4 with least solubilized protein 14.26-14.08% and highest precipitated protein in the residue 17.74-17.92% protein. Non-protein nitrogen ranged between 2-3.5% in supernatant and between 7-8.6% in the precipitate. Phenolic compounds extracted in the supernatant increase with increasing pH except at pH 4 and 8 where they exhibited some decrease. Normally, the phenolic compounds in the residue followed an opposite trend. Extract at pH 1 inhibited the growth of the five examined bacteria strains. Extracts of jojoba meal resulting from pH 2, 5, 6 and 8 showed inhibition of only one of the five bacterial strains. In conclusion, simmondsins can be effectively removed from the jojoba meal at pH 1, 2 and 12. Jojoba extract at pH 1 exhibited good antibacterial activity.
  Suzanne M. Wagdy , F.S. Taha and Salma S. Omar
  Objective: The objectives of this study were to reduce the toxic compounds and polyphenolic compounds in jojoba meal by (1) One solvent with one concentration (100%) and different techniques, (2) Different solvents and different concentrations with the same technique and (3) Illustrate its effection on native and protein bands. Methodology: The jojoba press cake was defatted in a soxhelt apparatus, the dried defatted meal was extracted with different solvents and different techniques to reduce both phenolic and simmondsins content. Results: The results showed that maximum simmondsins and phenolic extractes were 14% and 18.5 mg g–1 meal, respectively. The best achieved condition to extract simmondsins was soxhlet extraction for 30 h whereas, in case of phenolic compounds, shaking for 15 min, then soaking for 72 h was the best condition. The examination of the dried residue by electrophoresis (native and SDS-PAGE) analysis showed that five major polypeptide bands (10, 15, 20, 38 and 48 kDa) were predominant with similar ratios in almost all used techniques, no reduction in protein bands with 100% solvent, whereas variations in solvent concentrations caused reduction in protein bands and the protein mobility increased with increasing the effect of microwave time. Conclusion: The protein analysis revealed that there is no relation between the simmondsins content and the protein quality.
  Suzanne M. Wagdy , F.S. Taha and Salma S. Omar
  Objective: The objectives of this study were to investigate (1) The possibility of turning waste products into valuable nutraceuticals, (2) Also substituting synthetic pharmaceuticals with neutraceuticals of advisable plant origin and (3) Adding new value with low cost to the main agro-industrial wastes. Methodology: The dried ground jojoba and jatropha hulls were extracted with different solvents at different concentrations. The dried pulverized hulls were first extracted in an ultrasonic water bath then soaked overnight, the extracts were tested for their content of phenolic compounds, flavonoid and triterpene saponins and the extracts were examined for antioxidant activity. For each extract three series of antioxidant activity methods were applied; 2, 2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydrogen peroxide (H2O2) scavenging activity and total reducing-capability. Results: The water and ethanol (50-70%) concentrations is the best solvents to extracts phytochemical compounds with strong antioxidant activity. Conclusion: A simple easy method to extract phenolic, flavonoid and saponin compounds from jojoba and jatrova hulls has been developed. The method uses less solvent and requires less energy. The process used results in a bioactive extract to be used in the pharmaceutical industry and leaves the remaining material after extraction suitable for other industries.
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