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Articles by Sunarti
Total Records ( 9 ) for Sunarti
  Prasetyastuti , Sunarti , Ahmad Hamim Sadewa , Sri Mursiti and Mustofa
  Background and Objective: Type 2 diabetes is characterized by hyperglycemia leads to increased production of reactive oxygen species. There is increasing evidence that active compounds of medicinal plants may be used to reduce oxidative stress. The aims of this study were to investigate anti-diabetic effects of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one derived from Swietenia macrophylla King seed on oxidized LDL, homeostatic model assessment beta-cell function (HOMA-β) and glucagon like peptide 1 (GLP-1) gene expression in diabetic rats. Materials and Methods: A total of 30 rats were used. They were divided into 6 groups as follows: (A) Normal rats, (B) Diabetic rats, (C) Diabetic rats with metformin, (D), (E) and (F) Diabetic rats with 7-Hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chromen-4-one Swietenia macrophylla King seed dose 10, 30 or 90 mg/200 g b.wt., respectively. Oxidized LDL and HOMA-β value were analyzed twice, before and after treatment. The GLP-1 gene expression in intestine was analyzed at the end of study. Data were analyzed by paired t-test and one-way ANOVA followed by Games-Howell test. Results: Administration of three different doses of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds in diabetic rats significantly reduced oxidized LDL and increased HOMA-β value (p<0.001) and also GLP-1 gene expression (p<0.05). Conclusion: These findings demonstrate that administration of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds, improved beta cell function through reducing oxidized LDL and increased GLP-1 expression.
  Prasetyastuti , Adhi Tanjung Laksono , Ricky Taufiqurrohman , Tantra Dewi Rahardyanti , Gandhi Anandika Febryanto , Yan Mahmud Fau , Sri Herwiyanti , Ngadikun and Sunarti
  Background and Objective: Carbon tetrachloride (CCl4), a carcinogenic compound commonly used in household cleaning agents and spot removers can cause free radical-mediated liver damage. Recent research shows polyphenols in pomegranate (Punica granatum L.), rich in exogenous antioxidants, may protect the liver from hepatotoxic damage. This study aimed to investigate the effect of pomegranate juice on CCl4-induced liver damage in a rat model. Materials and Methods: A total of 25 rats, aged 8 weeks and weighing an average of 200 g were used. They were divided into 5 groups as follows: (1) Normal, (2) Hepatotoxic rats and (3), Hepatotoxic rats with pomegranate juice 0.4 mL/200 g body weight (BW) (4) Hepatotoxic rats with pomegranate juice 0.8 mL/200 g BW and (5) Hepatotoxic rats with pomegranate juice 1.2 mL/200 g BW, respectively. Hepatotoxicity was done by intraperitoneal injection of 0.5 mL–1 CCl4 kg–1 BW for 5 days. Before and after 2 weeks administration of treatment, blood samples were collected for assessment of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (γ-GT) and malondialdehyde (MDA). At the end of the study, the animals were euthanized and the livers were used for histopathological assessment. Data were analyzed by paired t-test and one-way ANOVA followed by the Games-Howell test. Results: Supplementation with three different doses of pomegranate juices in CCl4-induced rats significantly reduced the serum levels of ALT, AST, ALP, γ-GT and MDA (p<0.05) and pomegranate juice as much as 0.8 and 1.2 mL/200 g BW reduced liver damage in CCl4-induced rats. Conclusion: According to findings of this study supplementation with pomegranate juice can improve liver damage induced by CCl4 in rats.
  Nurliyani , Eni Harmayani and Sunarti
  The microbiological quality, fatty acid and amino acid profiles were studied in kefir prepared from combination of goat and soy milk (100:0), (50:50) and (0:100). Total counts, total of lactic acid bacteria (LAB) and total yeast of kefir were counted with Standard Plate Count (SPC). Fatty acids composition was analyzed by gas chromatography (GC), whereas amino acids composition was analyzed by high performance liquid chromatography (HPLC). The results showed that there were no significant difference in total counts, LAB and yeast of all kefir. The acidity of kefir made from soy milk only was lower (p<0.05) than kefir made from goat milk or combination of goat and soy milk, whereas there were no significant difference in pH. Caproic (C6:0), heptadecanoic (C17:0), behenic (C22:0) and pentadecanoic (C15:1) acid of kefir made from 50% goat milk and 50% soy milk mixture (50:50) were lower (p<0.05) than goat milk kefir (100:0), but the oleic acid (C18:1) of kefir made from combination of 50:50 was higher (p<0.05) than 100:0. The amino acids composition of kefir made from goat milk only (100:0) and combination 50:50 was not significantly different. Therefore, this study suggests that soy milk can substitute 50% of goat milk to produce kefir without changing the microbiological quality, acidity, pH value, amino acids profile and even increase the oleic acid.
  Sunarti , Rio Jati Kusuma , Dianandha Septiana Rubi and Hemi Sinorita
  In type 2 diabetes mellitus, waist circumference was associated with increase of plasma free fatty acid and improvement in insulin resistance. Crispy arrowroot flake contain high fiber content and has a low glycemic index value which is expected to lower waist circumference through lipolysis. This study was conducted to determine the effect of the crispy arrowroot flake on waist circumference and plasma free fatty acid in type 2 diabetes mellitus. A quasi-experimental study was conducted on 30 type 2 diabetic patients who regularly visited endocrine clinic of RSUP Dr. Sardjito Yogyakarta. The criteria’s participants are age 35-60 years, fasting blood glucose > 126 mg / dL and they aren’t smoking, pregnant, lactating and hypertension. The 7 grams of flake was given 3 times per day for 8 weeks. Waist circumference and blood free fatty acid were measured before and after 8 weeks intervention. Consuming of the flake can reduce waist circumference and increase free fatty acid. Waist circumference was lower in men (86.35±6.78 vs 85.30±6.38, p = 0.453) than in women (84.74±8.92 vs 83.76±7.81, p = 0.176). Free fatty acid was found increase in men (0.29±0.512 vs 0.633±0.26, p = 0.231), whereas no change was observed in women (0.837±0.26 vs 0.834±0.32, p = 0.962). Consumption of the crispy arrowroot flake can reduce waist circumference that is more amount in men than in women type 2 diabetes. Increased of free fatty acid only found in men.
  Prasetyastuti , Mochammad Willy Pratama Anthony , Nuha Aulia Rahman , Ngadikun and Sunarti
  Diabetes mellitus is a metabolic disorder that is characterized by hyperglycemia and associated with increased oxidative stress. The polyphenol property in pomegranate juice was reported to have significant antioxidant effect. The present study aims to investigate the hypoglycemic and antioxidative effect of pomegranate juice in streptozotocin-induced diabetic rats. Twenty healthy male rattus norvegicus with age of eight weeks and weighing at average of 200 g were randomly assigned into four groups. Diabetic models were induced by using 60 mg/kg body weight (BW) streptozotocin (STZ) intraperitoneally. The rats were allocated into four groups, group treated with 0.1 mg/kg BW glibenclamide, groups supplemented with 1, 2 and 4 mL/200 g BW pomegranate juice, respectively. Evaluation of hypoglycemic and antioxidative effect were performed by measuring blood glucose level, lipid peroxidation, including malondialdehyde (MDA) and advanced glycation end-product (AGE) before and after treatment. Lipid profile was also measured before and after treatment. Treatment with glibenclamide and supplementation with three different doses of pomegranate juice in diabetic rats were significantly reduced blood glucose level. Supplementation with 2 mL/200 g BW pomegranate juice was significantly reduced MDA concentration, whereas the AGE concentration was not significantly decreased by pomegranate juice supplementation. Lipid profile related to total cholesterol and triglyceride levels were not significantly reduced. Furthermore, high density lipoprotein was not increased significantly after each treatment. Pomegranate juice has hypoglycemic and antioxidative effects in streptozotocin-induced diabetic rats.
  Prasetyastuti , Singgih Setiawan , Yuwinda Prima Ardelia , Tsurayya Fatma Hanifah , Yunardi Singgo and Sunarti
  Background: Retinol Binding Protein 4 (RBP4) is adipocytokine that it’s secretion and expression in adipose tissue is highly correlated with glucose uptake and insulin sensitivity. Increasing of RBP4 in type 2-diabetes mellitus correlate with insulin resistence. Swietenia macrophylla king contain alkaloid, saponin and flavonoid. Objective: The aim of this study is to investigate the effect of 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline alkaloid compound of Swietenia macrophylla king seed to lipid profile and liver tissue RBP4 expression in type 2-diabetes rats. Methodology: Twenty five adult rattus norvegicus were used, five in each group. Group I: Diabetic rat (untreated), group II: Diabetic rat receive glibenclamid, group III: Diabetic rat receive 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline dose 10 mg/200 g b.wt., day–1, group IV: Diabetic rat receive 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline dose 20 mg/200 g b.wt., day–1 and group V: Diabetic rat receive 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline dose 40 mg/200 g b.wt., day–1. Blood samples were analysed for blood glucose, cholesterol, low density lipoprotein, triglyceride and high density lipoprotein level before and after 4 weeks treatment. At the end of the experiment sample of liver tissue were removed for immunohistochemical analysis. Results: Blood glucose level decreased significantly (p<0.05) in group II, III, IV and V. Cholesterol level decreased non-significantly (p>0.05), Low Density Lipoprotein (LDL) decreased significantly (p<0.05) at dose 40 mg/200 g b.wt. High Density Lipoprotein (HDL) level increased significantly (p<0.05) at doses 10 and 20 mg/200 g b.wt. Conclusion: However, the expression of Retinol Binding Protein 4 (RBP4) decreased after treatment with 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline. These results demonstrated that 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline increased HDL and decreased LDL and RBP4 expression.
  Dina Tri Marya , Nurliyani , Widodo and Sunarti
  Objective: This study aimed to evaluate the microbiological and chemical characteristics and antioxidant activity of fermented milk produced by different starter combinations. Materials and Methods: Nine combinations of starter produced fermented dairy products using single-starter Lactic Acid Bacteria (LAB), a combination of starter LAB and a combination of starter LAB and yeast. The starter combinations were as follows: Lactobacillus plantarum Dad 13, L. plantarum Dad 13+Lactococcus lactis, L. plantarum Dad 13+Saccharomyces cerevisiae, L. plantarum Dad 13+Kluyveromyces marxianus, L. plantarum Dad 13+L. lactis+S. cerevisiae, L. plantarum Dad 13+L. lactis+K. marxianus, L. lactis, L. lactis+S. cerevisiae and L. lactis+K. marxianus. Total LAB and yeast were analyzed using the Total Plate Count (TPC) method. Chemical characteristics were identified by the values of pH, titratable acidity and ethanol contents. Antioxidant activities were determined by calculating the percentage of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Results: Present study showed that the combination of starters did not differ significantly across total LAB and yeast. However, there were significantly differences in pH, acidity, alcohol, β-carotene and antioxidant activity. The fermented milk obtained from L. plantarum Dad 13+L. lactis+K. marxianus had the lowest pH and the highest acidity and alcohol content. The combination L. plantarum Dad 13+L. lactis+S. cerevisiae produced the maximum β-carotene content and the combination of L. plantarum Dad 13+ L. lactis+S. cerevisiae had the best antioxidant activity. Conclusion: It is concluded that a combination of starter L. plantarum Dad 13, L. lactis and S. cerevisiae can be used to improve the chemical quality and antioxidant activity of fermented milk.
  Sunarti , Sri Lestari Sulistyo Rini , Dianandha Septiana Rubi , Miftakhussolikhah , Dini Ariani and Hemi Sinorita
  Background and Objective: Dietary fibers have been identified to have beneficial effects in improving insulin secretion and insulin resistance. This study aimed to evaluate the effects of fiber-rich snacks on insulin secretion and insulin resistance in patients with type 2 diabetes mellitus (T2DM). Materials and Methods: Twenty patients with T2DM, ages 40-60 years were recruited from the Polyclinic of Endocrine, General Hospital Dr. Sardjito, Yogyakarta, Indonesia. The subjects received daily 32 g snacks made from a mixture of Dioscorea esculenta, arrowroot, cassava and pumpkin, for 4 weeks. Fasting plasma glucose, insulin and C-peptide levels were measured before and after intervention. Paired t-tests were used to evaluate the results. Results: Intervention of fiber-rich snacks for 4 weeks for T2DM patients significantly (p = 0.01) reduced fasting plasma glucose from 9.3±2.7 to 8.3±2.5 mmol L–1 and homeostatic model assessment of insulin resistance (p = 0.04) from 4.1±1.3 to 3.6±1.9 and significantly (p<0.001) increased C-peptide levels from 0.417±0.004 to 0.512±0.008 ng mL–1. Conclusion: In this study, fiber-rich snacks provide significant benefits to increase endogenous insulin secretion and reduce insulin resistance in patients with T2DM.
  Danus Hermawan , Pramudji Hastuti and Sunarti
  Background and Objective: High fat and fructose diet causes hypertriglyceridemia that induce production of proinflammatory interleukin Iβ (IL-Iβ). Expression of IL-Iβ can be suppressed by short chain fatty acids (SCFA).This study aimed to determine the effects of high fiber diet on the level and expression of IL-1β in high fat and fructose diet model of Wistar rats. Materials and Methods: Twenty-five Wistar rats divided into 5 groups: Normal control (N), hypertriglyceridemia control (TC), hypertriglyceridemia with 1.04 g rat–1 day–1 of fiber (T1); hypertriglyceridemia with 2.07 g rat–1 day–1 of fiber (T2) and hypertriglyceridemia with 3.11 g rat–1 day–1 of fiber (T3). Triglyceride levels were measured using colorimetric method, whereas IL-1β levels were measured using ELISA method. All biochemical analysis was performed twice, before and after treatment. IL-1β gene expression in white adipose tissue was measured by q-PCR method at the end of the study. Results: Triglyceride levels in treatment groups were lower compared to hypertriglyceridemia control group. Gene expression and levels of IL-1β at treated groups, receiving high fiber diet, were lower compared to the hypertriglyceridemia control group. Conclusion: High fiber diet could suppress the expression of IL-1β in white adipose tissues and could reduce the level of IL-1β in rats.
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