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Articles by Sumaiya Islam
Total Records ( 2 ) for Sumaiya Islam
  Md. Fakruddin , Sumaiya Islam , Monzur Morshed Ahmed , Abhijit Chowdhury and Md. Mahfujul Hoque
  Lack of reliable and rapid method for detection of pathogens from shrimp in Bangladesh is an obstacle in ensuring the microbiological quality of the shrimp before export. Salmonella sp. and Vibrio parahaemolyticus are two potential pathogen for shrimp export value chain in Bangladesh. The objective of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus from export oriented shrimp samples. The targeted genes were tdh for V. parahaemolyticus and sefA for Salmonella spp. The genomic DNA was extracted and amplified for subsequent profiling. Validity of the multiplex PCR assay was tested by artificially inoculating the shrimp homogenate with viable cells of target pathogens. The genes were successfully amplified individually and simultaneously both from natural and spiked samples. Sensitivity of the assay was determined to be as low as 104 CFU mL-1. Amplification of DNA extracted from other bacterial pathogens viz. Bacillus cereus, Shigella flexneri and Staphylococcus aureus yielded negative results. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of target pathogens in a single reaction tube in mixed bacterial communities that are prevalent in shrimp products.
  Shuva Bhowmik , Sumaiya Islam , Monzur Morshed Ahmed , M. Belal Hossain and Md. Abul Hossain
  Proteases, the group of enzyme with significant commercial value, was isolated from proteolytic bacteria available in gut of tiger shrimp (Penaeus monodon). The isolation of bacteria from the gut of the collected shrimp was performed by serial dilution and plating method. Six bacterial isolates were screened out on the basis of their formation of zone of casein hydrolysis on skim milk agar. Among the six isolates, three isolates (S1, S3 and S5) were found gram positive and the rest three isolates (S2, S4 and S6) were found gram negative. Protease activity of the isolates was determined both qualitatively and quantitatively. In qualitative plate assay, isolate S1 exhibited the largest clear zone (30±1.13 mm) in skim milk agar and isolate S5 exhibited the lowest (18±1.41 mm). Quantitative protease assay was performed by using azo-casein as substrate. Protease activity of isolates S1, S2, S3, S4, S5 and S6 were found 49.75±2.13, 60.50±1.97, 66.25±2.41, 56.25±1.69, 59.25±1.32 and 52.75±2.21 U mL-1, respectively following incubation at 37°C in aerobic condition for 24 h. The effect of pH and NaCl concentration on the growth and protease production of the isolates were also studied by assaying protease activity at different pH range and NaCl concentrations. The isolates exhibited maximum protease production at varying pH and NaCl concentrations. The data showed potentiality of the bacterial isolates to be a useful source of industrial protease. Finally, the isolates were also tested against six standard antibiotics (Tetracycline, Nitrofurantoin, Erythromycin, Penicillin, Ciprofloxacin and Doxycycline) to observe their antibiotic sensitivity and the isolates S5 and S6 were found resistant to all of the six antibiotics and isolates S3 and S4 were found sensitive to all of the antibiotics.
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