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Articles by Sukru KIRKAN
Total Records ( 4 ) for Sukru KIRKAN
  Dilek Keskin , Okan Atay , Sukru Kirkan , Ozdal Gokdal , Serten Tekbiyik and Osman Kaya
  This study is performed to identify existence of Streptococcus agalactiae being mastitis factor in hair goats grown in Cine district of Aydin province in West Anatolia region. Material of the study consists of samples of 232 goat milk. Samples have been received from 116 goats of 9 herds grown in Kavsit, Tatarmemisler, Catak, Ibrahimkavagi towns of Cine district. Samples have been delivered to routine diagnosis laboratory of Adnan Menderes University, Faculty of Veterinary Science, Microbiology Departments to analyze them under cold chain and in terms of S. agalactiae. For S. agalactiae isolation purposes, double inoculation of sheep blood agar of each milk sample to be analyzed has been applied. Inoculated milk samples have been left for inoculation during 24-48 h at 37°C in both aerobic and micro aerobic medium. After incubation, gram staining has been applied to milk samples shaped, gram positive cocci have been sorted and catalyze test has been applied to gram positive cocci. Catalyze negative colonies have been separated to which other chemical tests have been applied. At the end of analysis, S. agalactiae has been identified in 12 of 116 goats samples (10.3%).
  Ilker Serin , Ahmet Ceylan , Sukru Kirkan and Ugur Parin
  In this study, twenty clinically healthy male camels (Camelus dromedarius) wrestling in traditional competitions were used to investigate preputial aerobic bacterial flora and antibiotic susceptibility. The aerobic bacteria included Serratia liquifaciens, Staphylococcus aureus, Streptococcus sp., Klebsiella ozaenae, Pseudomonas sp., Shigella sp., Enterobacter cloaceae, Flavobacterium sp., Actinomyces sp., Acinetobacter sp., Acinetobacter calcoaceticus and Bacillus sp. were the most common organisms and identified from the preputial samples. In this study, antibiotic susceptibility and resistance were also studied. All of the isolates that were identified in this study were susceptible to ciprofloxacine (100%), danofloxacine (89.2%) and resistant to cloxacillin (100%), sulbactam-ampicillin (85.7%) and cefuroxim sodium (64.2%).
  Sadik Buyukyoruk , Recep Cibik , Figen Cetinkaya , Gul Ece Soyutemiz , Ergun Omer Goksoy and Sukru Kirkan
  A total of 58 Village cheese samples produced from raw milk without using starter culture in Aydin province have been used for isolation of Lactococcus lactis isolates. For this purpose, a typical colony from each cheese sample representing lactococcus phenotype was confirmed by PCR using specific primers designed for conserved 16S rDNA regions. The number of bacteria growing on M17 agar plates containing nalidixic acid ranged from 1.1x107-1.7x109 cfu g-1 suggesting that lactococci is widespread in Village cheeses. Catalase activity, microscopy, growing at 10 and 45°C and in the presence of 2, 4 and 6.5% of NaCl were used for phenotypical characterization of isolates. By phenotypical characterization 49 isolates represented lactococcus profile. All isolates were then analyzed by PCR and 29 of them were identified as L. lactis sp. lactis, while only 4 were L. lactis sp. cremoris. About 16 isolates exhibiting lactococcal profile with phenotypic tests did not give any amplification band with the tested primers however, 2 isolates having atypical phenotype were identified as sp. lactis by PCR. The results of the present study suggest that Lactococcus is the widespread in the flora of village cheeses and would have important role in the formation of desired flavour and textural properties.
  Sukru KIRKAN , Osman KAYA , Serten TEKBIYIK and Ugur PARIN
  This study is aimed to detect Coxiella burnetii in cattle by Polymerase Chain Reaction (PCR). A total of 138 cattle blood samples were collected from 8 farms and then examined in laboratory conditions. The examination of Coxiella burnetii was carried out by PCR with specific primers. In this study, a total of 6 (4.3%) cattle serum samples were found PCR positive for Coxiella burnetii. This result proves that cattle are an important reservoir of Coxiella burnetii infection.
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