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Articles by Sukardi
Total Records ( 3 ) for Sukardi
  Ismoyowati , Sukardi and Agus Susanto
  This study was aimed to evaluate the genetic diversity based on phenotype and the genetic relationship between four kinds of Kedu chicken, using 4 microsatellite markers. The result from the phenotype observations showed that the four chicken breeds have qualitatively different plumage, skin, comb and shank colours. Cemani chickens’ have lower egg production than others. The results from the isolation and identification of DNA using microsatellite primers showed that the 4 primers were polymorphics. The highest polymorphic information contain values based on locus derived from the entire population was LEI 0147 (0.643), while the average polymorphic information contain value on each population were 0.362, 0.531, 0.482 and 0.568 for Cemani, white Kedu, red Kedu and black Kedu chickens, respectively. Estimation of the heterozygosity value on loci of different populations of Kedu chickens showed a large variation (0.618-0.743). Genetic distance analysis showed that among Kedu chickens had a genetic relationship ranging from 0.018 to 0.236. The conclusion was that the genetic diversity based on chicken phenotypes and based on microsatellite markers in the population of Kedu chickens indicated a high diversity and had a relatively distant genetic relationship.
  Sukardi , Sudjito Soeparman , Bambang Dwi Argo and Yudy Surya Irawan
  Patchouli (Pogostemon cablin, Benth) is an herbal plant and become a leading export commodity. Almost 80% of the world’s patchouli oil needs are supplied from the Indonesia. Patchouli oil is stored in the Glandular Trichomes (GT) which exist in all parts of the plant. The study aimed to determine the effects of pulsed electric field on the GT cells damage. The design of research was a completely randomized design and factorial treatments. The first study was conducted by voltages on 500-2000 V, exposure times of PEF 2 and 3 sec at a frequency of 500 Hz. The second study, treatment of 500-2000 Hz frequency, exposure times PEF 2 and 3 sec on the voltage of 500 V. The third study combined treatment mains voltages of 500, 750, 1000 and 2000 V, frequencies 500, 1000 and 2000 Hz and exposure times PEF 2 and 3 sec. The raw material in the form of fresh patchouli leaves the age of 7 months after planting and taken leaves of old. Data analysis is using software of SPSS 19 Version and to investigate the changes of GT cells used Scanning Electron Micrograph (SEM). The results showed that the number of GT cells on the lower surface of leaves (abaxial) and the upper surface of the leaf (adaxial) are 16-19 and 6-9 cells, respectively with a diameter of ±60-63 μm. In the area of 1 cm2, the number of GT cell on abaxial and adaxial leaves are ±800 and ±355 cells, respectively or mean number of GT cells on whole leaves is ±1.155/cm2. The electric field strength of PEF treatment has greater influence on GT cell damage than the frequency. PEF treatment by electric field strength of 125 V cm-1 makes GT cells patchouli damage. PEF frequency treatment makes GT cells wrinkled and PEF strength makes GT cell wall are erupted.
  Pandapotan Turnip , Hamidah Harahap , Sukardi , Nuim Hayat , Rosdanelli Hasibuan and Taslim
  Natural rubber latex waste filled with water hyacinth was used as adsorbent as a part of the waste alternative utilization. This study was aimed to determine the maximum contact time and pH of the adsorbent on the adsorption process of grease in water. It was carried out by contacting the adsorbent with the mixture of grease and water for 5-25 min with the addition of different pH solution. It was found that the adsorption capacity of the adsorbent was improved with the increasing contact time while the increase of pH solution showed no significant effect on the adsorption capacity.
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