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Articles by Sudjadi
Total Records ( 5 ) for Sudjadi
  Yanita IkaWidyasari , Sudjadi and Abdul Rohman
  Rat meat is not halal for Muslims, so that the presence of rat meat in any food is a crucial issue. The aim of this study was to design specific primer from Mitochondrial Cty b Rattus argentiventer that can be used for determining rat meat contamination or rat meat adulteration in meatball formulation using, Real Time Polymerase Chain Reaction (RT-PCR). The specificity of primers was confirmed in fresh tissue from pigs, cows, chickens, goats, rabbits and white mice. The designed primers were then used to analyze rat meat DNA in meatball formulation made from rat meat and beef mixture at 1, 2, 3, 5, 10, 25, 50 and 100% incorporation of rat meat. The repeatability test was performed by measuring the amplification from fresh rat tissue and rat meat in meatball. Primers were also subjected to sensitivity test of 6 dilution series (50000, 5000, 500, 50, 5 and 0.5 pg μL–1) of rat tissue. From two primers designed, primers cytb 42 (forward: 5’-TAA CCA CTC CTT CAT CGA CCT T-3’; reverse: 5’-CCC CGT TGG CGT GTA AAT A-3’) were more specific to evaluate the presence of rat meat in fresh tissue and in meatball formulation at optimum annealing temperature of 61.4°C. The primers can be used for DNA identification by RT-PCR with sensitivity expressed by limit of detection of 5 pg or in meatball formulation at concentration of 1% rat meat.
  Theresia Sepminarti , Sudjadi , Herllya Selvi Wardani and Abdul Rohman
  Currently, along with the development of science and technology, the diversification of food products occurs in the market. Food products can contain non-halal components like porcine gelatine. One of food suspected to use gelatine is soft candy. Gelatin can be made from pork or beef or other animal. The presence of porcine gelatine in any food products is not allowed for Moslem community, therefore an analytical method offering reliable results must be developed. This study is intended to use Real-Time Polymerase Chain Reaction (RT-PCR) for analysis of porcine gelatine in soft candy. Isolation of DNA was performed with mitochondrial DNA Isolation Kit K280-50 (Bio-Vision). The DNA was analyzed by RT-PCR using primer D-Loop 318. Analysis for the primer specificity was performed on fresh tissue (pig, cows, chickens, goats and rats) and gelatin sources (beef, pigs and catfish). Primer D-loop318 can amplify porcine DNA at the optimum temperature 61.4°C. Repeatability test demonstrated amplification of all positive response samples containing porcine DNA in serial dilution of 10000-1 pg). The Coefficient of Variation (CV) is 6.32%. The repeatability test was also performed on soft candy 100% having CV of 1.06%. The commercial soft candy samples evaluated do not contain porcine DNA.
  Abdul Rohman , Diana Silawati , Sudjadi and Sugeng Riyanto
  The objective of this study is to evaluate the capability of UV-spectrophotometry in combination of multivariate calibration based on Partial Least Square (PLS) regression for simultaneous quantitative analysis and dissolution evaluation of sulfamethoxazole (SUL) and trimethoprim (TRI) in tablet dosage form. The experimental calibration and validation matrixes were constructed with 20 and 10 samples, respectively. The concentration range considered was 1-16 μg mL–1 for both SUL and TRI. The absorbance data of the calibration standards were taken between 200-400 nm. For achieving the best calibration model, the related parameters were evaluated. The optimum number of factors was selected by using the cross-validation method. The evaluation of calibration model is relied on the coefficient of determination (R2) and Root Mean Square Error of Calibration (RMSEC). The coefficient of determination (R2) for the relationship between actual values and predicted values of SUL and TRI was higher than 0.99 indicating good accuracy of the developed method. The RMSEC values obtained were relatively low, namely 0.167% (SUL) and 0.279% (TRI), which indicate acceptable precision of analytical method. The accuracy of developed method was comparable to that of High Performance Liquid Chromatography (HPLC) method. The UV spectrophotometry in combination with PLS calibration model was successfully used for quantitative analysis and dissolution studies of SUL and TRI sulfamethoxazole and trimethoprim in tablet dosage form.
  Abdul Rohman , Yani Ardiyanti , Sudjadi and Sugeng Riyanto
  The method of choice for analysis of drugs in multi-component preparations is chromatographic based technique such as High Performance Liquid Chromatography (HPLC). However, chromatographic method is time consuming and requiring much effort. As a consequence, some simple methods such as UV spectrophotometry are continuously developed, especially in combination with the chemometrics software. The UV-vis spectrophotometry coupled with multivariate calibration of Partial Least Square (PLS) has been developed for quantitative analysis of paracetamol, guaiphenesin and chlopheniramine maleate in the presence of phenylpropanolamine without separation step. The calibration model is prepared by developing a series of sample mixture comprising these drugs in certain proportion. The evaluation of calibration model was based on coefficient of determination (R2) and Root Mean Square Error of Calibration (RMSEC). The result showed that UV spectrophotometry combined with PLS can be used for quantitative analysis of drugs. The coefficient of determination (R2) for the relationship between actual values and predicted values was higher than 0.99 indicating good accuracy. The RMSEC values obtained were relatively low indicating good precision. The accuracy of developed method was compared to that of HPLC. The developed method was successfully used for analysis of paracetamol, guaiphenesin and chlopheniramine maleate in tablet dosage form.
  Abdul Rohman , Sudjadi , Devi , Dwiky Ramadhani and Ardi Nugroho
  FTIR spectroscopy in combination with multivariate calibration of Partial Least Square (PLS) has been developed for quantification of curcumin in the ethanolic extracts of Curcuma longa Linn and Curcuma xanthorriza Roxb. The optimization was done by selecting the best wavenumbers regions capable of providing the high coefficient of determination (R2) and low values of Root Mean Square Error of Calibration (RMSEC). Finally, wavenumbers region of 2000-950 cm–1 was selected for prediction of curcumin in the extracts. The correlation between actual values of curcumin determined by HPLC and FTIR predicted values using FTIR spectroscopy combined with PLS in ethanolic extract of C. longa and C. xanthorriza at 2000-950 cm–1 revealed R2 values of 0.96 and 0.99, respectively. The RMSEC values obtained are 0.299 and 0.089 for C. longa and C. xanthorriza, respectively. The high value of R2 and low value of RMSEC indicated the high accuracy and precision of FTIR spectroscopy for quantification of curcumin in the extracts. These results indicated that FTIR spectroscopy combined with PLS is an alternative technique for determination of curcumin in Curcuma species. The developed method (FTIR spectroscopy) is rapid, no sample preparation and not involving excessive solvents and reagents.
 
 
 
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