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Articles by Sudarsono
Total Records ( 5 ) for Sudarsono
  Triadiati , S. Tjitrosemito , E. Guhardja , Sudarsono , I. Qayim and C. Leuschner
  Litterfall production, litter decomposition and nutrient released were influenced by land use change from natural forest into cacao agroforestry. Cacao agroforestry is a traditional form of agriculture practiced by the people of Central Sulawesi. The study was carried out in the cacao was planted under forest covers (CF1), under planted trees (CF2) and between shade trees (CP). The Natural Forest (NF) was used as the undisturbed ecosystem compared to cacao agroforestry. This research was carried out since March 2005-February 2006. We recorded litterfall production by using litter trap and decomposition of leaf litter by nylon net bag technique. Litterfall production in relation to climate was analyzed by periodic curve. The result showed that NF had the higher annual litterfall (13.67 t/ha/year) than that of cacao agroforestry. Monthly litterfall in NF and cacao agroforestry were influenced by climate. Natural forest had the highest coefficient of decomposition (k = 3.07 in March-June 2005 and 1.85 in September-December 2005) and or the fastest rate of decomposition, whereas cacao agroforestry under CF2 had the lowest one (k = 1.23 in March-June 2005 and 1.14 in September-December 2005). Carbon released was higher in the NF (1.1% in March-June 2005 and 0.5% in September-December 2005) than that of cacao agroforestry. Nitrogen released was higher in the NF than that of cacao agroforestry in the first period (1.1% in March-June 2005).
  Naniek Widyaningrum , Achmad Fudholi , Sudarsono and Erna P. Setyowati
  One of problem from anti acne cream formula containing Epigallocatechin gallate (EGCG) from green tea leaves is EGCG stability. The EGCG is unstable to heat and easily oxidated. However, EGCG is the main marker substance related to antibacterial activity. This study aimed to determine the EGCG stability and its antibacterial activity. The EGCG stability testing was obtained during extraction process by adding phosphoric acid buffer, pH 4 buffer solution and control. The stability of these groups then tested at 2, 25 and 40°C temperature. Evaluation for absorbance changing by UV-VIS spectrophotometry was obtained during 15 days. After that, EGCG concentration from the three groups were compared using HPLC. Antibacterial activity testing for acne causing bacteria Staphylococcus epidermidis ATCC 35984 was conducted from phosphoric acid and pH 4 buffer solution groups. The result showed that extreme cold temperature decreasing treatment against green tea leaves extract after infundation, pH 4 buffer solution adding and storage at temperature of 2°C produced the highest EGCG concentration namely 60.98% w/w. Besides that, antibacterial activity from the ethyl acetate fraction of green tea leaves extract by using this method produced a good inhibition against P. acnes and S. epidermidis. Moreover, the antibacterial activity against P. acnes was higher than S. epidermis. The adding of pH 4 buffer solution in extraction process produced the highest EGCG concentration than phosphoric acid adding and no buffer adding. The EGCG was stable at the storage temperature of 2°C and 6% ethyl acetate fraction of green tea leaves extract can be used as an anti acne dosage.
  Erna P. Setyowati , Umar A. Jenie , Sudarsono , L.B.S. Kardono and R. Rahmat
  Structure identification of cytotoxic of isolated compound from Kaliapsis sp. sponge collected from North west Bali sea was conducted. The identity of the structure was analyzed based on physical and spectral data, namely, ultraviolet, MS, one- and two-dimensional 1H-NMR and 13C-NMR and comparison to published values. The isolated compound was confirmed as Theonellapeptolide Id.
  Erna P. Setyowati , Umar Anggara Jenie , Sudarsono and L. Broto Kardono
  A study about cancer cell selectivity and apoptosis cell death had been conducted against 5M74 (theonellapeptolide 1d) and 1 (1-(tetrahydro-4-hydroxy-5-(hydroxymethyl)furan-2-yl)-5-methylpyrimidine-2,4 (1H,3H)-dione). The 5M74 and 1 were isolated from Kaliapsis sp., sponge from West Bali National Park Indonesia. Cell selectivity was observed using heLa cell, myeloma cell, Raji cell and T47D cell. Apoptosis cell was conducted against the most sensitive cell with both isolates. The result were the 1 isolate was the most cytotoxic against myeloma cell because it had the lowest IC50 value namely 0.18 μg mL–1 while 5M74 isolate had the lowest IC50 value against Raji cell (7.8 μg mL–1). The isolated cell death test result was the 1 isolate increased apoptosis against myeloma cell. Moreover, on 5M74 isolate also increased apoptosis against Raji cell.
  E. P. Setyowati , U. A. Jenie , Sudarsono , L.B. S. Kardono and R. Rahmat
  Identification of cytotoxic constituent of Indonesian sponge Kaliapsis sp. has been conducted. The structure identification was judged based on the spectroscopic data, namely, ultraviolet, MS, one and two-dimensional 1H-NMR and 13C-NMR methods. The cytotoxic constituent was identified as 1-(tetrahydro-4-hydroxy-5-(hydroxymethyl)furan-2-yl)-5-methyl pyrimidine-2,4(1H,3H)-dione. This constituent hasn’t been isolated from sponges as natural product.
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