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Articles by Sudagar Mohamad
Total Records ( 14 ) for Sudagar Mohamad
  Sudagar Mohamad and Hajibeglou Abasali
  Ten plants were extracted with 70% ethanol and screened for their antimicrobial activities against Aeromonas hydrophila, a bacterial pathogen. Using disk diffusion assays, five extracts among these ten (Inula helenium, Tussilago farfara, Brassica nigra, Echinacea purpurea and Chelidoniume majus) were selected and equal proportions of them were mixed thoroughly with the artificial feeds at concentrations of 0.0 (A), 100 (B), 250 (C), 500 (D), 750 (E) and 1000 (F) mg kg-1 of dry diet. The prepared diets were fed to healthy common carp (Cyprinus carpio) for 60 days and then challenged with A. hydrophila. To evaluate the immune response and resistance against A. hydrophila infection of fish, haematological, biochemical and immunological parameters of them were investigated at 20, 40 and 60 days of feeding and also 10th day post-challenge. Results indicated that respiratory burst activity, serum bactericidal activity, lysozyme, serum protein, albumin, globulin, WBC, RBC and haemoglobin content were enhanced (p<0.05) in fish fed herbal diets compared to the control group. On 10 days post-challenge, the total survival rates were 38.09% in control group (A) and 61.90, 67.62, 77.14, 85.71 and 80.95% in group B, C, D, E and F, respectively. Among different groups, E generally showed the best performance in the experiment. Further research is needed to isolate and characterize the active compounds from these plants.
  Hajibeglou Abasali and Sudagar Mohamad
  This study was conducted to evaluate the immunostimulatory and growth promoting effects of four different herbal medicinal plants on non-specific immune response and resistance to Aeromonas hydrophila challenge of common carp, C. carpio. The ethanol extract of 11 plants were screened for their antimicrobial activity against A. hydrophila, a bacterial pathogen. Of these, 4 plant extracts, Ocimum basilicum, Cinnamomum zeylanicum, Juglans regia and Mentha piperita were selected and mixed thoroughly in equal proportion. The mixed extract was incorporated with the artificial feeds at concentration of 0.0 (A), 250 (B), 500 (C), 750 (D), 1000 (E) and 1250 (F) mg kg-1 of dry diet and fed to healthy fish. After 45 days of feeding, a challenge trial was conducted by injection of A. hydrophila for 10 days. Every 15 days and also at 10th day post-challenge, immunological, biochemical and haematological parameters of fish were studied. Results indicated that fish fed with herbal immunostimulants diets showed enhanced bactericidal activity, serum lysozyme, respiratory burst activity, WBC, RBC, haemoglobin, total serum protein, albumin and globulin compared to the control group (p<0.05). As the value of herbal extracts was increased in diets, the plasma glucose level decreased. The mortality was recorded up to 10 days post-challenge. All experimental groups showed higher survival rate compared to control (p <0.05). The survival percentage was found highest (91.42%) in the group E and lowest (48.58%) in control group. It can be conducted that the plant extracts we used in this study can act as immunostimulants, enhance the non-specific immunity and increase disease resistance of common carp, C. carpio to A. hydrophila infection.
  Hajibeglou Abasali and Sudagar Mohamad
  A commercial probiotic, Primalac was incorporated in fish feed at four different concentrations of 0.0, 0.04, 0.09 and 0.14% and fed to platy Xiphophorus maculatus for a period of 26 weeks to observe the effect of dietary probiotic supplementation on their reproductive performance. Results indicated that female broodstock fed the diets supplemented with primalac showed enhanced Gonadosomatic Index (GSI) and fry production compared to the control group (p<0.01). There were no significant differences in the percentage of deformed fry and fry weight among the dietary treatments (p>0.05). The results also showed that fecundity, fry survival (%) and length of fry were significantly (p<0.01) higher in experimental groups (except the group fed 0.04% Primalac mixed diet) compared to the control group (p<0.01). It can be conducted that the probiotic Primalac we used in this study can improve the reproductive performance of female platy-fish broodstocks during reproductive stages.
  Hajibeglou Abasali and Sudagar Mohamad
  To determine the effect of immunogen as a commercial prebiotic on the reproductive performance of a freshwater ornamental species, swordtail (Xiphophorus helleri), fish were fed diets containing four doses of immunogen viz. 0% (A), 0.5% (B), 1% (C) and 1.5% (D) for 26 weeks. Results showed that all experimental groups showed higher fry survival (%), relative fecundity and fry production compared to control (A) (p<0.01). Weight and length of fry were significantly higher in group C and D as compared to other groups (p<0.01). The Gonadosomatic Index (GSI) was found highest in group C and lowest in control group. The results also showed that the percentage of deformed fry was significantly higher in control group as compared to other groups. Collectively, this study showed that reproductive performance of female swordtail can be improved by dietary prebiotic immunogen supplementation. Further study is needed into the mechanism (s) of action for prebiotics such as immunogen and their application in aquaculture.
  Hajibeglou Abasali and Sudagar Mohamad
  This study was conducted to evaluate the effect of dietary prebiotic immunogen on the reproductive performance of a livebearing ornamental species, platy (Xiphophorus maculatus). Fish were fed under four regimes, 0% (A), 0.5% (B), 1% (C) and 1.5% (D), immunogen mixed diet for a period of 26 weeks. Results indicated that Gonadosomatic Index (GSI), fry production, relative fecundity and fry length were significantly (p<0.01) higher in group D as compared to the control (group A) and other experimental groups. The percentage of deformed fry was not significantly affected by the various dietary treatments (p<0.05). Moreover, fry survival (%) was found lowest in control group. With the observed results, we are able to conclude that female platy fish broodstocks benefit from inclusion of prebiotic immunogen in diet during their reproductive stages.
  Hajibeglou Abasali and Sudagar Mohamad
  This experiment was conducted to evaluate the immunostimulatory and growth promoting effects of four different herbal medicinal plants on non-specific immune response and resistance to Aeromonas hydrophila challenge of common carp, C. carpio. The ethanol extract of 11 plants were screened for their antimicrobial activity against A. hydrophila, a bacterial pathogen. Of these, 4 plant extracts, Ocimum basilicum, Cinnamomum zeylanicum, Juglans regia and Mentha piperita were selected and mixed thoroughly in equal proportion. The mixed extract was incorporated with the artificial feeds at concentration of 0.0 (A), 250 (B), 500 (C), 750 (D), 1000 (E) and 1250 (F) mg kg-1 of dry diet and fed to healthy fish. After 45 days of feeding, a challenge trial was conducted by injection of A. hydrophila for 10 days. Every 15 days and also at 10th day post-challenge, immunological, biochemical and haematological parameters of fish were studied. Results indicated that fish fed with herbal immunostimulants diets showed enhanced bactericidal activity, serum lysozyme, respiratory burst activity, WBC, RBC, haemoglobin, total serum protein, albumin and globulin compared to the control group (p<0.05). As the value of herbal extracts was increased in diets, the plasma glucose level decreased. The mortality was recorded up to 10 days post-challenge. All experimental groups showed higher survival rate compared to control (p< 0.05). The survival percentage was found highest (91.42%) in the group E and lowest (48.58%) in control group. It can be conducted that the plant extracts we used in this study can act as immunostimulants, enhance the non-specific immunity and increase disease resistance of common carp, C. carpio to A. hydrophila infection.
  Hajibeglou Abasali and Sudagar Mohamad
  This experiment was conducted to test the Valeriana officinalis root extract during transportation of swordtail, Xiphophorus helleri. For the transport experiment, fish were distributed in 24 plastic bags, of which 12 contained the V. officinalis root extract (1 g L) (group B) and the remaining had no herbal extract and acted as control (group A). Transport lasted 24 h and every 6 h, 3 bags of each treatment were sampled. Survival, water quality parameters and whole-body cortisol were then studied. Results indicated that no significant differences (p>0.05) in the dissolved oxygen, pH, temperature and ammonia levels were observed between treatments at all the assay periods. Whole-body cortisol levels in group B was significantly (p<0.05) lower than control group. The survival rate (%) was significantly (p<0.05) higher in group B compared to the control group. The present research revealed that the application of V. officinalis root extract will be effective in suppressing stress responses in X. helleri and it can be recommended for ornamental fish transport.
  Hajibeglou Abasali and Sudagar Mohamad
  A commercial probiotic, Primalac was incorporated in fish feed at four different concentrations of 0.0, 0.04, 0.09 and 0.14% and fed to platy Xiphophorus maculatus for a period of 26 weeks to observe the effect of dietary probiotic supplementation on their reproductive performance. Results indicated that female broodstock fed the diets supplemented with Primalac showed enhanced Gonadosomatic Index (GSI) and fry production compared to the control group (p<0.01). There were no significant differences in the percentage of deformed fry and fry weight among the dietary treatments (p>0.05). The results also showed that fecundity, fry survival (%) and length of fry were significantly (p<0.01) higher in experimental groups (except the group fed 0.04% Primalac mixed diet) compared to the control group (p<0.01). It can be conducted that the probiotic Primalac we used in this study can improve the reproductive performance of female platy-fish broodstocks during reproductive stages.
  Hajibeglou Abasali and Sudagar Mohamad
  To determine the effect of Immunogen as a commercial prebiotic on the reproductive performance of a freshwater ornamental species, swordtail (Xiphophorus helleri), fish were fed diets containing four doses of Immunogen viz. 0 (A), 0.5 (B), 1 (C) and 1.5% (D) for 26 weeks. Results showed that all experimental groups showed higher fry survival (%), relative fecundity and fry production compared to control (A) (p<0.01). Weight and length of fry were significantly higher in group C and D as compared to other groups (p<0.01). The Gonadosomatic Index (GSI) was found highest in group C and lowest in control group. The results also showed that the percentage of deformed fry was significantly higher in control group as compared to other groups. Collectively, this study showed that reproductive performance of female swordtail can be improved by dietary prebiotic Immunogen supplementation. Further study is needed into the mechanism (s) of action for prebiotics such as Immunogen and their application in aquaculture.
  Sudagar Mohamad and Hajibeglou Abasali
  Ten plants were extracted with 70% ethanol and screened for their antimicrobial activities against Aeromonas hydrophila, a bacterial pathogen. Using disk diffusion assays, five extracts among these ten (Inula helenium, Tussilago farfara, Brassica nigra, Echinacea purpurea and Chelidoniume majus) were selected and equal proportions of them were mixed thoroughly with the artificial feeds at concentrations of 0.0 (A), 100 (B), 250 (C), 500 (D), 750 (E) and 1000 (F) mg kg-1 of dry diet. The prepared diets were fed to healthy common carp (Cyprinus carpio) for 60 days and then challenged with A. hydrophila. To evaluate the immune response and resistance against A. hydrophila infection of fish, haematological, biochemical and immunological parameters of them were investigated at 20, 40 and 60 days of feeding and also 10th day post-challenge. Results indicated that respiratory burst activity, serum bactericidal activity, lysozyme, serum protein, albumin, globulin, WBC, RBC and haemoglobin content were enhanced (p<0.05) in fish fed erbal diets compared to the control group. On 10 days post-challenge, the total survival rates were 38.09% in control group (A) and 61.90, 67.62, 77.14, 85.71 and 80.95% in group B, C, D, E and F, respectively. Among different groups, E generally showed the best performance in the experiment. Further research is needed to isolate and characterize the active compounds from these plants.
  Hajibeglou Abasali and Sudagar Mohamad
  This experiment was conducted to test the Valeriana officinalis root extract during transportation of swordtail, Xiphophorus helleri. For the transport experiment, fish were distributed in 24 plastic bags of which 12 contained the V. officinalis root extract (1 g L-1) (group B) and the remaining had no herbal extract and acted as control (group A). Transport lasted 24 h and every 6 h, 3 bags of each treatment were sampled. Survival, water quality parameters and whole-body cortisol were then studied. Results indicated that no significant differences (p>0.05) in the dissolved oxygen, pH, temperature and ammonia levels were observed between treatments at all the assay periods. Whole-body cortisol levels in group B was significantly (p<0.05) lower than control group. The survival rate (%) was significantly (p<0.05) higher in group B compared to the control group. The present research revealed that the application of V. officinalis root extract will be effective in suppressing stress responses in X. helleri and it can be recommended for ornamental fish transport.
  Hajibeglou Abasali and Sudagar Mohamad
  A study to determine the effect of varying dietary probiotic levels on the reproductive performance of a freshwater ornamental species, the swordtail (Xiphophorus helleri) was carried out. The commercial probiotic, Primalac were mixed thoroughly with the artificial feeds at concentration of 0.0% (A), 0.04% (B), 0.09% (C) and 0.14% (D) and fed to healthy fish for a period of 26 weeks. Results showed that supplementation of feed with probiotic increased significantly (p<0.05) the Gonadosomatic Index (GSI), fecundity and fry production of female broodstock. Fry production, fecundity and fry survival were significantly higher in group B as compared to the group A (control) (p<0.05). The highest significant Gonadosomatic Index (GSI) and fry weight were observed in group B (p<0.01). The fish of experimental groups B and A recorded the lowest and highest deformed fry (%), respectively. Based on these data, it is concluded that female swordtail broodstocks benefit from inclusion of primalac in diet during their reproductive stages. Further study in needed into the mechanism (s) of action for probiotics such as Primalac and their application in aquaculture.
  Hajibeglou Abasali and Sudagar Mohamad
  A commercial probiotic, Primalac was incorporated in fish feed at four different concentrations of 0.0, 0.04, 0.09 and 0.14% and fed to platy Xiphophorus maculatus for a period of 26 weeks to observe the effect of dietary probiotic supplementation on their reproductive performance. Results indicated that female broodstock fed the diets supplemented with Primalac showed enhanced Gonadosomatic Index (GSI) and fry production compared to the control group (p<0.01). There were no significant differences in the percentage of deformed fry and fry weight among the dietary treatments (p>0.05). The results also showed that fecundity, fry survival (%) and length of fry were significantly (p<0.01) higher in experimental groups (except the group fed 0.04% Primalac mixed diet) compared to the control group (p<0.01). It can be conducted that the probiotic Primalac we used in this study can improve the reproductive performance of female platy-fish broodstocks during reproductive stages.
  Hajibeglou Abasali and Sudagar Mohamad
  This study was conducted to evaluate the effect of dietary prebiotic Immunogen on the reproductive performance of a livebearing ornamental species, platy (Xiphophorus maculatus). Fish were fed under 4 regimes, 0% (A), 0.5% (B), 1% (C) and 1.5% (D) Immunogen mixed diet for a period of 26 weeks. Results indicated that Gonadosomatic Index (GSI), fry production, relative fecundity and fry length were significantly (p<0.01) higher in group D as compared to the control (group A) and other experimental groups. The percentage of deformed fry was not significantly affected by the various dietary treatments (p<0.05). Moreover, fry survival (%) was found lowest in control group. With the observed results, researchers are able to conclude that female platy-fish broodstocks benefit from inclusion of prebiotic immunogen in diet during their reproductive stages.
 
 
 
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