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Articles by Suchitra Sanasam
Total Records ( 3 ) for Suchitra Sanasam
  Debananda S. Ningthoujam , Suchitra Sanasam and Salam Nimaichand
  Problem statement: The exhaustion of the usual terrestrial sources and the rise of resistant pathogens dictate the search for novel actinomycetes and new antibiotics. In this context, niche habitats such as caves, pristine forests, lakes, rivers, and other wetlands, high salt environments, marine ecosystems and endophytic niches are promising targets for survey of bioactive actinomycetes. Approach: Actinomycetes were isolated from several niche habitats in Manipur, India, on selective media such as SCNA and Chitin agar with or without antibiotics. Selected isolates were subjected to antimicrobial activity screening by Kirby-Bauer method. Results: 172 lake sediment (SCNA, LS1 series), 35 lake sediment (CA, LSCH series), 120 river (NRP, NRB and..series), 39 forest (AML series), 35 cave (KC1 series), 101 salt spring (NH, N3S and .. series), 46 Shirui jungle (SJ series) and 66 Shirui hill (SH series) actinomycetes isolates were obtained. Of 99 randomly selected isolates screened, 37 had antimicrobial activities against 1 or more indicator strains: 32 against Gram positive bacteria and 8 against Gram negative bacteria; 10 actinomycete strains were antimycotic and 3 had broad-spectrum antibiotic activities. About 18 potent antibacterial, 1 anti pseudomonas, 1 exclusively antifungal and 3 broad-spectrum antimicrobial actinomycetes were chosen for further studies. Conclusion: Niche habitats in Manipur especially wetlands show great promise for discovery of bioactive actinomycetes.
  Suchitra Sanasam and Debananda S. Ningthoujam
  The present study was aimed at screening of local actinomycete isolates for anticandidal activity, selection and characterization of potent strain (s) and initial optimization of fermentation conditions for anticandidal production. Of 35 actinomycete isolates in our collection from various biotopes in Manipur that were screened by cross-streak method, 2 strains (5.7% of isolates screened) NRP1-14 and NRP1-26, obtained from Nambul river in Imphal, Manipur, India, showed anticandidal activity. Based on relative potency, NRP1-14 was selected for further studies. It has been identified as Streptomyces mutabilis NRP1-14 based on phenotypic and genotypic characteristics. Among various media tested for submerged fermentation of the bioactive strain, Starch Casein Nitrate (SCN) broth was found to be the best. The strain produced significant activity against Candida albicans MTCC 227 in SCN broth after 3 days of incubation in SCN broth of pH 7.2 at 28°C under shaking speed of 150 rpm. The NRP1-14, interestingly, also exhibited significant antagonistic activity against several rice fungal pathogens-Bipolaris oryzae, Curvularia oryzae, Fusarium oxysporum and Pyricularia oryzae. Anticandidal activity is being optimized by improving cultural and environmental conditions for fermentation. This study shows that biotopes in Manipur can yield promising actinomycetes for use in medicine and agriculture. To our knowledge, this is the first report of a Streptomyces mutabilis strain with potent anticandidal activity. The NRP1-14, therefore, warrants further investigation for possible application as anticandidal and biocontrol agents (BCAs).
  Debananda Ningthoujam , Suchitra Sanasam and Salam Nimaichand
  A Streptomyces sp. LS1-128 (closely related to S. sindenensis) isolated from the sediments of Loktak Lake, the largest freshwater lake in Eastern India, shows broad spectrum antimicrobial activity with significant inhibition zones against B. pumilus MTCC 1607 (25 mm), E. coli MTCC 739 (20 mm), B. sphaericus MTCC 2303 (19 mm), B. subtilis MTCC 121 (14 mm) and C. albicans MTCC 227 (11 mm). LS1-128 is an aerobic, gram-positive actinomycete with off-white to brown substrate mycelium with no diffusible pigment. Spores are arranged in rectiflexible chains in the aerial mycelium. It grows well in ISP2, ISP3, ISP4, ISP5, ISP6, ISP7, Streptomyces Agar and Actinomycete Isolation Agar. It can degrade starch, casein and urea and has good growth on almost all carbon sources tested including L-Arabinose, D-Fructose, D-Galactose, D-Glucose, D-Mannitol, D-Rhamnose, Salicin and D-Xylose. Based on the phenotypic and genotypic characteristics, LS1-128 was found to be most closely related to Streptomyces sindenensis (99.927% homology). The other close relatives are S. parvus, S. badius and S. globisporus). LS1-128 with promising antimicrobial activities obtained from Loktak Lake sediment has been putatively designated as Streptomyces sindenensis strain LS1-128.
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