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Articles by Subhash Chandra
Total Records ( 6 ) for Subhash Chandra
  Sonam Sneha , Anirudha Rishi and Subhash Chandra
  In this study the 21 days old seedlings of Pennisetum glaucum were subjected to short term salt stress in order to observe the effect on chlorophyll content, protein and antioxidative enzyme activity (CAT and APX) responses. The aim of the experiment was to evaluate the changes in chlorophyll content and antioxidant enzymes (Catalase and Ascorbate peroxidase). The 21 days old seedlings were subjected to salt stress by supplementing Hoagland’s solution with different concentrations of Sodium chloride (50, 100, 150 and 200 mM). Measurement of chlorophyll content and antioxidant enzyme activity were taken at different time intervals of salt treatment (12, 24, 48, 72, 96 and 120 h). The results showed statistically significant differences in traits for the salt treatment. A significant increase in Catalase activity was observed under all the salt concentrations while no significant activity of Ascorbate peroxidase activity was observed. The protein content and chlorophyll content decreased with increasing salt concentration. This result shows salt stress affects the photosynthesis rate by decreasing chlorophyll content. Catalase enzyme plays an important role in scavenging reactive oxygen species generated due to salt stress in the plant cell.
  Nakuleshwar Dut Jasuja , Richa Saxena , Subhash Chandra and Richa Sharma
  Punica granatum L. commonly known as Pomegranate belongs to the Family Punicaceae. Pomegranate has been known to be a reservoir of secondary metabolites which are being exploited as source of bioactive substance for various pharmacological purposes. Many researchers have focused their interest to investigate the bioactive compounds of Punica granatum for human health. In order to facilitate the further investigation and exploit the said plant, this study summarized herein the research achievements on some of the phytochemical and pharmacological properties of Punica granatum. The different types of phytochemicals have been identified from various parts of the pomegranate tree and from pomegranate juice, leaves, flower, fruits and seeds. The constituents of this plant were thoroughly reviewed discussed based on literatures.
  Nakuleshwar Dut Jasuja , Richa Saxena , Subhash Chandra , Shilpa Bhargava and S.C. Joshi
  Plant derived medicinal products have been used for centuries in every culture throughout the world. Mimosa hamata possesses a vast ethnomedicinal history of heuristic medical value. Ethnomedicinal use of Mimosa hamata has been known since time immemorial and this plant were used to cure diseases and to maintain good health. M. hamata whole plant are used in traditional systems of medicine for treating various diseases. M. hamata exhibited higher antioxidant, antibacterial, antifungal and antiviral activities. Some bioactive constituents of this plant were thoroughly reviewed and discussed based on literatures. M. hamata has been claimed as folk medicinal plant but little is known about the phytochemicals and pharmacognostical information. There is a need to review this plant in order to provide scientific information for its application in traditonal and biological medicinal system.
  Avinash Marwal , Surendra Meena , Subhash Chandra and Anima Sharma
  Dermatophytes as the name suggest are the fungus that feed on skin. The chief source of their growth is keratin which is widely available in skin, nails and hairs. Here we have evaluated an In vitro study of antidermatophytic activity of Mint (Mentha piperita) against two dermatophytes i.e., Trichophyton rubrum and Microsporum canis. The data in the manuscript is very much helpful in curing dermatophytic infections as an application from Biotechnological point of view. Distribution of Trichophyton rubrum and Microsporum canis was found to be 19.23 and 32.69%, respectively. At variable temperature Trichophyton rubrum and Microsporum canis showed maximum growth at 37°C (0.23 and 0.19 g dry weight of mycelium, respectively). At variable pH Trichophyton rubrum showed maximum growth at pH 7.5 and 8.0 (0.32 g dry weight of mycelium) and Microsporum canis showed maximum growth at pH 7.5 (0.39 g dry weight of mycelium). Mentha piperita highest keratinase activity against Microsporum canis was found to be 2.99 unit mL-1 with 2.91 mg mL-1 extracellular release of protein and in Trichophyton rubrum it was found to be 2.99 unit mL-1 with 2.75 mg mL-1 extracellular release of protein.
  Sonam Sneha , Anirudha Rishi , Amit Dadhich and Subhash Chandra
  Salinity is a major threat to agriculture, plants exhibits a variety of responses to salt stress that enable them to tolerate and survive in such conditions. Salinity affects physiological and biochemical processes in plants. A short term salt stress induced physiological and biochemical response were observed in P. glaucum. The experiment was conducted to understand the influence of salinity on seed germination, proline and free amino acid accumulation in P. glaucum. It was observed that as the salt concentration increased the germination percentage decreased as compared to control as well as the root/shoot length also decreased. This suggests that salinity greatly influences the germination as well as the plant growth. The levels of biochemical components proline and free amino acid were measured during the salt stressed condition. The 14 days old seedlings were subjected to 4 salt treatments (50, 100, 150 and 200 mM NaCl), free proline and free amino acids was calculated at 0, 12, 24, 48, 72 and 96th hour. Proline and free amino acid content in the salt stressed tissues increased with increase in salt concentration as well as with duration of salt stress. This result suggests that proline and free amino acid acids acts as compatible solutes in P. glaucum to protect the cellular macromolecules, maintain the osmotic balance and also scavenge the free radicals under salt stressed condition.
  Kriti Singh and Subhash Chandra
  Bioremediation play key role in the treatment of petroleum hydrocarbon contaminated environment. Exposure of petroleum hydrocarbon into the environment occurs either due to human activities or accidentally and cause environmental pollution. Petroleum hydrocarbon cause many toxic compounds which are potent immunotoxicants and carcinogenic to human being. Remedial methods for the treatment of petroleum contaminated environment include various physiochemical and biological methods. Due to the negative consequences caused by the physiochemical methods, the bioremediation technology is widely adapted and considered as one of the best technology for the treatment of petroleum contaminated environment. Bioremediation utilizes the natural ability of microorganism to degrade the hazardous compound into simpler and non hazardous form. This paper provides a review on the role of bioremediation in the treatment of petroleum contaminated environment, discuss various hazardous effects of petroleum hydrocarbon, various factors influencing biodegradation, role of various enzymes in biodegradation and genetic engineering in bioremediation.
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