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| Articles
by
Subagus Wahyuono |
Total Records (
2 ) for
Subagus Wahyuono |
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Syamsudin
,
Soesanto Tjokrosanto
,
Subagus Wahyuono
,
Darmono
and
Mustofa
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In vitro and in vivo antiplasmodial activities of stem barks extracts of Garcinia parvifolia Miq, medicinal plant traditionally used to treat malaria in Indonesia, have been evaluated. The IC50 value of extracts was determined. Extracts of this plant (n-hexane, ethylacetate and n-buthanol extracts) were tested in vitro for their antiplasmodial activity on 2 strains of Plasmodium falciparum, FCR-3 (chloroquine-resistant strain) and 3D7 (chloroquine-sensitive strain) using a visual method. Cytotoxicity of these extracts were performed on HeLa cells in vitro using MTT method. The in vivo antiplasmodial testing used a standard 4-days test on P. berghei infected mice and acute toxicity testing on mice were also conducted. The IC50 values of the plant extracts were in the range of 4,83-40,10 µg mL 1. Significant in vitro antiplasmodial activity and higher selective toxicity were observed by n-hexane extract (IC50 = 4,11 µg mL 1 and C/A = 48,23). The n-hexane extract was the most active in vivo against P. berghei (ED50 = 19,95 mg kg 1BW) and least toxic in mice (LD50= 1060,47 mg kBW 1). These results suggest that n-hexane extract is promising extract for further investigation for new antimalarial agents.
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Syamsudin
,
Soesanto Tjokrosonto
,
Subagus Wahyuono
and
Mustofa
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The study of in vivo antiplasmodial activity and acute toxicity of the active fraction of Garcinia parvifolia Miq. has been conducted. The fraction was obtained by maceration of n-hexane extract with methanol. A standard 4-day test on P. berghei infected Swiss mice was used to evaluate the in vivo antiplasmodial activity after an oral administration of the fraction in series dose of 25 to 200 mg kg-1 b.wt. once daily for 4 consecutive days. The in vivo antiplasmodial activity was expressed by the dose inhibiting 50% of parasite growth (ED50). Acute toxicity was evaluated in Swiss mice after oral administration of the active fraction in series dose of 2000 to 8000 mg kg-1 b.wt. The acute toxicity was expressed by the dose leading 50% deaths (LD50). The results showed that the active fraction of G. parvifolia Miq. was active against P. berghei in mice with an ED50 of 74.45 mg kg-1 b.wt. day-1. In addition, the active fraction was also relatively safe as expressed by the LD50 of 8000 mg kg-1 b.wt. |
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