Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Suaad S. Alwakeel
Total Records ( 4 ) for Suaad S. Alwakeel
  Suaad S. Alwakeel and Laila A. Nasser
  The occurrence of harmful aflatoxins from agricultural products varies with geographic location, farming practices and processing. To date, no data was reported from Saudi Arabia on mycotoxin content of nuts and edible seeds. Forty samples of edible nuts and dried seeds were randomly collected from different locations in Al-Riyadh, Saudi Arabia. Fungi were detected by seed-plate and dilutions plate method and were cultured on glucose-Czapek's agar, sucrose-Czapek's agar and starch yeast agar. Purified fungal isolates were identified morphologically. Mycotoxins were extractedusing chloroform and detected by thin layer chromatography. Bacterial analysis was done using total plate count method. There was a predominance of A. niger and A. flavus in all medium types. Aflatoxin B1 (8.5 μg mL-1) was detected in peanuts containing A. flavus. Aflatoxin B1 (1.7 μg mL-1) and B2 (1.7 μg mL-1) was detected in sunflower seeds containing A. terreus. T2 toxin (2.8 mg mL-1) was detected in pumpkinseeds containing Stachybotrys chartarum and DAS (2.4 μg mL-1) was detected in a salted peanut sample containing Trichthecium roseum. Four nut samples showed contamination with bacteria. Turkish pine seeds and American walnut had total plate counts of 12x10. Pakistani pine seeds and Iranian salted pistachio had TPC of 3x10. Listeria monocytogenes was isolated from American walnut samples. Government authorities for food safety consumption should continue to monitor and set appropriate guidelines and information initiatives for public knowledge on the safety of these agricultural products whole year round.
  Suaad S. Alwakeel and Laila A. Nasser
  Paper currency is used in exchange for goods and services and so the circulation of paper currency from one individual to another potentially spreads microorganisms. If pathogenic bacteria contaminate these currencies, the rate of infection and death rate from these infectious agents will continue to rise. This study was conducted to survey the bacterial and fungal contamination of paper money and cellular phones samples in Riyadh, Saudi Arabia in May 2010. Each bill and phone was prepared using standard procedures. Of the 390 currency notes, 282 (72.3%) were contaminated with bacteria which included Aspergillus niger, Aspergillus flavus, Candida spp., Penicillium spp. and Rhizopus spp. and bacteria which included Enterobacter cloacae, Klebsiella ozaenae, Cedecea davisae, Yersinia pseudotuberculosis, Acinetobacter iwoffii, Staphylococcus warneri and Enterobacter agglomerans. All isolated bacterial species were sensitive to ciprofloxacin, gentamicin, ticarcillin, tobramycin and trimethoprim-sulfamethoxazole. The use of commercial disinfectants was only effective against Aspergillus niger and Rhizopus spp. Cellular phones were contaminated with Micrococcus and Staphylococcus species and no fungal species were isolated from the sampled cellular phones. Prevention is the hallmark of reducing morbidity and mortality. An efficient health awareness campaign program should be fully implemented to inform the public of the hazards of contaminated paper currencies and even mobile phones.
  Suaad S. Alwakeel
  In this study, twenty five samples of well-known herbs in Riyadh, Saudi Arabia were collected and analyzed for Total Fungi Count (TFC). Mycotoxins were extracted and screened using SMKY liquid medium. One hundred and thirty adult female albino mice were grouped into three wherein one group (n =110) was fed with an aqueous extract from herbal plants. The second group (n =15) was fed with an aqueous extract of the isolated fungal species. The third group comprised the control group which was given water only (n =5). All mice were fed with mice breeding diet by Pillsbury, UK. After 5 weeks, mice were fasted and blood was withdrawn for biochemical analysis including alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyltransferase (GGT), serum creatinine and urea. Calligonum comosum with 2x105 cfu g-1 fungus spore, grained mixed herbs (24x103 cfu g-1) and Salvia officinalis (23x103 cfu g-1) were the most contaminated samples. The genus Aspergillus was the most dominant genus recovered (142 isolates) followed by Penicillium (14 isolates) and these two genera were found in 85.0 and 11.0% of the samples analyzed. Aspergillus fumigatus, Aspergillus flavus and Aspergillus ochraceus were the most dominant and frequently isolated (47.3, 46.5 and 18.1%, respectively), followed by Aspergillus citrinum (11.0%). Aspergillus ochraceus had 21.7 μg kg-1 of Aflatoxin B2 and 7.25 μg kg-1 of ochratoxin A, whereas Aspergillus flavus had 7.45 μg kg-1 of Aflatoxin B1 and Aspergillus fumigatus had 3.5 μg kg-1 of Aflatoxin B2 and 3.8 μg kg-1 of ochratoxin A. Mean creatinine, urea, ALT, AST and GGT were higher in mice fed or treated with herbal and fungal extracts group than the control group. This study confirms previous studies demonstrating the predominance of Aspergillus species in herbal and medicinal plants and its capability in the production of aflatoxin with induction of nephrotoxicity and hepatoxicity in animals and even in humans.
  Suaad S. Alwakeel and Eman Abdullah Hamad Al-Humaidi
  This study aimed to determine the clinically important levels of minerals in bottled fruit juices and drinks and to determine the microbial contamination of commercially available bottled fruit juices and drinks from different supermarkets in Riyadh, Saudi Arabia. Commercially available bottled fruit juices and drinks were brought from different supermarkets in Riyadh, were examined microbiologically and mineral contents were determined by atomic absorption spectrophotometry. A total of 150 specimens (3 replicates of a total 50 samples) were examined for microbial growth on six different culture media (BAP, NA, MacConkey, CAP, Salmonella Agar and PDA). A total of 43 (28.7%) different colonies were seen on different fruit juices. Bacillus cereus was the most common isolate in all types of fruit juices. Other isolates included Bacillus subtilis, Bacillus polymyxa, Chryseomonas luteola, Tatumella ptyseos, Streptococcus lactis and Candida sp. None of the specimens taken from softdrinks and power drinks showed any microbial growth after incubation for 48 h in all six environmental plates used. Specimens from mixed juice with milk showed microbial colonies in 3 out of 10 specimens with Lactobacillus sp., Streptococcus lactis and Lactobacillus casei. The mineral contents of 8 specimens of fruit juices had iron content within the maximum allowed concentration. As to potassium content, 7 of 8 (87.5%) of the samples had potassium content >10 ppm. Five of 8 (62.5%) samples had sodium content >20 ppm, 7 of 8 (87.5%) had aluminum content >0.2 ppm, 4 of 8 (50%) had lithium content >0.2 ppm, 7 of 8 (87.5%) had magnesium content >30 ppm, 4 of 8 (50%) had manganese content >20 ppm, all 8 contained lead >0.2 ppm and 7 of 8 (87.5%) have zinc content >5 ppm. Commercially sold fruit juices in Riyadh, Saudi Arabia should be further investigated and regulated since they contain dangerous organisms and minerals which are toxic to the body.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility