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Articles by Srihanam Prasong
Total Records ( 7 ) for Srihanam Prasong
  Srihanam Prasong
  This study was aimed to prepare SF/G blend films loaded tetracycline by a solvent evaporation method. Firstly silk cocoons were degummed and then dissolved by CaCl2:Ethanol:H2O (1:2:8 by mole). On another way, Gelatin (G) was prepared from gelatin powder. The mixture solution of SF/G ratios with 3:0, 2:1, 1:1, 1:2 and 0:3 were prepared before casting on the 5 cm polystyrene plates. All of films were investigated their morphology, chemical structures, thermal properties and percent transparency by using Scanning Electron Microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, Thermogravimetric Analyzer (TA) and UV-Vis spectrometer, respectively. The results found that the surfaces of blend films were gradually smooth without phase separation when the SF component decreased. The FTIR results of SF/G films showed strong regions for amide I, amide II and amide III which were the mixture characteristics of SF and G. The blend films rapidly decomposed in maximum rate after 300°C. The rate of weight lost depending on the content G meanwhile rapidly increased of weight lost when the G content was increased. The heat flow curves indicated that the blend films composed of multiple peaks of maximum decomposition temperatures as well as endo/exo-thermic. Finally, tetracycline could be interacted with G in excellent profile affected to the lowest of percent transparency.
  Srihanam Prasong
  The aims of this study were to prepare Silk Fibroin (SF)/Chitosan (CS) blend films loaded Methylene Blue (MB) and characterize their related properties. The SF was obtained from various Thai silk varieties locally called “Nang Lai, Mo and Kaki”. The blend films were prepared by mixing the SF, CS and MB solutions before pouring on polystyrene plates. They were then taken to an oven at 40°C for 3 days to obtain the films. In addition, each native SF silk variety and CS films were also prepared as control. SEM micrographs showed that native Nang Lai film appeared the bead like particles while other film types were smooth. Comparison between the blend films, Mo variety film has smooth surfaces while Nang Lai and Kaki films composed of flat particles covered their surfaces. FTIR results indicated that all of native SF has similar absorption bands at amide regions which coexisted of α-helix and β-sheet structures. Moreover, SF blended with CS showed strong bands at amide II, exhibit β-sheet structure. The blend film of Mo variety rapidly decomposed whereas Nang Lai was the lowest. In vitro releasing study indicated that MB released more quickly at the initial 30 min which Mo variety was the slowest.
  Simchuer Wilaiwan , Srisuwan Yaowalak , Baimark Yodthong and Srihanam Prasong
  This study aimed to prepare silk fibroin (SF)/gelatin (G) hybrid films by a solvent evaporation method for loading chlorhexidine diacetate (CHX). The SF and G solution in different ratios were mixed with CHX and placed on the 5 cm polystyrene plates before drying to obtain hybrid films. The films were determined their secondary structures and thermal properties by using Fourier transform infrared (FT-IR) spectrometer and thermogravimetric analysis (TGA), respectively. The results found that all of film composed of α-helix and β-sheet structures. However, differences of the α-helix and β-sheet structures were differed according to each component. The hybrid films showed soft texture and decreased of brittle compared to SF film only when the G content increased. Thermal properties of the films indicated that decomposition temperature profiles of all films did not differ dramatically, however, combination of characteristics both SF and G were appeared in hybrid films. The releasing rate of CHX-loaded in the films was found that the CHX has released from the SF film in higher rate than hybrid and G films, respectively. It is a promising that polarity, flexibility as well as component ratio of each polymer play important role on the releasing of CHX.
  Srihanam Prasong , Simchuer Wilaiwan and Srisuwan Yaowalak
  This study was aimed to study the surface morphology of Eri (Samia ricini) silk cocoons collected from different sources called China, Chaingmai, Lampoon, SIC (Silk Innovation Center) and Thai varieties. All of Eri silk fibers were prepared by hand. They were studied under scanning electron microscope (SEM) for morphological observation. The cross-section images showed China has composed of porous structures in higher ratios than Chaingmai and Lampoon, respectively. In contrast, SIC and Thai composed of nonporous structures. In addition, the effect of different organic solvents on each Eri silk fiber was also investigated. Eri silk fibers were treated with methanol, ethanol, ethyl acetate and methyl acetate in different incubation times and concentrations before examining with attenuated total reflection infrared (ATR-IR) spectroscopy. The results found that methanol showed the highest effect to change the structure of Eri fibers, compared to ethanol. Ethyl acetate and methyl acetate showed similar activity to enhance β-sheet form and lower than that of methanol and ethanol, respectively. The methanol concentrations and incubation times which suitable for China, Chaingmai, Lampoon, SIC and Thai were 40% (3 h), 40% (2 h), 60% (1 h), 60% (3 h) and 60% (3 h), respectively. From the results, SIC and Thai were considered as higher strength than those of China, Chaingmai and Lampoon. This was due to the fiber of the last group composed of porous structures in higher ratios than that of first group.
  Srihanam Prasong
  Antioxidant is an interesting molecule which has been studied world wide for human health. The aims of this study are to screen the Total Phenolic Content (TPC) and antioxidant activity of sericin extracts from 5 varieties of Eri silk. In addition, domesticated Thai silk; Nanglai and Dokbua were also investigated in comparison. The sericin was firstly extracted by warm distilled water. The TPC of each silk variety was determined using Folin-ciocalteu assay. The results indicated that domesticated silk have higher TPC than all of Eri. DPPH radical scavenging and Ferric Reducing/Antioxidant Power (FRAP) were used for measurement the antioxidant activity of the sericin extracts. The results found that domesticated Thail silk had higher antioxidant capacity than that of Eri but with different values. The results suggested that TPC and antioxidant of the sericin extracts exhibited good correlation. This means the TPC is main factor for antioxidant capacity. However, the variation of both TPC and antioxidant of sericin extracts were influenced by silk varieties. Moreover, domesticated Thai silk composed of some pigments which are antioxidant compounds. However, Eri silk should be considered as alternative sources for antioxidant.
  Ong-chiari Watcharin , Srisuwan Yaowalak , Simcheur Wilaiwan and Srihanam Prasong
  This study aimed to prepare Silk Fibroin (SF) and Gelatin (G) blend film and study its morphology, secondary structure and thermal properties compared to native SF and G films. The films were prepared from the SF solution by casting on the polystyrene plates. They were investigated their secondary structure by fourier transform-infrared (FTIR) spectroscopy, morphology using Scanning Electron Microscope (SEM). In addition, Thermogravimetric Analysis (TG) and Differential TG (DTG) were used for thermal properties investigation. The results found that the SF/G blend film composed of both α-helix and β-sheet structures which were similar characteristics of the native SF and G. This result was similar to the TG and DTG analysis according to blending between SF and G is not enhancing thermal stability of the film. However, changes in some absorption bands and temperatures were also observed from the blend film. The result suggested that chemical interaction and hydrogen bonding between SF and G could be formed. The formation could be affected to the uniform of the surface throughout the film under SEM.
  Srihanam Prasong and Tessanan Wasan
  Keratin solution was extracted from human hairs and used as subject for preparation of keratin/gelatin blend films. This study was aimed to explore the suitable method using for keratin extraction and extend to study the blend film properties. The blend films were prepared by simple evaporation method. After homogeneously mixed between keratin and gelatin solution at different ratios, the solution were placed on the plates and left in an oven at 40°C for 3 days. All of the films were then analyzed for their morphology, secondary structures and thermal properties by using SEM, FTIR and TGA, respectively. The result from SEM images showed that native keratin films have the highest rough surface compared to other films. In addition, the smooth surface of films gradually increased when the gelatin content increased. Keratin blending with gelatin showed structural changes, especially at the absorption bands of 3300-2900 cm-1 as well as the amide I, II and III regions. Moreover, thermal properties of the keratin films were enhanced by blending with gelatin. This study suggested that gelatin help to improve some properties of keratin while still remain its strength.
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