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Articles by Sri Harimurti
Total Records ( 5 ) for Sri Harimurti
  B. Ariyadi and Sri Harimurti
  Intestinal mucous containing mucins play an essential role as mucosal barrier to prevent invasion in the intestinal tissue of broilers. The aim of the study was to investigate the effect of supplementation of indigenous probiotics lactic acid bacteria on small intestinal histology structure and expression of mucins in the ileum of broiler chicken raised for 35 days. A total of 60 day old chick Lohmann strain broilers were randomly divided into four treatment groups, namely T0, T1, T2 and T3. The T0 group was raised with unsupplemented probiotics, while T1, T2 and T3 were orally supplemented multistrain probiotics at concentration 107, 108, 109 CFU/ml/bird/day, respectively. The results showed that supplementation of indigenous lactic acid bacteria probiotics significantly (P<0.05) increased villus height, villus width of duodenum, jejunum and ileum, as well as increased expression of mucin mRNA in the ileum compared to the control one in broilers. This results suggest that probiotics may stimulate proliferation of intestinal epithelium and regulate mucosal barrier formed by mucin in the intestine of broiler chickens.
  Charles V. Lisnahan , Wihandoyo , Zuprizal and Sri Harimurti
  Objective: The aim of this study was to determine the effect of the addition of methionine and lysine to feed based on cafeteria standards of native chickens on their growth performance (0-6 weeks). Materials and Methods: A total of 288 days-old native chickens (DOC) were used in this study. The DOC were divided into 4 treatments groups with 4 replications. The treatment diets were T0 and T1 (according to cafeteria and NRC standards) and T2 and T3, which were based on cafeteria standards with the addition of 0.14% methionine and 0.40% lysine for T2 and 0.27% methionine and 0.79% lysine for T3. The data collected were feed intake, body weight, feed conversion ratio and carcass as a percentage of body weight. Results: The results showed that the feed intake of T0, T1, T2 and T3 were 516.97, 556.91, 621.79 and 654.30 g/bird/6 weeks, respectively. The body weights for each group were 219.09, 232.67, 267.16 and 284.61 g/bird/6 weeks, respectively. The feed conversion data were 2.79, 2.80, 2.66 and 2.61, respectively and the carcass percentages were 53.20, 52.75, 54.63 and 56.85%, respectively. Conclusion: Feed formulated to cafeteria standards with the addition of 0.27% methionine and 0.79% lysine (group T3) resulted in the best growth performance.
  Cytske Sabuna , Wihandoyo , Sri Harimurti and R. Wisnu Nurcahyo
  Objective: The aim of this study was to quantify the bioactive components and antiparasitic activity of distillate citronella (Cymbopogon nardus or C. nardus) essential oil waste. Methodology: A densitometry method was used to quantitatively analyze the bioactive content of citronella waste. The effects of three concentrations of citronella waste extract and exposure time (30, 60, or 90 min) on adult Ascaridia galli (A. galli) worms in vitro from chicken intestines were evaluated. Results: Distillate waste from citronella (Cymbopogon nardus) (DWC) essential oil contains geraniol compounds. Treatment with DWC extract and powder significantly increased the mortality rate of A. galli compare to physiological solution (NaCl 0.9%). However, treatment with both 1.0% DCW and physiological solution (NaCl 0.9%) significantly increased the mortality rate of A. galli. Treatment effects increased significantly across all time points and there were interactions between material and concentration and; material and time points. Conclusion: The geraniol found in DWC may act as an antiparasitic against A. galli worms.
  Y. Laura , Sri Harimurti and Ismaya
  Objective: The objective of this study was to determine the effect of different dimethylacetamide (DMA) levels on sperm quality of Bangkok rooster chicken and sperm survivability in reproductive tract of hen. Materials and Methods: Sperm was collected from 4 Bangkok rooster chicken aged 1 year. Thirty native hens were used for insemination. Sperm from roosters was collected once a week and cryopreserved 24 h in liquid nitrogen containers at -196°C using 3% (P1), 5% (P2), 10% (P3), 14% (P4) and 18% (P5) DMA levels. Frozen thawed sperm quality was observed after thawing at 4° for 60 sec. The hens were slaughtered and sperm viability was recorded in their reproductive tract on 3, 7, 14 and 21 days after insemination. Results: The results showed that freezing decreased the sperm motility, viability and increased abnormalities. The P2 was observed to be the best DMA concentration for chicken sperm cryopreservation. Different DMA concentrations significantly (p<0.01) affected the motility, viability and abnormalities of frozen thawed sperm. Sperm motility (%) after thawing in P1, P2, P3, P4 and P5 were 38.00±9.08, 46.00±8.94, 16.00±5.48, 8.00±2.24 and 1.00±2.74, respectively. Sperm viability (%) after thawing was 40.10±7.21, 53.50±12.53, 22.00±4.43, 15.30±11.40 and 12.00±3.98 in P1, P2, P3, P4 and P5, respectively. Sperm abnormalities (%) after thawing in P1, P2, P3, P4 and P5 were 60.40±7.40, 41.00±3.32, 67.20±4.09, 68.20±8.58 and 71.00±11.64, respectively. In P2, spermatozoa from the hen’s reproductive tract (vagina, uterus, infundibulum and fimbria) were found motile up to 21 days after insemination. Conclusion: It can be concluded that the best DMA concentration for chicken sperm cryopreservation was 5% level in terms of sperm quality and its survivability in the reproductive tract of hen.
  Charles V. Lisnahan , Wihandoyo , Zuprizal and Sri Harimurti
  Objective: This study was conducted to determine the effect of cafeteria standard feed supplemented with methionine and lysine on the growth performance of native chickens during the grower phase (6-14 weeks). Methodology: A total of 240 six week-old native chickens were randomly divided into 4 treatments and 4 replications, with 15 birds in each replicate. The dietary treatments were: T0 (feed based on cafeteria standard), T1 (feed based on protein standard of NRC), T2 (cafeteria feed+0.10% methionine+0.25% lysine), T3 (cafeteria feed+0.23% methionine+0.55% lysine). Feed consumption, body weight gain and feed conversion ratio were measured over the 8 week growing period. At the end of the feeding trial, 32 birds were slaughtered and carcass percentages were determined. Results: Feed consumption of T0, T1, T2 and T3 were 2,671, 2,628, 2,722 and 2,805 g/bird/8 weeks, respectively. The body weight gain for the respective treatments was 692, 677, 747 and 780 g/bird/8 weeks whereas the feed conversion ratio was 3.86, 3.70, 3.65 and 3.60. The percentage of carcass for the four treatments was 59.50, 59.66, 61.36 and 61.55%, respectively. Conclusion: Treatment T3 that had supplementation with 0.23% methionine and 0.55% lysine produced the best growth performance.
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